Caracterización epidemiológica de las exposiciones a dióxido de cloro/clorito de sodio en el contexto de la pandemia COVID-19: Reporte de los centros toxicológicos de América Latina / Epidemiological characterization of exposures to chlorine dioxide/ sodium chlorite in the context of the COVID-19 pandemic: Report of Poison Control Centers in Latin America

Resumen Los centros de información y asesoramiento toxicológico CIATs de América Latina, en el contexto de la pandemia de COVID-19, recibieron una serie de llamadas para consultas y asesoramientos relacionados con el uso de dióxido de cloro/clorito de sodio, que se estaban empleando en el tratamiento o prevención de dicha enfermedad. Dentro de la legislación vigente en los países de América Latina, no se contemplan productos farmacéuticos registrados para uso en humanos, ni se tiene evidencia de registros sanitarios en Europa, Canadá o Estados Unidos para tal fin, que contengan dióxido de cloro o clorito de sodio. Esta publicación, compila la información registrada como parte de la estadística del trabajo de ocho CIATs correspondientes a igual número de países de América Latina. Se identificó sexo, edad, sintomatología, circunstancia y grado de severidad de los 56 casos de pacientes intoxicados con dióxido de cloro/clorito de sodio registrados en el período del 15 de marzo al 30 de septiembre de 2020 en estos ocho países. Los resultados obtenidos confirman que la causa más común fue por mal uso, y el lugar de ocurrencia fue el hogar o sus alrededores, siendo el mayor porcentaje adultos jóvenes comprendidos entre 30 y 49 años. Los síntomas de intoxicación más frecuentemente encontrados fueron gastrointestinales, seguidos de cardiovasculares y respiratorios. La vía de ingreso al organismo en la mayoría de los casos fue por vía oral, reportándose algunos casos por vía inhalatoria, y en el 50% de los casos se constituyeron casos de severidad moderada, severa o fatal (3 fallecimientos). Este estudio contribuye a generar información relevante para las diferentes autoridades sanitarias, los ministerios de salud, las entidades encargadas de inspección, vigilancia y control de los países en los que se comercializan estos productos de manera ilegal por medio de redes sociales y promoviéndolos para uso en humanos para prevenir o curar COVID-19.

Abstract The Poison Control Centers in Latin America, in the context of COVID-19 pandemic, received a series of calls for consultations and recommendations related to the use of chlorine dioxide/sodium chlorite, in the treatment or prevention of CO-VID-19. Under current legislation in Latin America, no pharmaceutical products are registered for use in humans that contain chlorine dioxide or sodium chlorite, nor is there evidence of sanitary registries in Europe, Canada, or the United States for this purpose. This publication compiles the information registered by eight Poison Control Centers that correspond to the same number of Latin American countries. Sex, age, symptoms, circumstance, and degree of severity of the 56 cases of patients poisoned with chlorine dioxide/ sodium chlorite registered in the period from March 15th to September 30th, 2020 were identified. The results obtained confirm that the most common cause of poisoning was unintentional misuse, all of which occurred at home or its surroundings, with the highest percentage of registered cases being young adults between 30 and 49 years old. The most frequent symptoms of intoxication were gastrointestinal, followed by cardiovascular and respiratory. The route of exposure in most cases was oral, with some cases reported by inhalation; 48.2% of the cases were of moderate, severe, or fatal (3 deaths). This study contributes to the generation of relevant information for different health authorities, ministries of health, entities in charge of inspection, surveillance, and control in countries where these products are illegally marketed through social networks and promoted for use in humans to prevent or cure COVID-19.

