Simultaneous Determination of 5 Saponins in Lonicerae Flos by QAMS Method / 中国药房

OBJECTIVE:To establish the method for simultaneous determination of 5 saponins in Lonicerae Flos. METHODS:Using macranthoidin B as a reference,HPLC method was adopted to calculate the relative correction factor(RCF)of it to macran-thoidin A,dipsacoside B,macranthoside A and macranthoside B. The contents of above 4 saponins were calculated through RCF. Using the contents of saponins determined by external standard method as measured value,the calculated value was compared with measured value. RESULTS:The linear ranges of macranthoidin A,macranthoidin B,dipsacoside B,macranthoside A and macran-thoside B were 0.316-6.32 μg(r=0.9973),0.453-9.06 μg(r=0.9982),0.231-4.62 μg(r=0.9996),0.342-6.84 μg(r=0.9984) and 0.147-2.94 μg(r=0.9961),respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 97.74%-104.51%(RSD=2.37%,n=6)、96.70%-103.20%(RSD=2.37%,n=6)、96.12%-103.61%(RSD=2.45%, n=6)、98.80%-104.70%(RSD=2.32%,n=6)、99.21%-102.92%(RSD=1.39%,n=6),respectively. There was no statistical sig-nificance between calculated value and measured value(P>0.05). CONCLUSIONS:The method is simple,precise,stable and re-producible. It can be used for the determination of saponins in Lonicerae Flos.

Chemical constituents from caulis of Lonicera dasystyla / 中草药

Objective: To search for the bioactive compounds from the vine of Lonicera dasystyla. Methods: The compounds were isolated and purified by column chromatography on silica gel, sephadex LH-20 gel, and ODS, and their structures were elucidated on the basis of spectroscopic analysis, such as MS, NMR, etc. Results: Nine compounds were isolated from the vine of L. dasystyla. Their structures were identified as dihydrosesamin-9-O-β-D-glucopyranoside (1), isoferulic acid (2), p-methoxycoumaric acid (3), kaempferol-7-O-(2″-E-p-coumaroyl)-α-L-arabinofuranoside (4), macranthoidin B (5), loganin (6), kaempferol (7), acacetin (8), and quercetin (9). Conclusion: All of these compounds belong to four types, phenylpropanoids (1-3), flavonoids (4, 7-9), triterpenoid saponin (5), and iridoid (6). Compound 1 is a new one and compounds 5 and 6 as marker compounds exist in most of Lonicera Linn. plants, while known compounds 2-4 and 8 were isolated from Lonicera Linn. plants for the first time. The identification of these chemicals lays the foundation for searching bioactive compounds of this plant, and these compounds have great importance for chemotaxonomy of this plant.

Identification and attribution of Lonicerae Japonicae Flos and Lonicera Flos / 中草药

Objective: To identify Lonicerae Japonicae Flos and Lonicera Flos with various methods, using Lonicera japonica to represent Lonicera Flos for searching the different characteristics and discussing their attribution. Methods: An identification method on the basis of biological character comparison, structure observation of the flower epidermal by using SEM, and difference comparison of the marked components (chlorogenic acid, galu teolin, macranthoidin B, dipsacoside B) was established. The attribution of the two herbs on the account of Chinese Pharmacopoeia was discussed according to the historic using status and the present market situation. Results: In L. japonica, plenty of glandular hairs with turbinate top in flower epidermis and large leafy bracts in the bottom of flower were observed. Macranthoidin B and dipsacoside B were not detected. But in L. macranthoides, there were few leafy bracts and glandular hairs, and macranthoidin B and dipsacoside B were detected. Conclusion: Leafy bracts in the bottom of flower, glandular hairs in flower epidermis, and two characteristic components (macranthoidin B and dipsacoside B) could be used as basis for the identification of L. japonica and L. macranthoides.