Influence of Biological Pesticide on Fruting Rate and Yield of Amomum villosum Lour / 广州中医药大学学报

[Objective] To observe the influence of different kinds of biological pesticide on the fruiting rate and yield of Amomum villosum Lour. (AVL) . [Methods] Medicinal plant field experiment method was used to observe the influence of biological pesticide A (highly effective immuno-biologic bactericide, 75.76mg?m-2 for one time) and its 1000-fold, 800-fold and 500-fold diluent on the fruiting rate and yield of AVL. Meanwhile, the influence of biological pesticide B (edible oligosaccharide, 30.30mg?m-2 for one time) and biological pesticide C (deguelin emulsion, 1.89mg?2m-2 for one time) was also observed. [Results] The three biological pesticides, as well as the diluents of biological pesticide A increased the fruiting rate and yield of AVL, the influence of the moderate-concentration biological pesticide A being the greatest. [Conclusion] Proper application of biological pesticide is one of the important ways to raise the yield of AVL.

In-Vitro Culture of Different Explants from Andrographis paniculate (Burm. f.) Nees / 广州中医药大学学报

[ Objective ] To supply a technological evidence for the rapid reproduction of Andrographis paniculata (Burm. f.) Nees (APN). [Methods] Different explants from sterile seedling of Andrographis paniculata (Burm. f.) Nees were cultured in vitro to induce the production of fascicular buds. [ Results ] Cotyledon with nodes and half cotyledon with nodes were the most suitable explants for culture, and the budding rate was 100% and over 90% respectively. 6-Benzylaminopurine (BA) in the concentrations of 0.5 ~ 1.0mg/L were beneficial for the reproduction and growth of buds. The age of explants had no correlation with budding. The optimal rooting medium was MT (Murashige and Tucher) medium added with Img/L NAA (naphthaleneacetic acid) or IBA (indolebutyric acid). A highest rooting rate as much as 61.9% was achieved after 10 days of culture. [Conclusion] An effective plant regeneration system has been established for explants of Andrographis paniculata (Burm. f.) Nees.

Review and Prospects: Development and Utilization of Herbal Medicinal Resourses in South China / 广州中医药大学学报

The development and utilization of herbal medicinal resourses in south China were reviewed from the following aspects: (1) resources investigation; (2) introduction of South China herbal medicine (SCHM) to North China; (3) culture technology of green herbal medicine; (4) building of the base of good agriculture practice; (5) species study and quality evaluation of SCHM, and (6) product development of SCHM. It is concluded that the research on development and utilization of SCHM contributes a lot to reducing SCHM import, saving foreign exchange and ensuring the safety, effect, output stability and quality control in the production of medicinal material.

Histocytological Observation of Somatic Embryogenesis in In-Vitro Cultured Dendrobium candidum wall. Ex Lindl / 广州中医药大学学报

Objective To observe the somatic embryogenesis in in-vitro cultured Dendrobium candidum wall.Ex Lindl.(DCWL),and to supply evidence for its rapid propagation and germplasm preservation.Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants,N6 was used as the basic culture with phytohormone added,and fungal extracts as the elicitor.Protocorm-like bodies and callus of DCWL were used for induction culture.Results Somatic embryogenesis in in-vitro cultured DCWL derived from the epithelial cells or inner cells of callus of DCWL.Conclusion A large amount of buds can be obtained by the induction and culture of protocorm-like bodies and callus of DCWL.

Effect of Different Storage Methods on Germination Capacity of Aquilaria sinensis(Lour.) Gilg / 广州中医药大学学报

【Objective】To devise the optimal method and the optimal water content for the storage of seeds of Aquilaria sinensis(Lour.) Gilg..【Methods】The seeds of Aquilaria sinensis(Lour.) Gilg.were stored in plastic bag,drying apparatus,wet sand and dry sand separately.And then the germination capacity of the seeds with different water content was detected at 4℃ and room temperature(30℃) in different time.【Results】The germination capacity of Aquilaria sinensis(Lour.) Gilg.decreased with the prolongation of storage time and with the decrease of water content.The seeds with water content being 9.34% and 7.35% had a higher germination capacity when stored at 4℃ for 35 days than that stored at room temperature.The fresh seeds stored in wet sand sprouted and the seeds stored in drying apparatus and dry sand had lower germination capacity and went mouldy.【Conclusion】Low temperature and moderate water content are benificial to the storage of the seeds of Aquilaria sinensis(Lour.) Gilg..

Tissue Culture and Plant Regeneration of Aristolochia contorta Bge / 广州中医药大学学报

[Objective] To explore the culture conditions of inducing callus from tissue of Aristolochia contorta Bge (ACB). [Methods] The leaves of ACB were used as the explants. Basic medium (including 1/2 MS medium, MS medium and modified MS medium) containing corresponding phyto-hormones was applied for the induction of ACB callus. The influerices of different culture conditions such as three kinds of basic medium, different pH values and addition of different kinds of phyto-hormones at different concentrations into the basic medium respectively or simultaneously, on the callus induction of ACB were observed. [Results] (1) After the three kinds of basic medium were added with the phytohormones of 0.1mg/L kinetin (KT) and 0.2mg/L ?-naphthalene acetic acid (NAA) , a large amount of ACB calluses were induced in the MS medium and modified MS medium, while ACB calluses did not occur in the 1/2 MS medium. (2) When the pH value of the culture medium composed of modified MS medium and 0.1mg/L KT and 0.2mg/L NAA was at 5.8, 7.0 and 8.0, a large amount of ACB calluses were induced in the medium with pH value being 7.0 and 8.0 while a few calluses occurred in the medium with pH value being 5.8. (3) ACB calluses were induced in modified MS medium with NAA added , but calluses did not occur in modified MS medium with KT added. When the modified MS medium was added with different kinds of phyto-hormones at different concentrations, ACB calluses were loose in the medium with high-concentration NAA and this did not benefit to the differentiation of buds for too fast cell division, and the callus induction rate was low in the medium with low-concentration NAA. The optimized culture medium was modified MS medium with 1mg/L KT and 0.5mg/L NAA added. [Conclusion] The optimum culture conditions of inducing callus from ACB tissues are: MS medium or modified MS medium with 1mg/L KT and 0.5 mg/L NAA added being the culture medium, and pH value being 7.0-8.0.