Succinylcholine Induced Masseter Muscle Spasm during Rapid Sequence Induction in a Pregnant Patient with Kypho- Scoliosis: Case Report

Isolated masseter spasm is a rare entity encountered in patients with neuromuscular disorders and is frequently associated with use of succinylcholine or halogenated inhalational anaesthetic during induction. We present a case of 32-year old primigravida obstetric patient with kypho-scoliosis posted for lower segment caesarean section that developed masseter muscle spasm (MMS) following administration of a standard dose of succinylcholine. The episode resolved spontaneously after 6-7 min without progression to malignant hyperthermia. The report highlights that an event of masseter muscle spasm, though, rarely encountered in life-time practice of an anaesthesiologist, may be successfully managed by maintaining oxygenation and ventilation till the crisis is tided over

Interaction between MMS2 and P53 on proliferation and apoptosis of colon cancer cells / 中国免疫学杂志

Objective:To explore the regulatory effects of proliferation and apoptosis on THC-8307 by MMS2 siRNA and P53 siRNA.Methods:We experimentally suppressed the MMS2 and P53 expression in human colon cancer cells by the interference RNA technology ( RNAi) as monitored by Real-time qRT-PCR and Western blot.THC-8307 cells that express rate significantly reduced were collected as case group , while using untreated cells as the blank control group , and mock-treated cells as the negative control group.After separately interfering the target genes in each group ,test the relationship and expression level of the two genes.Utilizing flow cytometry techniques to test cells proliferation and apoptosis rate of each group.Results: Compared to the control group , colon cancer cells in which MMS2 and P53 were silenced displayed significant increase of P53,MMS2 mRNA and protein levels(P<0.05).MMS2-depleted cells displayed increase in apoptosis rates ,for both early and later stages ( P<0.05 ).Conclusion: MMS2 and P53 negatively regulate each other in colon cancer cells proliferation and apoptosis .

Practice of Optimizing Outpatient Service Process Using the Integrated Outpatient Self-service Platform / 医学信息学杂志

The paper conducts a case study of Yangzhou No.1 People′s Hospital, introduces the practice of outpatient process opti-mization based on informatization support platform, analyzes the operation status of self-service outpatient service platform and existing problems.Based on the M/M/s model in queuing theory, the queuing model of payment for outpatient service in the hospital is construc-ted, the characteristics of queuing payment problems for outpatient service are revealed, the relationship between the sharing rate of self-service terminals and the average length of the queue in service windows, the average waiting time of patients.

Construction of fusion gene VH-mms13 and identification of its protein / 中国生化药物杂志

Objective To construct the prokaryotic expression vector pET30a( +)-VH-mms13 and identification of its protein after induced with IPTG.Method Heavy chain variable region VH gene of typeⅣcollagenase monoclonal antibody and magnetosome membrane protein gene mms13 were amplified separately,the fusion gene VH-linker-mms13 were synthesized by SOE-PCR technique and inserted into pET30a ( +) plasmid, which was confirmed by restriction enzyme digest and sequencing.Then the recombinant plasmid pET30a ( +)-VH-mms13 was transform into E.coli DE3 and induced with 0.4 mmol/L IPTG.The fused protein was identified by SDS-PAGE and Western blot.Results The length of fusion gene VH-mms13 was 738 bp,and the sequence was correct.After induced with IPTG,the fused protein was found in the inclusion body and Western blot results suggested that the fused protein can bind with His-tag antibody specifically.Conclusion Expression vector pET30a ( +)-VH-mms13 is successfully constructed and the fusion protein has good immunogenicity,which lay the foundation for the development of biomagnetism-targeted drug.

Effectiveness of Preferable Color Light on Psychosomatic State Evaluated by Emotional Parameters and Biochemical Parameters / 日本補完代替医療学会誌

<b>Objective</b>: To assess the effectiveness of preferred colored light on psychosomatic state.<br> <b>Methods</b>: Preferred color light and other colored light were projected on a screen and shown to experimental and control subjects. To determine the effect of such light, mood adjective scores as determined by the Multiple Mood Scale (MMS) were measured as an emotional parameter, and levels of salivary chromogranin A (CgA) and salivary immune globulin A were measured as biochemical parameters. This study was performed in a randomized, crossover design. These data were analyzed statistically and a<i> p </i>value less than 0.05 was considered significant.<br> <b>Results</b>: After being exposed to preferred colored lights, mean MMS scores indicating positive moods, such as well-being increased significantly (p = 0.025), and scores indicating negative moods, such as depression and boredom decreased significantly (p = 0.005, p = 0.041). Mean value of salivary CgA also decreased and was significantly different between experimental and control group (p < 0.001).<br> <b>Conclusion</b>: Preferred colored light may be effective in promoting a calm positive state.<br>

