RESUMO
Objective:To investigate ameliorative effect of alcoholic extract of whole herb of Lucas cephalotes (L. cephalotes) on isoniazid and rifampicin induced hepatotoxicity. Methods: Hepatoprotective potential of alcoholic extract of L. cephalotes was ascertained at 200 mg/kg and 400 mg/kg in Sprague Dawley rats. Animals were intoxicated with isoniazid and rifampicin (100 mg/kg each, i.p.) for 21 d. Assessment was done on the basis of liver function test (serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase and bilirubin);effect on enzymes involved in oxidative stress (lipid peroxidase, superoxide dismutase, glutathione and catalase) and histology of liver were assessed as well. Comparison among different treatment group was done using One way ANOVA followed by Benferroni’s t test. Results: It was observed that at both of the selected doses extract exhibited significant ameliorative effect. In animals treated with the extract at both doses, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, bilirubin and lipid peroxidase level was found to be significantly less (P Conclusions:From the present study it can be concluded that alcoholic extract of L. cephalotes possesses significant hepatoprotective potential against isoniazid and rifampicin induced heptoxicity.
RESUMO
The whole plant of the methanolic extract from Leucas cephalotes was screened for invitro antioxidant (using the DPPH method), invivo analgesic (using hot plate test in mice) and anti-inflammatory (using rat paw edema test) activities. The methanolic extract of Leucas cephalotes (MELC) scavenged the DPPH radicals in a dose-dependent manner. The IC50 value to scavenge DPPH radicals was found to be 421.3µg/ml. A significant (p<0.0005) analgesic activity was observed at 60 min with 200 mg/kg, and 400 mg/kg exhibited maximum activity. The maximum anti-inflammatory response was produced at 3 hr and 2 hr with doses of 200 and 400 mg/kg, respectively. These results suggest that the methanolic extract from Leucas cephalotes exerts significant analgesic and anti-inflammatory effects, which were comparable with standard drugs.
O extrato metanólico total de Leucas cephalotes foi submetido à triagem para as atividades antioxidante in vitro (utilizando o método DPPH), analgésica (utilizando teste da placa quente, em camundongos) e antiinflamatória (utilizando teste de edema da pata de rato), nas doses de 200 e 400 mg/kg. O extrato metanólico de Leucas cephalotes (MELC) inativou radicais difenil picril hidrazila (DPPH) de forma dose-dependente. O IC50 para essa atividade foi de 421,3 µg/mL. Observou-se atividade analgésica significativa (p<0,0005) a 60 minutos, com 200 mg/kg, e, com 400 mg/kg, observou-se atividade máxima. A resposta antiinflamatória máxima foi produzida, respectivamente, em 3 h e 2 h, com doses de 200 e 400 mg/kg. Estes resultados sugerem que o extrato metanólico de Leucas cephalotes apresenta efeitos analgésico e antiinflamatório significativos, comparáveis aos fármacos padrão.