RESUMO
OBJECTIVE: A micellar electrokinetic capillary chromatography(MECC) method was established for determination of benzoic acid and salicylic acid in Zuguang san.METHODS: An uncoated silica capillary(75 ?m?55 cm,effective length 47 cm) was used with 20 mmol?L-1 borate-30 mmol?L-1 SDS-4% methanol(pH 9.8) as the running buffer solution.The internal standard was antiscorbic acid.The detection wavelength was set at 250 nm.RESULTS:The calibration curves were linear in the range of 120～320 ?g?mL-1 for benzoic acid(r=0.999 8) and 80～320 ?g?mL-1(r=0.999 5) for salicylic acid.The average recoveries were 98.37% and 100.69%,respectively and the RSD were 4.47%(n=9) and 1.61%(n=9),respectively.CONCLUSION: MECC method was proved to be simple,sensitive and reproducible with high recovery rate and it is applicable for the determination of the contents of benzoic acid and salicylic acid in Zuguang san.
RESUMO
Object To isolate and identify the four active constituents, chrysophanol, emodin, rhaponticin and rhein in rhubarb Methods Micellar electrokinetic capillary chromatography (MECC) with a buffer solution of 5 mmol/L H 3BO 3 NaOH containing 20 mmol/L of sodium deoxycholate (SDC) (pH=10) was used for their separation at a voltage of 17 kv Results The four active constituents were completely separated within 5 min Conclusion This method was found to be simple and rapid and gave satisfactory results