Effects of Color Images on Psychosomatic State-Effectiveness of Color Images as Mood Stimulants as Assessed by Using an Image Selection System- / 日本補完代替医療学会誌

<i>Objective</i>: To assess the effectiveness of color images as mood stimulants by using an image selection system.<br> <i>Methods</i>: Color images of flowers and seascapes, each mainly comprising of 5 colors, were arranged into a set of 50 images (10 images for each of 5 colors) on a personal computer and shown to 40 university students on stressful days during (i) an examination period and (ii) a non-examination period. The students selected color images according to their individual preferences by using an image selection system. To determine the effect of viewing the color images, mood adjective scores as determined by the Multiple Mood Scale (MMS) were measured as an emotional parameter, and the levels of salivary chromogranin A (CgA) and salivary cortisol were measured as biochemical parameters. These data were analyzed statistically and a <i>p</i> value less than 0.05 was considered significant.<br> <i>Results</i>: After viewing the color images, mean MMS scores indicating positive moods, such as well-being and friendliness increased significantly (p<0.05), and scores indicating negative moods, such as depression, boredom and hostility decreased significantly(p<0.05). The mean value of salivary CgA also decreased significantly after viewing the images (p<0.05).<br> <i>Conclusion</i>: Color images could possibly be used to induce a parasympathetically dominant state.<br>

Antimutagenic activity of cashew apple (Anacardium occidentale Sapindales, Anacardiaceae) fresh juice and processed juice (cajuína) against methyl methanesulfonate, 4-nitroquinoline N-oxide and benzo[a]pyrene

Cashew apple juice (CAJ), produced from the native Brazilian cashew tree (Anacardium occidentale), and has been reported to have antibacterial, antifungal, antitumor, antioxidant and antimutagenic properties. Both the fresh unprocessed juice and the processed juice (cajuína in Portuguese) has been shown to consist of a complex mixture containing high concentrations of anacardic and ascorbic acids plus several carotenoids, phenolic compounds and metals. We assessed both types of juice for their antimutagenic properties against the direct mutagens methyl methanesulfonate (MMS) and 4-nitroquinoline-N-oxide (4-NQO) and the indirect mutagen benzo[a]pyrene (BaP) using pre-treatment, co-treatment and post-treatment assays with Salmonella typhimurium strains TA100, TA102, and TA97a. In pre-treatment experiments with strains TA100 and TA102 the fresh juice showed high antimutagenic activity against MMS but, conversely, co-treatment with both juices enhanced MMS mutagenicity and there was an indication of toxicity in the post-treatment regime. In pre-, co-, and post-treatments with TA97a as test strain, antimutagenic effects were also observed against 4-NQO and BaP. These results suggest that both fresh and processed CAJ can protect the cells against mutagenesis induced by direct and indirect mutagens.

Detection for DNA Damage in Human Lymphocytes Induced by Four Chemicals Using Comet Assay / 环境与健康杂志

Objective To validate feasibility of comet assay as a tool for detecting DNA damage induced by various types of chemical mutagens.Study of DNA damage induced by4chemicals on human lymphocytes was carried out in vitro.Methods Human lymphocytes were exposed to4-nitroquinoline-1-oxide(4NQO,a UV-mimetic agent ),methyl methanesulfonate(MMS,an alkylating agent ),Bleomycin(BLM,a radiamimetic agent )and Mitomycin(MMC,a DNA crosslink agent )for3h,the DNA single strand breaks(SSB)induced by4chemicals were measured immediately(0h-incubation)and21h-incubation after3h-exposure to the chemicals with comet assay.Results It was found that the SSB induced by4NQO,MMS and BLM,which revealed a dose-response relationship(P