Chemical constituents from aerial parts of Ligusticum sinense cv. chaxiong / 中草药

Objective: To study the chemical constituents from the aerial parts of Ligusticum sinense cv. chaxiong. Methods: The chemical constituents were separated and purified by silica gel, ODS column chromatography, and preparative HPLC. Their structures were determined by various of spectroscopic analyses. Results: Fourteen compounds were isolated from the petrol ester and CH2Cl2 fractions of 95% ethanol extract of the aerial parts of L. sinense cv. chaxiong, and their structures were identified as 5-hydroxy-3- butylphthalide (1), 3-hydroxy-octa-1, 5-dien-7-one (2), umbelliferone (3), pumbinernoid A (4), crocinervolide (5), (3R, 6R, 7E)-3- hydroxy-4, 7-megastignadien-9-one (6), senkyunolide G (7), scopoletin (8), (2Z)-3-(3, 4-dihydroxyphenyl)-2-propenal (9), (3S, 5R, 6S, 7E, 9R)-5, 6-epoxy-3, 9-dihydroxy-7-megastigmene (10), vomifoliol (11), dihydroactinidiolide (12), 7-hydroxy-3-butylphthalide (13), and senkyunolide A (14). Conclusion: Compounds 1-13 are isolated from this plant for the first time, compounds 1, 2, 4-6, and 9-13 are found from the plants of genus Ligusticum L. for the first time, and compound 1 is a new natural product.

Comparison with differentiation of Ligusticum chuanxiong from its easily confusable Ligusticum sinense chaxiong / 中草药

Object　To provide a scientific basis for the identification and rational clinical use of the two herbal drugs. Methods　Experiments were carried out by comparing their properties, microscopic characteristics, UV spectra, TLC and GC chromatograms. Results　There are remarkable differences between the two crude drugs in these respects, and a convenient and effective method for their differentiation and identification has been established. Conclusion　Ligusticum sinense Hort. and Ligusticum sinense Oliv. cv. chaxiong Mass should not be used one instead of the other.

Comparison with differentiation of Ligusticum chuanxiong from its easily confusable Ligusticum sinense chaxiong / 中草药

Object To provide a scientific basis for the identification and rational clinical use of the two herbal drugs Methods Experiments were carried out by comparing their properties, microscopic characteristics, UV spectra, TLC and GC chromatograms Results There are remarkable differences between the two crude drugs in these respects, and a convenient and effective method for their differentiation and identification has been established Conclusion Ligusticum sinense Hort and Ligusticum sinense Oliv cv chaxiong Mass should not be used one instead of the other