RESUMO
Objective: To study the protective effect of resveratrol on lens epithelial cell apoptosis in diabetic cataract rat. Methods: A total of 84 Wistar rats were divided into 4 groups: 12 in Group A (control group), 24 in Group B (diabetic cataract group), 24 in Group C (therapeutic-dose of resveratrol group) and 24 in Group D (low-dose of resveratrol group). Rats in Group B-D were given with 60 mg/kg streptozotocin through intraperitoneal injection. Rats in Group C were given with 100 mg/kg resveratrol and rats in Group D were given with 20 mg/kg resveratrol. The caspase-3 expression levels and apoptosis ratios of LEC among each group were observed; the degrees of lens opacity in Group B-D after 12 weeks were compared. Results: There were significant differences in caspase-3 expression levels, apoptosis ratios of LEC among groups at 4 w, 8 w and 12 w (. P<0.05). After 12 weeks, in Group B the degree of lens opacity was as follow: 0 (0.00%) in grade I, 3 (37.50%) in grade II, 2 (25.00%)in grade III, 2 (25.00%)grade IV, and 1 (12.50%) in grade V; in Group C: 2 (25.00%)in grade I, 4 (50.00%) in grade II, 2 (25.00%)in grade III, 0 (0.00%)grade IV, and 0 (0.00 %) in grade V; in Group D: 1 (12.50%)in grade I, 4 (50.00%) in grade II, 2 (25.00%) in grade III, 1 (12.50%) grade IV, and 0 (0.00%) in grade V. The difference among Group B-D was statistically significant (. P<0.05). Conclusions: Resveratrol has protective effect on lens epithelial cell apoptosis in diabetic cataract rat, and the effect is relative to its dose.
RESUMO
OBJECTIVE@#To study the protective effect of resveratrol on lens epithelial cell apoptosis in diabetic cataract rat.@*METHODS@#A total of 84 Wistar rats were divided into 4 groups: 12 in Group A (control group), 24 in Group B (diabetic cataract group), 24 in Group C (therapeutic-dose of resveratrol group) and 24 in Group D (low-dose of resveratrol group). Rats in Group B-D were given with 60 mg/kg streptozotocin through intraperitoneal injection. Rats in Group C were given with 100 mg/kg resveratrol and rats in Group D were given with 20 mg/kg resveratrol. The caspase-3 expression levels and apoptosis ratios of LEC among each group were observed; the degrees of lens opacity in Group B-D after 12 weeks were compared.@*RESULTS@#There were significant differences in caspase-3 expression levels, apoptosis ratios of LEC among groups at 4 w, 8 w and 12 w (P<0.05). After 12 weeks, in Group B the degree of lens opacity was as follow: 0 (0.00%) in grade I, 3 (37.50%) in grade II, 2 (25.00%)in grade III, 2 (25.00%)grade IV, and 1 (12.50%) in grade V; in Group C: 2 (25.00%)in grade I, 4 (50.00%) in grade II, 2 (25.00%)in grade III, 0 (0.00%)grade IV, and 0 (0.00 %) in grade V; in Group D: 1 (12.50%)in grade I, 4 (50.00%) in grade II, 2 (25.00%) in grade III, 1 (12.50%) grade IV, and 0 (0.00%) in grade V. The difference among Group B-D was statistically significant (P<0.05).@*CONCLUSIONS@#Resveratrol has protective effect on lens epithelial cell apoptosis in diabetic cataract rat, and the effect is relative to its dose.
RESUMO
Objective:To study the protective effect of resveratrol on lens epithelial cell apoptosis in diabetic cataract rat.Methods:A total of84Wistar rats were divided into4 groups:12 inGroupA(control group),24 inGroupB(diabetic cataract group),24 inGroupC(therapeutic-dose of resveratrol group) and24 inGroupD(low-dose of resveratrol group).Rats inGroupB-D were given with 60 mg/kg streptozotocin through intraperitoneal injection.Rats inGroupC were given with100 mg/kg resveratrol and rats inGroupD were given with20 mg/kg resveratrol.The caspase-3 expression levels and apoptosis ratios ofLEC among each group were observed; the degrees of lens opacity inGroupB-D after12 weeks were compared.Results:There were significant differences in caspase-3 expression levels, apoptosis ratios ofLEC among groups at4 w,8 w and 12 w(P<0.05).After12 weeks, inGroupB the degree of lens opacity was as follow:0(0.00%) in grade Ⅰ,3(37.50%) in gradeⅡ,2(25.00%)in grade Ⅲ,2(25.00%)grade Ⅳ, and1(12.50%) in grade Ⅴ; inGroupC:2(25.00%)in grade Ⅰ,4(50.00%) in gradeⅡ,2(25.00%)in grade Ⅲ,0(0.00%)grade Ⅳ, and0(0.00%) in grade Ⅴ; inGroupD:1(12.50%)in grade Ⅰ,4(50.00%) in gradeⅡ,2(25.00%) in grade Ⅲ,1(12.50%) grade Ⅳ, and0(0.00%) in grade Ⅴ.The difference amongGroupB-D was statistically significant(P<0.05).Conclusions:Resveratrol has protective effect on lens epithelial cell apoptosis in diabetic cataract rat, and the effect is relative to its dose.