HPLC analysis of 5-methylcytosine contents in DNAs isolated from 5-azacytidine and MNNG treated cells / 中国病理生理杂志

The 5-methylcytosine (~mC) in DNAs from 5-azacytidine and MNNG treated FL, Wish and Veto-E6 ceUs were analysed by HPLC. In 2?10~(-6)mol/L 5-azaCR treated cells, the percentages of ~mC in total cytosine were all lowered significantly (P 0.05). These results were in good agreement with those obtained by radioactivity analysis of newly replicated DNA fragments from Hpa Ⅱ digest. These results further validate the idea that DNA hypomethylation as a general pathway in the initiation process of chemical carcinogenesis is based on the results obtained by a defectively designed experiment.