HPLC fingerprint identification of Lonicera Japonica Flos and Lonicera Flos / 中草药

Objective: To compare the differences between Lonicera Japonica Flos and Lonicera Flos by establishing HPLC fingerprint and calculating the similarity. Methods: The columns was Phenomenex Luna 5 μm C18 (2) 100 A, 250 mm×4.6 mm; The column temperature was 40℃. The mobile phase was acetonitrile-0.5% phosphoric acid, the flow rate was 1 mL/min, and the wavelength was 350 nm. Results: HPLC fingerprint of Lonicera Japonica and similarity evaluation by screening large peak integration were established. The similarity of 12 batches of Lonicera Japonica Flos were all above 0.95, and four batches of Lonicera Flos were less than 0.80. Conclusion: HPLC fingerprint profiles under 350 nm can reflex the differences between Lonicera Japonica Flos and Lonicera Flos effectively; Similarity evaluation by screening large peak integration shows the tiny differences of chemical component.

Effects of GC7101, a Novel Prokinetic Agent on Gastric Motor Function: Ex Vivo Study

BACKGROUND/AIMS: GC7101, an extract of Lonicera Flos, is a novel developing drug for reflux esophagitis and functional dyspepsia. However, the drug's exact pharmacological mechanism of action remains unclear. This study assessed the effects of GC7101 on gastrointestinal (GI) motor function. METHODS: We used male guinea pigs to evaluate the effects of GC7101 on GI motility. The contraction of antral circular muscle in the presence of different doses of GC7101 was measured in a tissue bath. The prokinetic effects of GC7101 were tested using the charcoal transit assay from the pylorus to the most distal point of migration of charcoal mixture. To clarify the mechanism of action of GC7101, atropine, dopamine and the selective 5-hydroxytryptamine 4 receptor antagonist, GR113808 were used. RESULTS: The maximal amplitude of circular muscle contraction was induced by 5 mg mL(-1) GC7101. The area under the curve of contraction was significantly increased at 5 mg mL(-1) GC7101. Addition of 10(-6) M atropine, 10(-8) M dopamine or 10(-7) M GR 113808 to GC7101 5 mg mL(-1) decreased the amplitude and area under curve compared to GC7101 5 mg mL(-1) alone. GC7101 accelerated GI transit in a dose dependent manner except 100 mg kg(-1). Delayed GI transit caused by atropine, dopamine and GR 113808 was restored by GC7101 50 mg kg(-1). CONCLUSIONS: GC7101, an extract of Lonicera Flos, exerts a gastric prokinetic effect in guinea pig through cholinergic, antidopaminergic and serotonergic mechanisms. Therefore, GC7101 might be a novel drug for the treatment of functional dyspepsia.

Identification and attribution of Lonicerae Japonicae Flos and Lonicera Flos / 中草药

Objective: To identify Lonicerae Japonicae Flos and Lonicera Flos with various methods, using Lonicera japonica to represent Lonicera Flos for searching the different characteristics and discussing their attribution. Methods: An identification method on the basis of biological character comparison, structure observation of the flower epidermal by using SEM, and difference comparison of the marked components (chlorogenic acid, galu teolin, macranthoidin B, dipsacoside B) was established. The attribution of the two herbs on the account of Chinese Pharmacopoeia was discussed according to the historic using status and the present market situation. Results: In L. japonica, plenty of glandular hairs with turbinate top in flower epidermis and large leafy bracts in the bottom of flower were observed. Macranthoidin B and dipsacoside B were not detected. But in L. macranthoides, there were few leafy bracts and glandular hairs, and macranthoidin B and dipsacoside B were detected. Conclusion: Leafy bracts in the bottom of flower, glandular hairs in flower epidermis, and two characteristic components (macranthoidin B and dipsacoside B) could be used as basis for the identification of L. japonica and L. macranthoides.