Secondary metabolites from Colletotrichum fioriniae F18, an endophytic fungus isolated from the medicinal plant Mahonia fortunei / 药学学报

Chemical investigation on the rice culture of an endophytic fungus Colletotrichum fioriniae F18, inhabiting in the stems of the medicinal plant Mahonia fortunei, led to the isolation of nine compounds. They included a new indole alkaloid, makomotindoline B (1), and two known indole derivatives, 3-indoleacetic acid methyl ester (2) and N-acetyltryptamine (3), together with six known aromatic compounds, 2-(4-hydroxyphenyl) acetic acid (4), 4-(2-hydroxyethyl)phenol (5), 2-(4-methoxyphenyl)acetic acid (6), 4-hydroxyphenethyl 2-(4-hydroxyphenyl)acetate (7), regiolone (8) and N-phenethylacetamide (9). The structures of these compounds were elucidated based on the analysis of spectroscopic data including MS and NMR. The absolute configuretion of compound 1 was determined by electronic circular dichroism (ECD) calculation. Antibacterial activity assay indicated that compounds 1-9 had no antibacterial activities against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, as well as no quorum sensing inhibitory (QSI) activity for Chromobacterium violaceum.

Chemical constituents of lipophilic parts in water extrct from stem of Mahonia fortunei / 中草药

Objective To study the chemical constituents of lipophilic parts in water extracts from the stem of Mahonia fortunei. Methods The compounds were isolated and purified by repeated column chromatography on silica gel and high performance liquid chromatography, and their structures were determined by spectroscopic data analysis. Results Thirty-eight compounds were obtained from lipophilic parts in water extracts from the stem of M. fortunei and identified as maltol (1), 3-hydroxy-4-methoxyphenylethanol (2), syringylethanone (3), 1-hydroxy-3-(4-hydroxy-3-methoxyphenyl)-2-propanone (4), α-hydroxypropiosyringone (5), corydaldine (6), zhebeiresinol (7), vanillic acid (8), springic acid (9), noroxyhydrastinine (10), (+)-syringaresinol (11), episyringaresinol (12), schisandrin (13), neoechinulin A (14), 8-oxyjatrorrhizine (15), 8-oxyberberine (16), 5-hydroxymaltol (17), methyl-5- hydroxy-2-pyridinecarboxylate (18), vomifoliol (19), 2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (20), oleracein E (21), acortatarin A (22), 5-epi-acortatarin A (23), 5-(methoxymethyl)-1H-pyrrole-2-carbaldehyde (24), (+)-lyoniresinol (25), (−)-secoisolariciresinol (26), ciwujiatone (27), protocatechuic acid (28), adenosine (29), 3,4,5-trimethoxyphenyl-1-O-β-D- glucopyranoside (30), 2-(4-hydroxy-3-methoxyphenyl) ethyl-O-β-D-glucopyranoside (31), tortoside B (32), oldhamioside (33), (+)-syringaresinol-4′-O-β-D-glucopyranoside (34), episyringaresinol-4′-O-β-D-glucopyranoside (35), berberine (36), 3,4,5-trimethoxyphenyl-(6′-O-syringoyl)-O-β-D-glucopyranoside (37), and salidroside (38), respectively. Conclusion Compounds 3—7, 13—15, 17—19, 21—27, 29, 31, 33, and 38, are isolated from the genus Mahonia for the first time. Moreover, the nuclear magnetic resonance data of compound 15 is assigned for the first time.