The protective effect of MANF on intestinal epithelial cell injury induced by endoplasmic reticulum stress / 中国药理学通报

Aim To observe the proteetive effect of meseneephalie astrocyte-de rived neurotrophic faetor (MANF) on intestinal epithelial eells under endoplas- mic reticulum stress (EH stress).Methods Normal human intestinal epithelial cell line FHs74Int was stimulated with EH stress inducers, tunicmycin (TM) and TNF-cx, then the expression of endogenous MANF was observed.The recombinant plasmids MANF-GFP and GFP were transfected into FHs74Int cells individually, the transfection efficiency was observed by fluorescence j j microscopy, and the effect of MANF on EH stress was observed by Western blot.The effect of MANF on the proliferation of intestinal epithelial cells stimulated by TM was detected via CCK-8 assay.The effect of MANF on apoptosis after EH stress was detected by Western blot and flow cytometry.Results EH stress could induce the expression of endogenous MANE in intestinal epithelial cells.Overexpression of MANE significantly inhibited the expression of the EH stress-related proteins, Bip and CHOP, and promoted the proliferation of intestinal epithelial cells.At the same time, it could reduce the production of the proapoptotic proteins cleaved-c a spa se-3 and Bax, increase the expression of the antiapoptotic protein Bcl-2, and inhibit the proportion of early and late apoptosis of intestinal epithelial cells.Conclusions MANF plays a protective role in inhibiting EH stress in intestinal epithelial cells by promoting cell proliferation and reducing apoptosis.

Risk assessment of knee joint injury induced by simplified 24-action Taiji Quan wild horse mane splitting practice / 中国康复理论与实践

ObjectiveTo investigate the injury risk of simplified 24-action Taiji Quan wild horse mane splitting practice at different postures with mechanical analysis. MethodsThe mechanical model of the action of Taiji Quan wild horse mane splitting was establishied, and then the coordinates, velocity, acceleration and horizontal angle of thigh and lower leg were determined by image analysis. The force arm of quadriceps femoris was detected by X-ray, and the quadriceps femoris tension, femoral tibial joint force and patellofemoral joint pressure of wild horse mane splitting practice with different postures were compared. ResultsCompared with the high posture, the coordination and velocity decreased, the average angle of knee joint decreased from 128° to 115° at the low posture; and the knee moment, the quadriceps femoris tension, femoral tibial joint force and patellofemoral joint pressure significantly increased (F > 37.187, P < 0.001). ConclusionWhen practicing Taiji Quan wild horse mane splitting, the risk of knee injury at the low posture is higher than that at high posture.

Paciente con síndrome de Eisenmenger complicado por sangrado digestivo alto / Patient with Eisenmenger syndrome complicated by upper digestive bleeding

El Síndrome de Eisenmenger es un desorden multisistémico caracterizado por hipertensión pulmonar con inversión o bidireccionalidad del flujo a través de una comunicación intracardiaca o aortopulmonar. Se presenta el caso de un paciente masculino de 48 años de edad con antece- dentes de Hipertensión Pulmonar e Insuficiencia Cardíaca en tratamiento, quien acude por cuadro de melenas, decaimiento e inestabilidad hemodinámica. Al examen físico se ausculta soplo sistólico ascendente en foco pulmonar, abdomen distendido con presencia de onda ascíti- ca. En los exámenes complementarios; la radiografía convencional de tórax mostró aumento de la trama vascular pulmonar, además de encontrarse alteraciones electrocardiográficas. El paciente mostró una evolución desfavorable produciéndose falla cardíaca, que dio lugar al posterior fallecimiento debido a un paro cardiorrespiratorio.

Eisenmenger Syndrome is a multisystemic disorder identified by pulmonary hypertension with reversal or bidirectional flow through intracardiac or aortopulmonary communication. The case of a 48-year-old male patient with a history of Pulmonary Hypertension and Heart Failure in treatment is presented, who comes due to mane of mane, decay, and hemodynamic instability. The physical examination auscultated systolic murmur ascending in pulmonary focus, distended abdomen with presence of ascites wave. In the complementary exams, conventional chest x-ray, increased pulmonary vascular weft, in addition to finding electrocardiographic abnormalities. The patient seemed an unfavorable evolution, producing a heart failure, which resulted in subse- quent death due to cardiorespiratory arrest.

Sangrado digestivo alto como complicación en un paciente lúpico con síndrome de Eisenmenger / High digestive bleeding as a complication in a lupus patient with Eisenmenger syndrome

El síndrome de Eisenmenger es un trastorno multisistémico caracterizado por hipertensión pulmonar con inversión o bidireccionalidad del flujo a través de una comunicación intracardiaca o aortopulmonar. El propósito de este estudio es dar a conocer las características generales del síndrome de Eisenmenger. Se presenta el caso de un paciente masculino de 48 años de edad con diagnóstico de lupus eritematoso sistémico de 6 años de evolución con hipertensión pulmonar e insuficiencia cardiaca en tratamiento, que acude por cuadro de melenas, decaimiento e inestabilidad hemodinámica. Al examen físico destaca la presencia de un abdomen distendido y edemas de los miembros inferiores. Se realizaron exámenes complementarios hasta llegar al diagnóstico de síndrome de Eisenmenger. A pesar de presentar múltiples complicaciones en el curso del lupus eritematoso sistémico no se encontraron reportes que asociaran al lupus con el síndrome de Eisenmenger. El diagnóstico en la infancia de las cardiopatías congénitas cianóticas y la realización de la cirugía correctora constituyen la principal medida preventiva de aparición del síndrome de Eisenmeger(AU)

Eisenmenger syndrome is a multisystemic disorder characterized by pulmonary hypertension with inversion or bidirectional flow through intracardiac or aortopulmonary communication. To publicize the general characteristics of Eisenmenger syndrome. The case of a 48-year-old male patient with a diagnosis of 6-year-old systemic lupus erythematosus with pulmonary hypertension and cardiac insufficiency in treatment is presented, which is due to mane of mane, decay and hemodynamic instability. The physical examination highlights the presence of a distended abdomen and edema of the lower limbs. Complementary examinations were made to reach the diagnosis of Eisenmenger syndrome. Despite presenting multiple complications in the course of systemic lupus erythematosus, no reports were found that associated lupus with Eisenmenger syndrome. Diagnosis of cyanotic congenital heart disease in childhood and corrective surgery are the main preventive measures for the appearance of Eisenmenger syndrome(AU)