The effect of methylprednisolone on the expression of glial fibrillary acidic protein and proliferating cell nuclear antigen in M?ller cells of rats' retinae injured by laser / 中华眼底病杂志

Objective To investigate the effect of methylprednisolone on the expression of glial fibrillary acidic protein (GFAP) and proliferating cell nuclear antigen (PCNA) in M?ller cells of rats' retinae injured by laser. Methods Forty SD rats were randomly divided into two groups and inflicted with laser photocoagulation.The rats in treatment group were given methylprednisolone by intraperitoneal injection with a dose of 30 mg/kg for 3 days.At the 3rd,7th,14th,and 28th day after photocoagulation respectively, the eyes were enucleated,fixed and cut into sections.Immunohistochemical examination was used to detect the expression of PCNA and GFAP. Results After photocoagulation the M?ller cells expressed PCNA both in the treatment and control group,and the expression of PCNA decreased sharply after 3 days. The expression of PCNA in treatment group was less than that in control group. After photocoagulation the M?ller cells also expressed GFAP and the expression of GFAP lasted for at least 28 days ,and the expression of GFAP expression in the treatment group was less than that in the control group. Conclusion Methylprednisolone can reduce the expression of GFAP and PCNA in M?ller cells of rats' retinae injured by laser.

Effects of taurine on high-altitude hypoxia induced changes of retina morphology and SDH,LDH activity / 第三军医大学学报

Objective To investigate retina structure and function under the condition of acute continuous hypoxia at high altitude and study on the protective effects of taurine on retina injury. Methods Low-pressure chamber was used to simulate 4 500 m and 5 500 m altitude.A total of 126 SD rats were used in this study,half assigned to the conditions under plain,4 500 m and 5 500 m,the other half before the same assignment fed with 2.4% taurine in plain for a week.At 2,6,12,24,48,72 h after entering the chamber,the eyes of SD rats were taken out,fixed and sectioned.Retina layer structure and ultrastructure of retina M?ller cells were observed by light microscope and transmission electron microscope.The activity of succinate dehydrogenase(SDH) and lactate dehydrogenase(LDH) were detected with enzymohistochemistry.Results INL,IPL and GCL were injured in low oxygen.In the early period,migrating IPL cells increased(P