RESUMO
Objective Intrauterine adhesions cause serious damage to women′s reproductive health, to establish animal dis?ease mode, pure mechanical damage is the most similar cause to human pathogenesis, that is very necessary to conduct prospective studies. The study aimed to investigate the efficiency and significance of different mechanical damage methods to establish intrauterine adhesions model in rats. Methods 45 female SD rats were randomly( random number table) divided into three groups:pure scratch group (n=15), 2 mm diameter curet was used to scrape endometrial tissue; Incision?suture group (n=15), a longitudinal incision was made in the uterus, a blade was used to scrape endometrial tissue, the incision was then sutured with absorbable thread;Control group ( n=15) , sham operation, then the abdomen was closed after exposed to air for 20 minutes, the uterus were not injured. Endometri?al keratin immunohistochemical staining, Intrauterine AFS score, fi?brosis semi?quantitative score and pregnancy outcomes were observed to compare the injury of the two methods in establishing the model. Results ( 1) The degree of endometrial glandular epithelium keratin staining decreased significantly in both pure scratch and incision?su?ture groups compared to the control group. (2) AFS scores in pure scratch group and incision?suture group[3.0 (2.0-3.8), 5.0 (3.8-8.0)] were higher than control group[0 (0-0)], the difference was statistically significant (P0.017). Endometrial fibrosis semi?quantitative scores in pure scratch group and incision?suture group[5.0 (4.0-5.3), 6.5 (5.8-8.0)] were higher than control group[0 (0-0)], the difference was statistically significant ( P0.017). (3) Embryo number in pure scratch and incision?suture group side [0.5 (0-3.3), 0 (0-2.3)] was lower than the normal side [7.6 (6.8-8.0), 8.0 (7.8-9.0)],the difference was statistically significant (P<0.05) Conclusion Pure mechanical damage including pure scratch and incision?suture method to establish IUAs model were feasible. Both of the two methods could help to do research about pathogenesis and pathophysiological mechanisms of IUAs. Incision?suture method maybe can cause heavier fibrosis degree.
RESUMO
A colheita mecanizada de soja pode acarretar perdas qualitativas nas sementes. O presente trabalho teve como objetivo avaliar a qualidade de sementes de soja colhidas mecanicamente por sistemas axial e tangencial de trilha, em diferentes velocidades de avanço da colhedora, antes e após o período de armazenamento de seis meses. Utilizou-se o delineamento experimental de blocos casualizados, em esquema de parcela subdividida no tempo, com quatro repetições. Nas parcelas, foram avaliados os procedimentos de colheita e, nas subparcelas, as épocas de avaliação da qualidade da semente. Os procedimentos de colheita foram: colhedora com sistema de trilha axial, deslocando-se a 6, 8 e 10km h-1, colhedora com sistema de trilha tangencial (convencional), deslocando-se a 4 e 6km h-1, e colheita manual. Foram analisadas as seguintes variáveis: germinação, porcentagem de plântulas fortes, índice de velocidade de emergência (IVE), emergência em areia e injúria mecânica. Pôde-se concluir que o emprego das colhedoras com sistemas de trilha tangencial e axial não provocou diferenças no índice de velocidade de emergência, no vigor e na germinação das sementes de soja. No entanto, com relação à injúria mecânica, a colhedora axial mostrou-se superior à convencional. O incremento da velocidade de deslocamento, dentro dos parâmetros recomendados pelo fabricante, não alterou a qualidade das sementes. O armazenamento reduziu o vigor das sementes colhidas.
Soybean mechanical harvest may bring qualitative losses to seeds. This study aimed to evaluate the quality of soybean seeds mechanically harvested by axial and conventional threshing systems, at different displacement speeds of the harvesters, before and after the storage period of six months. A randomized complete block design in a split-plot arrangement with four replications was used. The main plots were the harvest systems and the split-plots were the storage times. The harvest systems were: axial flow harvester, at 6, 8 and 10km h-1, conventional harvester, at 4 and 6km h-1, and manual harvest. Germination, percentage of normal seedlings, emergency speed index, sand emergency and mechanical damage of soybean seeds were evaluated. The results showed that the use of axial flow and conventional harvesters did not affect the emergency speed index, vigor and germination of soybean seeds. However, the axial flow system provided less mechanical damage. The increase of displacement speed, within the ranges of the operating parameters recommended by the manufacturer, did not change seed quality. Storage reduced seed vigor.