Cadaveric study of portal and hepatic venous anatomy with special reference to territory of middle hepatic vein

Background: Liver transplantation has become the standard therapy for end-stage chronic liver disease and acute hepatic failure. The shortage of cadaveric donor organs deceased donor liver transplant (DDLT) has led to the development of living donor liver transplantation (LDLT). In LDLT the concept is based on the potential regenerative power of the human liver.Methods: This was an observational study done in department of surgery, Gandhi Medical College and Hamidia Hospital, Bhopal on 50 cadaveric liver specimens and dissection was carried out in department of Anatomy from March 2018 to February 2019.Results: In this study 50% of the specimens had all the three hepatic veins, while the remaining 50% had two hepatic veins: the right and left. The presence of one or more right accessory hepatic veins draining the right lobe was observed in all the cases. In most of the livers the LHV and MHV formed a common trunk, which joined the IVC (76.0%). In some cases, they drained independently into IVC (18%). In the present study of 38 adult cadaveric livers, termination of PV was observed as extra hepatic in 89.47 livers, Intrahepatic in 2.63% and at the capsule in 7.89% livers.Conclusions: There are three main hepatic veins: RHV, MHV and LHV. In this study 50% of the specimens had all the three hepatic veins, while the remaining 50% had two hepatic veins: the right and left. Thus significant variation was seen and it could definitely be helpful to hepatobilliary surgery and in liver transplant.

A prospective observational study of branching pattern of middle hepatic vein on contrast enhanced computed tomography

Background: The main purpose of pre-operative imaging in liver transplantation is to expose the arterial and venous vascular map. Prior to transplantation it is necessary to image the vascular structures property due to complex nature of liver vascular anatomy and its frequent variations. CT is a useful method not only in the determination of hepatic arterial anatomy, hepatic venous anatomy, accessory hepatic veins and portal veins variations.Methods: This was a prospective observational study performed at the department of surgery, Gandhi Medical College Bhopal and study of CECT was carried out in the department of radiodiagnosis with approval from college ethical committee on 100 patients during a period of 2 years from 2017 to 2019.Results: In study of 100 cases were studied. The study mainly done on the MHV mainly its length, width draining segments of liver by MHV and accessory hepatic veins. In overall cases maximum cases had drainage from segment IVb, V and segment VIII. The segment 5 accessory vein is the most common single accessory vein found in the study.Conclusions: Many parameters of MHV have studied in the study and understanding of these parameters is undoubtedly important for operating surgeon its anatomical draining patterns, draining liver segments and has got the presence of the various accessory veins in the liver. Recognition of these accessory hepatic veins is important because some of these accessory veins may become useful in segmental liver transplantation.

Effects of transport and storage conditions on the nucleic acid detection for three kinds of mouse RNA viral samples / 中国比较医学杂志

Objective To analyze the effect of transport and storage conditions on the detection of pathogenic nucleic acid MHV, Reo-3, MNV in laboratory mouse cecal contents samples. Methods MHV, Reo-3 and MNV were mixed with mouse cecal contents and used as reference samples,respectively. They were placed in the lysis buffer of RNA extraction reagent(buffer AVL)or normal saline, and stored at 4℃ and room temperature(22℃-25℃). RNA of these samples was extracted at 1,2,3,7,and 14 days. Then the amount of nucleic acid in samples was detected by real-time fluorescence quantitative PCR. Results A greater decrease of the amount of nucleic acid was observed when the samples were placed in normal saline than that kept in buffer AVL. The amount of nucleic acid in samples stored at 4℃ was found to be higher than that stored at 25℃ room temperature. The amount of nucleic acid in the samples which were kept in buffer AVL at 4℃ for 3 days was higher than 50%,still detectable in the samples kept for 7 days,and undetectable at 14 days. Conclusions Mouse cecal content samples are preferably stored in the lysis buffer of RNA extraction reagent and transported at 4℃ for the detection of MHV, Reo-3, and MNV nucleic acid. It is better to complete the detection test within 3 days.

