Chemoterapeutic drug screening based on patient?derived pancreatic cancer xenograft(PDX)models / 中国实验动物学报

Objective To evaluate the therapeutic effect of chemotherapeutic drugs on pancreatic carcinoma based on patient-derived xenograft(PDX)models,and to screen an individualized treatment strategy. Methods Fresh human pancreatic carcinoma tissues were subcutaneously transplanted into nude mice to establish PDX models which could be stab-ly passaged. The traceability of PDX models was determined by STR analysis. The PDX models were treated with three dif-ferent clinical chemotherapeutic drugs oxaliplatin, gemcitabine and irinotecan, respectively, and the tumor volumes were measured at different times. The therapeutic effect of those drugs was assessed by TGD mathematical model and plasma CA19-9 test. Results The traceability of patient-derived xenograft samples was up to 99.99%. Compared with the con-trol group,the treatment with irinotecan and gemcitabine inhibited tumor growth significantly(P=0.001), and gemcit-abine had even better result. The minimum toxic effect in the mice was induced by irinotecan treatment,followed by gem-citabine treatment. Conclusions Pancreatic carcinoma PDX models are successfully established and can be stably pas-saged. Gemcitabine shows the most inhibitory effect on tumor growth based on TGD mathematical model assessment, and deserves to be recommended as the preferred drug for individual treatment of pancreatic carcinoma.

Influência do modelo alométrico na relação entre consumo máximo de oxigênio e desempenho de corredores fundistas / Influence of the allometric model on relationship between running economy and performance in long-distance runners / Influencia del modelo alométrico en la relación entre consumo máximo de oxígeno y rendimiento en corredores fondistas

Resumo Investigou-se o efeito do modelo alométrico na relação entre o consumo máximo de oxigênio (VO2máx) e o desempenho em corrida de longa distância. Doze corredores fundistas do sexo masculino (idade: 28,6 ± 7,4 anos; massa corporal: 67,9 ± 9,4 kg; estatura: 1,71 ± 0,7 m) foram submetidos a um teste de esforço máximo em esteira rolante para a determinação do VO2máx e a uma corrida máxima de 10.000 m. Apesar das diferenças significativas verificadas entre as formas de relativização do VO2máx (pela massa corporal total, pelo expoente alométrico amostral e pela massa corporal magra), as fortes correlações verificadas entre VO2máx e desempenho demonstram que a predição desse independe da forma de relativização da potência metabólica máxima.

Abstract The aim was investigated the effect of allometric model in relationship between maximal Oxygen uptake (VO2max ) and long-distance running performance. Twelve runners (age: 28.6 ± 7.4 years, body mass: 67.9 ± 9.4 kg, height: 1.71 ± 0.7 m) were submitted to an incremental treadmill running protocol for determination of VO 2max and participated in a trail of 10.000 m. despite the significant differences found between the forms of relativization of VO2max (by total body mass, allometric exponent for sampling and lean body mass), the strong correlations verified between VO2max and performance show that this prediction is independent of how the VO2max is relativized.

Resumen Se investigó el efecto del modelo alométrico en la relación entre el consumo máximo de oxígeno (VO2máx ) y el rendimiento en carreras de larga distancia. Doce corredores fondistas de sexo masculino (edad: 28,6 ± 7,4 años; masa corporal: 67,9 ± 9,4 kg; estatura: 1,71 ± 0,7 m) pasaron un test de esfuerzo máximo en una cinta de correr para la determinación del VO2máx y participaron en una carrera de 10.000 m. A pesar de las diferencias entre las formas de relativización del VO2máx (por la masa corporal total, por el exponente alométrico general de la muestra y por la masa corporal magra), las fuertes correlaciones encontradas entre VO2máx y rendimiento demuestran que la predicción de éste es independiente de la forma de relativización del consumo máximo de oxígeno.

