RESUMO
OBJECTIVE:To establish a method for simultaneous determination of lysophosphatidyl choline(LPC)and lysophos-phatidyl ethanolamine(LPE)in Nimodipine fat emulsion. METHODS:HPLC was performed on the column of Lichrosher Diol with detector of evaporative light scattering detector with mobile phase A of heptane- isopropyl alcohol solution(43∶57,V/V),mobile phase B n-heptane-isopropyl alcohol-water(29.5∶59∶11.5,V/V/V)(gradient elution)at a flow rate of 1.5 ml/min,column tempera-ture was 40℃,and the injection volume was 20 μl. RESULTS:The linear range was 0.020 0-0.400 0 mg/ml for LPC(r=0.999 0)and 0.010 0-0.200 0 mg/ml for LPE(r=0.999 6),and the logarithm value of concentration and peak area showed good linear relationship;the limit of quantitation was 0.013 4 mg/ml for LPC and 0.013 0 mg/ml for LPE;the limit of detection was 0.007 8 mg/ml for LPC and 0.007 6 mg/ml for LPE;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 95.96%-100.63%(RSD=1.83%,n=9)and 99.22%-101.76%(RSD=0.80%,n=9). CONCLUSIONS:The method is simple,and can be used for the determination of related substance in Nimodipine fat emulsion.
RESUMO
Objective: To explore the brain damage effects of lysophasphatidyl choline(LPC) in rats with pancreatitis and explore the pathogenesis of pancreatic encephalopathy(PE).Methods: SD rats were divided into test group,control group one and control group two at random.Acute edematous pancreatits rat model was induced following Aho HJ method for test group and control group one,then test group rats were venously applied with LPC and control group one was injected saline through tail vein.Control group two was treated with venous injection LPC through the rat tails without operation.Horseradish peroxidase(HRP) was used as a tracer to detemine if the blood-brain barrier(BBB)was open 7～10 days after successive application of LPC.The extravasated tracer was showed by diaminobenzidine(DAB).Rat brain tissue sections were examined by ponceau stain and Luxol Fast Blue stain to determine whether the rat brains were demyelination at the same time.Results: The BBB permeability of the test groupLTU rats increased greatly and obvious demyelination was observed in test group rat brains while both control groups had basically intact BBB and scarce demyelination was observed in both control groups.A statistical difference existed between the test group and control groups.Conclusion:LPC definitely open the BBB of the rat pancreatitis and demyelinates the brain of pancreatitis rats thus LPC plays an important role in the pathogenesis of PE.