Preparation of two tanshinone Ⅱ_A-astragaloside Ⅳ co-loaded nano-delivery systems and in vitro antitumor activity comparison / 中国中药杂志

This study screened excellent carriers for co-loading tanshinone Ⅱ_A(TSA) and astragaloside Ⅳ(As) to construct antitumor nano-drug delivery systems for TSA and As. TSA-As microemulsions(TSA-As-MEs) were prepared by water titration. TSA-As metal-organic framework(MOF) nano-delivery system was prepared by loading TSA and As in MOF by the hydrothermal method. Dynamic light scattering(DLS), transmission electron microscopy(TEM), and scanning electron microscopy(SEM) were used to characterize the physicochemical properties of the two preparations. Drug loading was determined by HPLC and the effects of the two preparations on the proliferation of vascular endothelial cells, T lymphocytes, and hepatocellular carcinoma cells were detected by the CCK-8 method. The results showed that the particle size, Zeta potential, and drug loading of TSA-As-MEs were(47.69±0.71) nm,(-14.70±0.49) mV, and(0.22±0.01)%, while those of TSA-As-MOF were(258.3±25.2) nm,(-42.30 ± 1.27) mV, and 15.35%±0.01%. TSA-As-MOF was superior to TSA-As-MEs in drug loading, which could inhibit the proliferation of bEnd.3 cells at a lower concentration and improve the proliferation ability of CTLL-2 cells significantly. Therefore, MOF was preferred as an excellent carrier for TSA and As co-loading.

Research progress of AMPK regulating ferroptosis related signaling pathway / 中国药理学通报

Energy imbalance is eonsidered an important driver of human diseases. As an important energy sensor, AMPK plays a central role in maintaining energy homeostasis, making it a key target for disease prévention and treatment. As a new type of cell death, ferroptosis is related to the pathophysiologieal process of many diseases, and AMPK-related signaling pathway is an important way to regulate iron death. Understanding which pathway AMPK induces or inhibits ferroptosis will provide new ideas for the treatment of diseases in the future and provide new targets for new drug research. In this paper, we review the related litera-tures and explore the signaling pathways of AMPK regulating ferroptosis, wïth a brief description of the application of AMPK-mediated ferroptosis related signaling pathways in diseases, hoping to provide reference for later research.

MIL-53-based homochiral metal-organic framework as a stationary phase for open-tubular capillary electrochromatography / 药物分析学报

Homochiral metal-organic frameworks(MOFs)have attracted considerable attention in many fields of research,such as chiral catalysis and chiral chromatography.However,only few homochiral MOFs can be effectively used in capillary electrochromatography(CEC)and their performances are far from adequate.In this study,we successfully synthesized achiral nanocrystalline MIL-53.A facile post-synthetic modi-fication strategy was then implemented to functionalize the product,yielding a homochiral MOF:L-His-NH-MIL-53.This MOF was then employed as a chiral coating in open-tubular CEC mode(OT-CEC),and,as such,it exhibited high enantioselectivities for several racemic drugs.The homochiral MOF and the fabricated capillary coating were systematically characterized using transmission electron microscopy,scanning electron microscopy(with energy-dispersive X-ray spectrometry),Fourier-transform infrared spectroscopy,X-ray diffractometry,thermogravimetric analysis,circular dichroism spectroscopy,Bru-nauer-Emmett-Teller surface area measurements,and X-ray photoelectron spectroscopy.This study is expected to provide a new strategy for the design and establishment of MOF-based chiral OT-CEC systems.

A homogenous nanoporous pulmonary drug delivery system based on metal-organic frameworks with fine aerosolization performance and good compatibility

Pulmonary drug delivery has attracted increasing attention in biomedicine, and porous particles can effectively enhance the aerosolization performance and bioavailability of drugs. However, the existing methods for preparing porous particles using porogens have several drawbacks, such as the inhomogeneous and uncontrollable pores, drug leakage, and high risk of fragmentation. In this study, a series of cyclodextrin-based metal-organic framework (CD-MOF) particles containing homogenous nanopores were delicately engineered without porogens. Compared with commercial inhalation carrier, CD-MOF showed excellent aerosolization performance because of the homogenous nanoporous structure. The great biocompatibility of CD-MOF in pulmonary delivery was also confirmed by a series of experiments, including cytotoxicity assay, hemolysis ratio test, lung function evaluation,

Clinical observation on supplemented shenfu decoction combined with western medicine in the treatment of multiple organ failure in the elderly / 中国生化药物杂志

Objective To explore the effect of Shenfu Decoction combined with western medicine treatment of elderly patients with multiple organ dysfunction syndrome (MOF) clinical effect. Methods 65 cases of elderly patients with MOF as the research object, were randomly divided into observation group (n=35) and control group (n=30). The two groups were given conventional standard western medicine treatment, the observation group on the based on the combination of Shenfu Decoction treatment. Results After treatment, two groups of blood pressure and urine volume were significantly increased (P<0.05), and the observation group was significantly higher than the control group (P<0.05); the two groups of patients with respiratory and heart rate were significantly decreased (P<0.05), and the observation group was significantly lower than the control group (P<0.05); two groups of Scr, BUN, UA were significantly decreased (P<0.05), and the observation group was significantly lower than the control group (P<0.05); the two groups after treatment IL-6, CRP, TNF-α levels decreased significantly (P<0.05), and the observation group was significantly lower than the control group (P<0.05). Conclusion Shenfu Decoction combined with western standard clinical effect in treatment of elderly MOF is considerable.

