THERAPEUTIC POTENTIAL OF AROMATIC PLANT LEAF (MENTHA PIPERITA L.) AND FRUIT EXTRACT (FLACOURTIA JANGOMAS LOUR) AGAINST THE UTI BACTERIAL PATHOGENS

Medicinal plants are considered colossal producers of bioactive therapeutics agents. Fruit is an essential part of the human diet and is of great interest because of its richness in phytochemicals. The present study was chosen the objectives are assessment the antibacterial activity against the dominantly affected seven Urinary Tract Infected (UTI) pathogenic bacterial species plant leaf extracts and fruits such as Mentha piperita (L.), Flacourtia jangomas (Lour) Raeusch. Experimental UTI pathogenic organisms named as Alcaligens sp., Kliebsiella sp., E. coli, Proteus sp., Staphylococcus sp., Pseudomonas sp., Hence the current result has been clearly depicted that the methanol extract of Mentha piperita leaf expressed the potential signicant antibacterial activity against the ve UTI pathogenic organisms such as Alcaligens sp., and Klebsirlla sp. Proteus species and Pseudomonas sp., than other two experimental extracts of benzene and ethanol. From the present result clearly noticed three elucidated compounds are act as an antibacterial bio-compounds such Octadecanoic acid methyl ester, 1, 2, 4, 5-tetramethyl Piperone and α-Propyl tetradecanol present in methanol leaf.extract of M. piperita (L.) plant. Similarly F. jangomas (Lou.) fruit extract (methanol).possessed the antibacterial property biologically effective compounds named as Lavandulol, α-Humulene and Terpineol. Therefore, both experimental plant leaf and fruit extract possessed signicant antibacterial activity against the tested seven UTI clinical pathogenic organisms Furthermore nanoparticle study also denoted both experimental methanol extracts of M. piperita (L.), (leaf) F.jangomas (Lour) expressed the signicant nanoparticle production. It was noticed the range between 21.50 to 41.03nm and 60.52 to 19.22nm. for M. piperita (L.) F. jangomas fruit juice extract respectively. From the current research clearly showed that methanol extract of both experimental samples could be act as a potential traditional plant based medicines for urinary tract infected diseases.

Bioacessibility of phenolic compounds and antioxidant capacity in organic peppermint leaves

ABSTRACT The objective of this study was to analyze the chemical composition and bioaccessibility of phenolic compounds as well as their antioxidant capabilities of organic peppermint leaves after each phase of simulated digestion. Moisture was determined until a constant weight was obtained in an oven at 105 °C; ash was determined after sample calcination in a muffle furnace at 550 °C. The protein concentration was determined by the Macro-Kjeldahl method and lipid content by hot-extraction in a Soxhlet apparatus. Carbohydrates were calculated from differences and energy values based on the Atwater conversion factors. Total phenolic, flavonoid content, and antioxidant activity were determined by spectrophotometry. A four-step procedure was used for in vitro digestion. Organic peppermint was found to contain the following: 78% moisture, 1.7% ash, 1.5% lipids, 0.3% proteins, 17.7% carbohydrates, and a total of 85.5 kcal/100 g. Values of 705 mg GAE/100 g of phenolic, 918 mg QE/100 g of flavonoids, and 58.8 mg/g of vitamin C were also measured. It was discovered that total phenolics had the highest bioaccessible fraction relative to flavonoids; the salivary phase was identified as that with the highest release of these compounds and thus the phase in which peppermint showed significant antioxidant activity (1509 μmol TEAC/100g). This study demonstrated that organic peppermint has a high content of phenolic compounds that can be extracted from the alimentary matrix in the salivary and intestinal phases of the digestive system. Because of the antioxidant activity of these compounds, the use of this aromatic plant as seasonings and spices is relevant.

