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Melastoma dodecandrum is commonly used in the folk. It has been widely used in China by She, Yao, Miao, and other ethnic minorities, which has the effects of blood activating and hemostasis, elimination of swelling and stasis, and heat-clearing and detoxifying. In this review, the relevant researches about M. dodecandrum were summarized from the textual research, resource distribution, standards, pharmacological activity, chemical composition and clinical application aspects, which provides a theoretical basis for the further study and clinical application of M. dodecandrum.
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Objective To develop a HPLC method for the simultaneous content determination of three terpenoids inMelastoma dodecandrum Lour. (asiatic acid, betulinic acid, oleanolic acid).Methods The chromatographic column was set with waters SunFire C18 column (4.6 mm×150 mm, 5μm); the moving phase was acetonitrile -0.1%H3PO4; the column temperature was 30℃; the detection wavelength was 200 nm; the flow rate was 0.6 ml/min; and the sample volume was 25μl.Results A good linear relationship was observed in the range of 0.310-6.200μg (r=0.9999), 0.405-8.100μg (r=0.9999), 0.169-3.375μg (r=0.9998) for asiatic acid, betulinic acid, oleanolic acid respectively, with the average recovery rates of 102.08%, 101.81%, 102.22%. Conclusions The established method is convenient and sensitive, repeatability and stability, quality evaluation for Melastoma dodecandrum Lour.
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Objective To study the constituents in Melastoma dodecandrum.Methods The constituents were isolated by chromatographic methods,and their structures were elucidated by spectroscopic evidences.Results Five compounds were purified and their structures were identified as: daucosterol(Ⅰ),oleanolic acid(Ⅱ),avicularin(Ⅲ),3,7,4′-trimethoxyquercetin(Ⅳ),and atractylenolidone(Ⅴ).Conclusion Compound Ⅴ is a new chemical constituent named atractylenolidone.Compound Ⅳ is isolated from M.dodecandrum for the first time.
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Objective To explore the optimal techniques in separation of phenols from Melastoma dodecandrum with macroporous resins.Methods Static and dynamic adsorption-desorption methods were adopted,and the concentration of phenols was measured by UV spectrophotometer to evaluate the separa-ting efficiency.Results The HPD 100 macroporous resin was found with the best separating efficiency,by which the content of phenols could reach more than 50 percent in the extraction.The optimum technique condition was 0.2 g/mL concentration of crude drug,pH value was 2.7—4.5,adsorption in 3 BV/h,delution by 5 BV,60% alcohol in 3 BV/h.Conclusion This method is simple and feasible with good efficiency of separation,which could also meet the industrial requirements.