High performance liquid chromatography combined with microwave-assisted extraction and solid phase extraction in determination of mefenacet residues in rice / 第二军医大学学报

Objective: To establish a method by combining microwave-assisted extraction (MAE), solid phase extraction (SPE) and high performance liquid chromatography (HPLC) for determination of the mefenacet residues in rice. Methods: Acetone and acetontrile (3:7) were used as extraction solvent. Microwave-assisted extraction was used to extract mefenacet residues in the rice. The extracts were then cleaned up with a Florisil cartridge and then subjected to Hypersil C18 column(5 μm,4. 6 mm × 200 mm), with acetonitrile: water(50: 50,V/V)solution as mobile phase and with a flow rate of 1.0 ml/min: the ultraviolet detection wavelength was at 217 nm. Results: Good linear correlation for mefenacet was found within a concentration range of 0. 198-9. 900 μg/ml. The detection limit was 0. 039 6 μg/mL for mefenacet(S/N=2). The average recovery rate of rice hull and brown rice were 90.8% (RSD 1. 8%) and 85. 6% (RSD 2. 5%),respectively. Conclusion: The present method is simple and rapid: it can be used for the determination of mefenacet residues in rice.

High performance liquid chromatography combined with microwave-assisted extraction and solid phase extraction in determination of mefenacet residues in rice / 第二军医大学学报

Objective:To establish a method by combining microwave-assisted extraction(MAE),solid phase extraction(SPE)and high performance liquid chromatography(HPLC)for determination of the mefenacet residues in rice.Methods:Acetone and acetontrile(37)were used as extraction solvent.Microwave-assisted extraction was used to extract mefenacet residues in the rice.The extracts were then cleaned up with a Florisil cartridge and then subjected to Hypersil C18 column(5 ?m,4.6 mm?200 mm),with acetonitrilewater(5050,V/V)solution as mobile phase and with a flow rate of 1.0 ml/min;the ultraviolet detection wavelength was at 217 nm.Results:Good linear correlation for mefenacet was found within a concentration range of 0.198-9.900 ?g/ml.The detection limit was 0.039 6 ?g/mL for mefenacet(S/N=2).The average recovery rate of rice hull and brown rice were 90.8%(RSD 1.8%)and 85.6%(RSD 2.5%),respectively.Conclusion:The present method is simple and rapid;it can be used for the determination of mefenacet residues in rice.