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Objective: To prepare the liposomes carrying doxorubicin hydrochloride (DOX) and liquid fluorocarbon (PFH) in liquid form based liposomes (Lip-PFH-DOX), and to investigate the Lip-PFH-DOX nanoparticle enhanced ultrasound imaging and its therapeutic effect on human breast cancer MAD-MB-231 cells. Methods: Lip-PFH-DOX nanoparticles were prepared by using biocompatible phospholipid mixture as film-forming material and lipophilic DOX as additive. The morphology, particle size, potential and encapsulation efficiency of Lip-PFH-DOX nanoparticles were tested. Low intensity focused ultrasound was used to induce phase transition and rupture of nanoparticles. High performance liquid chromatography was used to detect drug release at different time points. The cytotoxic effect of the nanoparticles on human breast cancer MDA-MB-231 cells was detected and the apoptosis was observed. A nude mouse breast cancer mda-mb-231 model was established, and an appropriate amount of nanoparticles were injected into the tail vein to observe the enhanced ultrasound at the tumor site. Results: The liposomes loaded with DOX and PFH were successfully prepared. The particle size was (340.81±68.54)nm, the potential was (-17.72±7.66)mV, and the drug encapsulation rate was (86.80±2.55)%. Under transmission electron microscopy, the drug was successfully encapsulated in the liposomes. Under light microscopy, the liposomes were uniform in size, and the phase transition was occured. In vitro, the release rate of DOX of Lip-PFH-DOX reached 40.05±3.22 at 48 h. After 48 h, when the concentration was 100 μg/ml of Lip-PFH-DOX, the survival rate decreased to 45.00%. Lip-PFH-DOX and the nanoparticles can significantly enhance ultrasound development in vivo after low intensity ultrasound irradiation. Conclusion: The nanoparticles of DOX and PFH was successfully prepared, which can achieve ultrasound development in vivo and in vitro, and DOX can kill MAD-MB-231 cells.
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Objective To investigate the therapeutic effect of high intensity focused ultrasound (HIFU) ablation combined with homemade liquid fluorocarbon nanoparticles on cervical cancer in nude mice.Methods The cell experiment was divided into three groups:a,control group;b,HIFU group;c, HIFU + PFB nanoparticle group,and the viability of cells was detected using CCK-8 reagent.The mice were also divided into three groups:A,0.9% NaCI group;B,HIFU + 0.9% NaCI group;C,HIFU + PFB nanoparticle group. The tumors were removed and underwent triphenyl tetrazolium chloride(TTC) staining,and the necrosis area was measured.Histopathological changes of the tumors were examined by light microscopy.Results After HIFU irradiation,the viability rate of group c was (40.5 ±9.7)%,it was lower than that of group b (77.7 ±8.5)% (P <0.05) and that of group a(100 ±4.8)% (P <0.05). TTC staining of tumor showed a large scale of necrotic tissue in group C.The necrosis ratio of the three groups was 0%,(34.14±12.2)% and (65.97 ±25.1)%,respectively (P <0.05).HE staining showed karyorrhexis or an absence of nuclei in group B and group C,which demonstrated the coagulation necrosis. Conclusions HIFU ablation combined with liquid fluorocarbon nanoparticles can effectively treat the xenograft model of the human cervical carcinoma in nude mice.