Clinical observation on supplemented shenfu decoction combined with western medicine in the treatment of multiple organ failure in the elderly / 中国生化药物杂志

Objective To explore the effect of Shenfu Decoction combined with western medicine treatment of elderly patients with multiple organ dysfunction syndrome (MOF) clinical effect. Methods 65 cases of elderly patients with MOF as the research object, were randomly divided into observation group (n=35) and control group (n=30). The two groups were given conventional standard western medicine treatment, the observation group on the based on the combination of Shenfu Decoction treatment. Results After treatment, two groups of blood pressure and urine volume were significantly increased (P<0.05), and the observation group was significantly higher than the control group (P<0.05); the two groups of patients with respiratory and heart rate were significantly decreased (P<0.05), and the observation group was significantly lower than the control group (P<0.05); two groups of Scr, BUN, UA were significantly decreased (P<0.05), and the observation group was significantly lower than the control group (P<0.05); the two groups after treatment IL-6, CRP, TNF-α levels decreased significantly (P<0.05), and the observation group was significantly lower than the control group (P<0.05). Conclusion Shenfu Decoction combined with western standard clinical effect in treatment of elderly MOF is considerable.

Clinical application of invasive ventilation in treatment of acute pulmonary edema and prognosis analysis / 中华急诊医学杂志

Objective To observe the efficacy of invasive ventilation (Ⅳ) in treatment of acute pulmonary edema (APE), and to explore the factors associated with prognosis. Method From March 2005 to December 2007, 23 APE patients, who were hospitalized in the EICU of People' s Hospital of Peking University and the con-ventional treatment and noninvasive ventilation were not effective, were treated by Ⅳ (PEEP 5～18 cmH2O). Blood pressure, heart rate, respiratory rate, and arterial blood gas values were recorded accurately before and after ventilation and compared with each other. Regression analysis was used to analyze the factors associated with prog-nosis. Resets Three hours after Ⅳ, the blood pressure, heart rate, respiratory rate, arterial blood gas were sig-nificanfly improved (P <0.01). Among the 23 patients, 11 survived, and the other 12 patients died. Nine pa-tients died of MOF. Among 16 patients with cardiac function Ⅲ-Ⅳ, 10 died. Among 15 patients with acute my-ocardial infarction, 9 died. Among 11 patients with renal insufficiency, 9 died. The multivariate logistic regression analysis showed that the reduced left ventricular ejection fraction, the lower mean arterial pressure, and the in-creased creatinine were the independent predictor of prognosis. Conclusions Invasive ventilation is an effective method of treating patients with acute pulmonary edema. Proper ventilation may improve the cardiac function and clinical symptoms, but it's not the fundamental measure for increasing cure rate. 1he renal insufficiency and heart failure are the independent predictor of prognosis.

The APACHE III Score and Multiple Organ Failure(MOF) Score in Patients who were Recipients of Decision-Making Do-Not- Resuscitate / 성인간호학회지

PURPOSE: The purpose of this study was to identify characteristics of patients who were recipients of decision-making DNR, to describe the situations of DNR, and to analyze the APACHE III and MOF scores. METHOD: Data collection was conducted through reviews of medical records of 51 patients and through interviews with families of patients who were decision-makers for DNR at C university K Hospital located in Seoul from April to September 2002. RESULTS: The men's APACHE III and MOF scores were higher than the women's and the non cancer patients were higher than cancer patients. Some 80.4% of DNR orders was by communication, while 11.8% of consents were written. Each of APACHE III and MOF scores of patients in the intensive care unit was higher than the patients in general ward at both points of admission and decision-making of DNR. APACHE III and MOF scores positively correlated statistically with each other. CONCLUSIONS: The findings of this study suggest that APACHE III and MOF scores be useful for decision-making of DNR as a tool measuring severity.

Indications and Limitations of IABP Support for Acute Cardiac Failure after Artificial Valve Replacement / 日本心臓血管外科学会雑誌

We evaluated the indications and limitations of IABP support for weaning for pump and postoperative heart failure after artificial valve replacement. Driving IABP as cardiac support in cases of acute cardiac failure during and after operation, it is most effective for transient cardiac failure-associated coronary artery spasms during and post operation, but it is not effective and is indeed limited for patients who need long term extracorporeal circulation because of operative technical failure, insufficient cardioplegia and delayed right ventricular failure after operation. The latter groups, in which IABP is insufficiently effective need additional assist devices such as V-A bypass and ventricular assist device (VAD).

ADVANCED MALIGNANT TUMORS OF THE AGED AND MOF / 西安交通大学学报(医学版)

23 cases of advanced malignant tumors of the aged and MOFare analysed It has been found that the sequences of MOF are lung, kidney, heart, liver, brain, gastro-intestine ard Dic, amounting to the average of 3.4 organs with a death rate of 100%. The results indicate that old people with advanced malignant tumors have very low immunity. The attack of organs by the primary and secondary tumors is the basic factor of MOF, and various infections are its precipitating factor. The incidence characteristics, prevention and treatment of the MOF are discussed.