RESUMEN El objetivo de este estudio fue analizar la composición química de hojas de menta orgánica y la bioaccesibilidad de los compuestos fenólicos así como su capacidad antioxidante. Se determinó la humedad hasta obtener un peso constante en el horno a 105 °C, la ceniza se determinó en un horno de mufla a 550 °C. La concentración de proteína se determinó mediante el método Macro-Kjeldahl y los lípidos mediante extracción en un aparato Soxhlet. Los carbohidratos se calcularon por diferencia y energía basadas en los factores de conversión de Atwater. El contenido fenólico y la actividad antioxidante se determinaron por espectrofotometría. Se usó un procedimiento de cuatro pasos para la digestión in vitro. La menta orgánica contiene 78% de humedad, 1,7% de cenizas, 1,5% de lípidos, 0,3% de proteínas, 17,7% de carbohidratos y 85,5 kcal/100 g. También se midieron valores de 705 mg GAE/100g de fenólico, 918 mg QE/100 g de flavonoides y 58,8 mg/g de vitamina C. Se descubrió que los fenólicos tenían la fracción bioaccesible más alta en relación con los flavonoides; la fase salival se identificó como aquella con la mayor liberación de estos compuestos y, por lo tanto, la menta mostró una actividad antioxidante significativa (1509 μmol de TEAC/100 g). La menta orgánica tiene un alto contenido de fenólicos que se pueden extraer en las fases salival e intestinal. Debido a la actividad antioxidante de estos compuestos, el uso de esta planta como condimentos y especias es relevante.

Evaluación del potencial hepatoprotector de la Mentha piperita L previo a la inducción de hepatotoxicidad con acetaminofen / Evaluation of hepatoprotective potential of Mentha piperita L before induction of hepatotoxicity with acetaminophen

A preclinical pharmacological study was conducted to evaluate the hepatoprotective effect of Mentha piperita L. against paracetamol induced toxicity. Adult male NMRI mice who were administered orally soft plant extracts in doses of 200mg/kg and 400mg/kg, three consecutive days prior to the induction of hepatotoxicity were used. Clinical signs of toxicity, hepatic biochemical parameters and morphological analysis of the liver was evaluated. The biochemical parameters analyzed showed significant differences, but none of the two groups showed a similar untreated control group behavior. No macroscopic changes in the liver were confirmed. Microscopically, the study groups with Mentha piperita L. showed mild to moderate damage with significant differences from the untreated control group. The evaluation of hepatoprotective potential on the M. piperita L. extract at doses studied did not behave as hepatoprotective agent...

Se realizó un estudio farmacológico preclínico para evaluar el efecto hepatoprotector de Mentha piperita L. frente a la toxicidad inducida por el paracetamol. Se emplearon ratones adultos machos NMRI a los que se administró por vía oral extractos blandos de la planta a dosis de 200 mg/kg y 400 mg/kg, tres días consecutivos previos a la inducción de la hepatotoxicidad. Se evaluaron los signos clínicos de toxicidad, parámetros bioquímicos hepáticos y el análisis morfológico del hígado. Los parámetros bioquímicos analizados mostraron diferencias altamente significativas, pero ninguno de los dos grupos presentaron un comportamiento similar al grupo control no tratado. No se confirmaron alteraciones macroscópicas del hígado. A nivel Microscópico, los grupos en estudio con Mentha piperita L. presentaron daños de leves a moderados con diferencias significativas respecto al grupo control no tratado. Se puede afirmar que según la evaluación del potencial hepatoprotector del extracto de M. piperita L. a las dosis estudiadas no se comportó como agente hepatoprotector...

Chemical components of essential oil of Mentha piperita L. collected from Sapa – Lao Cai and now cultivated in Co Nhue, Tu Liem district – Ha Noi

Mentha piperita L.Mentha piperita L. was collected from Sapa, Lao Cai province and cultivated at Co Nhue – Tu Liem – Ha Noi. The content of the essential oil in fresh branches of this peppermint was about 0.16%. Its green yield is 1.85-2.10 Kg/m2. First time in Vietnam, the chemical composition of peppermint oil was analyzed by GC/MS and 43 constituents were identified. The main constituents were L-menthol (47.60%), menthol (24.10%), menthofuran (6.07%) and 1.8 cineol (5.55%), pulegone (4.22%). Base on these data. The quality of Sa Pa peppermint oil is good as one from native origin. Peppermint collected from Sa Pa enrich the gene source of medicinal plant in general and particularly the gene source of essential oil plant group of Vietnam