Construction and Genetic Analysis of Murine Hepatitis Virus Strain A59 Nsp16 Temperature Sensitive Mutant and the Revertant Virus / 中国病毒学·英文版

Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study, we created a MHV-A59 temperature sensitive (ts) mutant Wu"-ts18(cd) using the recombinant vaccinia reverse genetics system. Virus replication assay in 17C1-1 cells showed the plaque phenotype and replication characterization of constructed Wu"-ts18(cd) were indistinguishable from the reported ts mutant Wu"-ts 18. Then we cultured the ts mutant Wu"-ts 18(cd) at non-permissive temperature 39.5℃, which "forced" the ts recombinant virus to use second-site mutation to revert from a ts to a non-ts phenotype. Sequence analysis showed most of the revertants had the same single amino acid mutation at Nsp16 position 43. The single amino acid mutation at Nsp16 position 76 or position 130 could also revert the ts mutant Wu"-ts 18 (cd) to non-ts phenotype, an additional independent mutation in Nsp13 position 115 played an important role on plaque size. The results provided us with genetic information on the functional determinants of Nsp16. This allowed us to build up a more reasonable model of CoVs replication-transcription complex.

Expression of Prothrombinase/fibroleukin Gene fg12 in Lung Impairment in a Murine Severe Acute Respiratory Syndrome Model / 中国病毒学

To evaluate the role of murine fibrinogen like protein 2 (mfgl2) /fibroleukin in lung impairment in Severe acute respiratory syndrome (SARS), a murine SARS model induced by Murine hepatitis virus strain 3 (MHV-3) through trachea was established. Impressively, all the animals developed interstitial pneumonia with extensive hyaline membranes formation within alveoli, and presence of micro-vascular thrombosis in the pulmonary vessels. MHV-3 nucleocapsid gene transcripts were identified in multiple organs including lungs, spleen etc. As a representative proinflammatory gene, mfgl2 prothrombinase expression was evident in terminal and respiratory bronchioles, alveolar epithelia and infiltrated cells in the lungs associated with fibrin deposition and micro-vascular thrombosis. In summary, the established murine SARS model could mimic the pathologic characteristics of lungs in patients with SARS. Besides the physical damages due to virus replication in organs, the up-regulation of novel gene mfgl2 in lungs may play a vital role in the development of SARS associated lung damage.

A Primary Study of the Subgroups of T Lymphocytes in MHV-3 Induced Chronic Viral Hepatitis / 中国病毒学

To study the contribution of T cell subsets in the pathogenesis of Murine hepatitis virus Type3 (MHV-3) induced chronic viral hepatitis in C3H/Hej mice, ninety C3H/Hej mice were chosen to individually receive 10 plaque forming units (PFU) of MHV-3 intraperitoneally. The changes of virus titer and pathology in liver tissue were examined by standard plaque assay and by the hematoxylin/eosin (HE) staining method from 2 days post MHV-3 infection. The ratios of T cell subsets including CD3+CD4+CD8-, CD3+CD4-CD8+, CD3+CD4-CD8-, CD3+CD4+CD25+, CD3+CD4+CD25- and CD3+CD4-CD25+ T lymphocyte of total T lymphocytes in blood, spleen and liver were examined at 0, 2, 4, 6,8, 10, 12, 15, 20, 25, 30, 40 days post MHV-3 infection by flow cytosorting. We observed that the virus titer raised and showed persistent virus duplications and inflammatory changes in the livers of C3H/Hej mice from 2 days post MHV-3 infection. The double negative T cell (DN Treg cell) and CD4+CD25+ T cell ratios increased significantly from 2 days post MHV-3 infection in C3H/Hej mice, and CD3+CD4+CD8-, CD3+CD4-CD8+, CD3+CD4+CD25- and CD3+CD4-CD25+ T cell ratios decreased accordingly. In conclusion, the changes of virus titer and pathology in the livers of C3H/Hej mice post MHV-3 suggest their contribution to viral persistence. Further characterizations of DN Treg cells are that infection indicates that MHV-3 could induce the chronic inflammation in livers of C3H/Hej mice.The increase of the DN Treg cell and CD4+CD25+ T cell ratios in C3H/Hej mice post MHV-3 infection suggests that DN Treg cells and CD4+CD25+ T cells may both have important suppressive immunomodulation functions in the development of chronic viral hepatitis and have important roles in the virus persistent infection. Further characterizations of DNT cell and CD4+CD25+ T cell are under investigation.