Establishment of Mathematic Model of the Extraction Kinetics of the Components of Chinese Complex Prescription and the Effect of Particle Size on the Concentration of Catalpol in Rehmanniae Decoction of Liuwei Dihuang Decoction / 中国药房

OBJECTIVE:To establish a mathematical model for the dissolution kinetics of the components of the closed diffusion system of Chinese complex prescription,and to study and analyze the parameters of the extraction kinetics of catalpol,an active component in rehmanniae decoction of Liuwei dihuang decoction. METHDOS:The mathematic modeling method was applied to the extraction process of the traditional Chinese medicine; the mathematic model of the extraction process of the Chinese medicine was established based on the extraction principle and Fick law,and the function expression was derived from the fitting of the mathematic model and analysis of parameters,then the model was applied to the study of the kinetic parameters of the catalpol in rehmanniae decoction of Liuwei dihuang decoction. RESULTS:The analytic solution of the dissolution concentration of components consisting of 3 e in exponential form and the analytical method of parameters were obtained in this study. The optimal grain size of the herbal medicine was 60 meshes.Under this condition,the maximal peak time(tmax)of catalpol was 195.1 min;the peak concentration(Cmax) was 0.012 23 mg?mL-1;the maximal extraction rate(P) was 72.95%,and the loss rate(D) was 7.62%. CONCLUSION:The dissolution of the components of the closed soluble diffusion system of Chinese complex prescription fitted linear kinetic mathematical model,and the parameters can be derived from the expression relation of the dissolution concentration.

Study on the epiemiological patterns of HIV infection in HongKong by a mathematic model / 中国艾滋病性病

Objective To study the epidemiological patterns of HIV infection prevalent in HongKong.Method The epidemiological patterns of HIV infection in HongKong were analyzed with a mathematic model SI by changing its initial condition,which was used to test the related data.Results The changeable patterns of susceptible accumulative numbers of HIV infection in HongKong and the epidemiological curves and the curve of infection velocity were obtained.Conclusion The epidemic pattern of HIV infection in Hongkong and local infection velocity from 1987 to 2004 were well described using the epidemic model SI.An accumulation of 2 733 cases of HIV infection will be predicted in the year of 2005.The epidemic peak occurred in 2002 to 2004 and the overall HIV epidemic will last for 50 years approximately.

Simulation study of canceling technology for strong clutters in life detector based on radar / 医疗卫生装备

Strong clutters are produced by the echo waves from the debris when the life detector based on radar locates the living human subject under the debris, which makes the life detector work abnormally because the amplifier is saturate. A canceling technology for the strong clutters is provided to solve the problems discussed above in this paper. The canceling technology for the strong clutters can provide methods and theories in practice by constructing and simulating the mathematic model in the computer, analyzing and discussing the results.

Establishment of Mathematic Model of the Correlativity of HCMV Infection Between Mothers and Their Newborns / 中国医师杂志

Objective To establish a mathematic model of correlativity of human cytomegalovirus(HCMV) infection between mothers and their newborns.Method The plasma HCMV-IgM content,HCMV-DNA levels in plasm and peripheral blood mononuclear cells (PBMCs) were assayed with ELISA and PCR technique.Results There was a evident correlation between the two generation,and there was largest correlativity between HCMV-DNA level of mother's plasma and HCMV congenital infection rate,its spearman rank correlative coefficient was 0 7018(P

Pharmacokinetics and Pharmacodynamics of Intravenous Famotidine in Healthy Volunteers / 第二军医大学学报

The pharmacokinetics and pharmacodynamics of famotidine were investigated in 10 healthy male volunteers after single intravenous administration of 20 mg. The blood drug levels were determined by a high performance liquid chromatography. The 1gC of famotidine in plasma vs time curve were found to be twcncompartment open model in healthy volunteers. The terminal half-life averaged 3.16h; the total distribution volume 99.40L; the total plasma clearance 392.12ml/min; the area under the plasma concentration curve 1057.45 h?ng/ml. A mathematic equation describing the whole course of blood drug levels in relation to inhibitory effects on intragastric acid output is as follows: E= 100?C2.60/(C2.60+14.712.60). The constant 14.71 is EC50 (ng/ml), the blood drug concentration producing 50% of maximal pharmacological effects. Prediction of pharmacodynamic effects from blood drug level and vice versa becomes possible by using the mathematic equation.