Chemical constituents from n-butanol parts of Lycii Cortex / 中草药

Objective: To study the chemical constituents from n-butanol-souluble part of Lycii Cortex (the root bark of Lycium chinense). Methods: The air-dried Lycii Cortex were powdered and extracted with 70% ethanol under reflux. After the removal of solvent under reduced pressure, the crude extract was extracted with petroleum ether, ethyl acetate and n-butanol successively. The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS and semi-prepared high performance liquid chromatography from the n-butanol part of Lycii Cortex. The structures were identified by nuclear magnetic spectrometry, mass spectrometry and other spectral analyses. Results: Ten compounds were isolated from n-butanol parts of Lycii Cortex and characterized as (1'S,2R,5S,10R)-2-(1',2'-dihydroxy-1'-methylethyl)-6,10-dimethylspiro [4,5] dec-6-en-8-one 2'-O-β-D- glucopyranoside (1), (1'R,2R,5S,10R)-2-(1',2'-dihydroxy-1'-methylethyl)-6,10-dimethylspiro [4,5] dec-6-en-8-one 2'-O-β-D- glucopyranoside (2), (1R,6R,9S)-6,9,11-trihydroxy-4,7-megastigmadien-3-one 11-O-β-D-glucopyranoside (3), vanillic acid-4-O-β- D-glucopyranoside (4), 3,4-dihydroxyphenylpropionic acid (5), 3,4-dihydroxybenzenepropionic acid methyl ester (6), glucosyringic acid (7), dihydrophaseic acid 3'-O-β-D-glucopyranoside (8), isoscoploletin-β-D-glucoside (9) and fabiatrin (10). Conclusion: Compound 3 is isolated from Solanaceae family for the first time and compounds 1, 2 and 4 are isolated from Lycium genus for the first time. The NMR data of compound 2 is first reported as well.

Chemical constituents from the aerial parts of saururus chinensis() / 中国药学杂志

OBJECTIVE: To study the chemical constituents from the aerial parts of Saururus chinensis. METHODS: The compounds were extracted by supercritical CO2 extraction and isolated by various chromatographic methods, such as silica gel, MCI and pre-HPLC. The structures were elucidated by physico-chemical constants and spectroscopic methods. RESULTS: Fourteen compounds were isolated and identified as N-p-trans-coumaroyltyramine (1), linarin (2), blumenol A (3), mannitol (4), 5,7-dyhydroxyl-3,4′-bimethoxyflavonoids (5), 6,7-dimethoxy-4-hydroxy-1-naphthalene formic acid (6)3-hydroxy-4-methoxy benzoic acid (7), (2R,3R)-2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-7-methoxy-3-methylbenzofuran-5-aldehyde (8), (6S,7E)-6-hydroxy-4,7-megastigmadien-3,9-dione (9), veratric acid (10), p-hydroxybenzaldehyde (11), syringaldehyde (12), 3,4,5-trimethoxybenzoic acid (13) and physcion (14). CONCLUSION: Compounds 1-13 are isolated from this plant for the first time.

Chemical Constituents from Caulophyllum robustum (Ⅱ) / 中国药学杂志

OBJECTIVE: To isolate the chemical constituents from Caulophyllum robustum and confirm their chemical structures. METHODS: The chemical constituents were isolated by MCI gel, repeated silica gel chromatography, preparative liquid chromatography.and their structures were elucidated by NMR and MS etc. RESULTS: The structures of compounds 1-10 were identified as echinocystic acid (1), oleanolic acid-3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranoside (2), hederagenin-3-O-β-D-glucopyranosyl-(1→3)-α-L-arabinopyranoside (3), hederagenin-3-O-β-D-glucopyranosyl-(1→2) [β-D-glucopyranosyl-(1→3)]-α-L-arabinopyranoside (4), 3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl echinocystic acid-28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (5), 3-O-α-L-arabinopyranosyl hederagenin-28-O-(4-O-acetyl)-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (6), (6R, 7E, 9R)-9-hydroxy-4, 7-megastigmadien-3-one-9-O-β-D-glucoside (7), (9R)-9-hydroxy-4, 6-megastigmadien-3-one-9-O-β-D-glucoside (8), maltose (9), and sucrose (10). CONCLUSION: Compounds 1-10 are firstly isolated from the genus Caulophyllum except 5.

Chemical constituents from seeds of Cuminum cyminum / 中草药

Objective To investigate the chemical constituents from the seeds of Cuminum cyminum. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography, high pressure flash chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis. Results Fifteen compounds were identified as astragaline (1), 6-β-hydroxy glycyrrhetinic acid (2), ursolic acid (3), quercetin (4), protocatechuic acid (5), (6R,7E,9R)-9-hydroxy-4,7- megastigma-dien-3-one (6), 4,5-dicaffeoylquinic acid methyl ester (7), 3,5-dicaffeoylquinic acid methyl ester (8), rosin (9), kaempferol 3-O-β-D-glucopyranosyl (1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (10), cuminacid (11), cuminoside A (12), luteolin (13), apigenin-7-O-β-D-(6″-O-acetyl)-glucopyranoside (14), and 5,7,4’-trihydroxyflavanone (15). Conclusion Compounds 2, 3, 6-10, and 14 are obtained from this genus for the first time.

3-Hydroxy-4,7-megastigmadien-9-one, Isolated from Ulva pertusa Kjellman, Inhibits LPS-Induced Inflammatory Response by Down-Regulating Mitogen-Activated Protein Kinase and NF-κB Pathways

In the present study we evaluated the anti-inflammatory potential of 3-hydroxy-4,7-megastigmadien-9-one (Comp) isolated from Ulva pertusa Kjellman, in LPS-stimulated bone marrow-derived dendritic cells (BMDCs). Comp treatment exhibited strong dose dependent inhibition of IL-12 p40 and IL-6 cytokine production with IC₅₀ values of 7.85 ± 0.32 and 7.86 ± 0.18, respectively in LPS-stimulated BMDCs. Treatment of Comp inhibited MAPKs and NF-κB pathways in LPS-stimulated BMDCs by inhibiting the phosphorylation of ERK1/2, JNK1/2, p38 and IκB. Thus, these results suggest that Comp have a significant anti-inflammatory property and affirm further studies concerning the potentials of Comp for medicinal use.