Chemical constituents from stems of Uncaria scandens / 中草药

Objective: To study the chemical constituents from the stems of Uncaria scandens. Methods: The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, RP18, and HPLC. The structures were identified by spectroscopic methods and physicochemical properties. The contents of rhynchophylline and isorhynchophylline were detected by HPLC method. Results: Seventeen compounds were isolated and identified from 95% ethanol extract of the stems of U. scandens and characterized as 5α-carboxystrictosidine (1), 3β-isodihydrocadambine (2), strictosamide (3), 3α-dihydrocadambine (4), mitraphylline (5), uncarine F (6), 3β-isodihydrocadambine 4-oxide (7), isodihydrocdambine (8), nauclefine (9), cadambine (10), calyxamine B (11), methyl chlorogenate (12), 3,4,5-trimethoxyphenol (13), α-tocopherylquinone (14), quinovic acid (15), methyl caffeate (16), and hyperoside (17). The contents of rhynchophylline and isorhynchophylline from U. scandens were 0.72 and 0.38 μg/g. Conclusion: Compounds 7-9 and 11-14 are isolated from Uncaria genus for the first time. Compounds 1-6 and 16, 17 are isolated from U.scandens for the first time. The contents of rhynchophylline and isorhynchophylline is low and the reasonable use of them as U. scandens in some areas needs to be further verified.

Phenolic acid compounds from anemone altaicae rhizoma / 中国药学杂志

OBJECTIVE: To study the chemical constituents in the roots of Anemone altaica. METHODS: The isolation and purification of the compounds were performed by chromatography on Diaion HP-20, Sephadex LH-20, and silica gel, combined with preparation liquid chromatography. Their structures were determined by comparison of their physicochemical characteristics and spectral data with literatures. RESULTS: Eleven compounds were obtained, and their structures were identified as methyl feruloyl-tartarate A (I), methyl feruloyl-tartarate B (II), ethyl feruloyl-tartarate B (III), mono-methyl feruloyl-lactate (IV), dimethyl feruloyl-lactate (V), vanillic acid-4-O-β-D-glucopyranoside (VI), p-hydroxybenzoic acid glucoside (VII), methyl chlorogenate (VIII), 3-O-fernloylquini acid (IX), 5-O-feruloyl-3-O-(β-D-glucopyranosyl)-2-deoxy-D-ribono-γ-lactone (X), and carboxymethyl isoferulate (XI). CONCLUSION: Compounds I-V are new compounds, and are speculated to be a man-made products. Compounds VI-XI are isolated from the plants of anemone genus for the first time.

Chemical constituents from seeds of Ferula sinkiangensis / 中草药

Objective: To study the chemical constitutions from the seeds of Ferula sinkiangensis. Methods: Using different chromatographic methods to isolate and purify the compounds and to identify their structures by physicochemical properties and spectroscopic technology. Results: Thirty-two compounds were isolated and elucidated as butanedioic acid (1), variecolorquinonesa (2), β-sitosterol (3), stigmasterol-3-O-glucoside (4), 1-(3-ethylphenyl)-1,2-ethanediol (5), pinoresinol (6), (7,8-cis-8,8'-trans)- 2',4'-dihydroxyl-3,5-dimethoxy-lariciresinol (7), secoisolariciresinol (8), n-hexacosanol (9), isoquercitrin (10), quercetin (11), ferulic acid (12), stigmasterol (13), luteolin-7-O-β-D-gluronide (14), (R)-2'-hydroxy-N-[(2S,3S,4R)-1,3,4-trihydroxyhexacosan-2-yl] nonadanamide (15), macrathoinf (16), 1,5-di-O-caffeoylquinic (17), arctiin (18), parvifoliolsg (19), 5-hydroxymethyl fufural (20), daucosterol (21), vanillic acid (22), sucrose (23), rutin (24), neoarctina (25), methyl chlorogenate (26), 5-O-caffeoylquinic (27), uridine (28), inosine (29), 7-hydroxy coumarin (30), chlorogenic acid (31), and caffeic acid (32). Conclusion: Compounds 2, 5-11, 14-20, 22, 24-29, 31, and 32 are isolated from the plants of Ferula L. for the first time.

Nonflavonoid constituents from leaves of Apocynum venetum / 中草药

Objective: To study the nonflavonoid constituents in the leaves of Apocynum venetum, the medicinal halophyte. Methods: The constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography as well as preparative HPLC, and their structures were elucidated by means of physicochemical properties and spectrosocpic analyses. Results: Fifteen nonflavonoid compounds were isolated from the methanol extract from the leaves of A. venetum, and identified as scopoletin (1), esculetin (2), methyl chlorogenate (3), chlorogenic acid (4), grasshopper ketone (5), benzyl-O-β-D-glucopyranoside (6), 2-phenylethyl-O-β-D-glucopyranoside (7), 1-β-O-benzoyl-D-glucopyranoside (8), tyrosol (9), isovanillic acid (10), vanillic acid (11), protocatechuic acid (12), lupeol (13), β-amyrin (14), and α-linolenic acid (15). Conclusion: Compounds 2, 3, 5-10, 14, and 15 are isolated from this plant and the plants in Apocynum Linn. for the first time.