Effect of MicroRNA-9 on cell proliferation and collagen synthesis in hepatic stellate cells by down-regulation of TGFBR2 / 中国医师杂志

Objective To investigate the effect of MicroRNA-9 (miR-9) on cell proliferation and collagen synthesis in hepatic stellate cells (HSCs),and to explore the potential mechanism.Methods HSC-T6 cells were cultured and transfected with miR-9 mimics with lipofectamine 2000.After incubation 48 h,the cells were collected and total proteins and RNAs were extracted.The expression of miR-9 was detected by quantitative real time polymerase chain reaction (qRT-PCR).The protein expression of type Ⅰ collagen and type Ⅲ collagen were measured by Western blot.The methyl thiazolyl tetrazolium (MTT) method was used to asses the proliferation of HSC-T6 cells.The expression of transforming growth factor-β1 receptor 2 (TGFBR2) was detected by qRT-PCR and Western blot.Results Compared to the control group,miR-9 expression in HSCs was increased in the miR-9 mimics group (P ＜ 0.05),type Ⅰ and type Ⅲ collagen protein expression was reduced by (44 ± 2) ％ and (50 ± 3) ％ (P ＜ 0.01),respectively.The proliferation activity of HSCs was decreased by (48 ± 4)％ (P ＜ 0.05).The expression of TGFBR2 was inhibited in the miR-9 mimics group.Conclusions Upregulation of miR-9 plays a role on suppressing cell proliferation and collagen synthesis in HSCs.This process might be mediated by downregulation of TGFBR2.

Let-7a is involved in the pathogenesis of chronic eosinophilic rhinosinusitis with nasal polyp by regulating BAFF / 中国医师杂志

Objective To explore the regulatory effect of miRNA on B-cell activating factor (BAFF) in chronic eosinophilic rhinosinusitis with nasal polyp (Eso CRSwNP).Methods Ten nasal mucosal samples were collected from Eso CRSwNP patients who were admitted and treated in our hospital between January 2012 and February 2013.Normal nasal mucosal tissues (n =10) served as control.The miRNA-targeting BAFF was predicted by bioinformatics tools.Immunohistochemistry and real time polymerase chain reaction (RT-PCR) were used to assess the protein expression of BAFF and the predicted miR-NA.The correlation between the predicted miRNA and BAFF was analyzed.Results Down-regulated expression of let-a was confirmed in Eos CRSwNP,while the BAFF protein expression was increased.Let-a was positively correlated with BAFF in nasal epithelia.Conclusions Let-a might contribute to mucosal eosinophilia in eosinophilic CRSwNP via targeting BAFF.

Effect of miR-218 on proliferation and cell cycle of gastric cancer cells / 中国医师杂志

Objective To investigate the effect of miR-218 on cell proliferation and cell cycle in the gastric cancers (GC).Methods SGC-7901 and MGC-803 cells were transfected with miR-218 mimics.Meanwhile,SGC-7901 and MGC-803 cells were transfected with control mimics (Scramble mimics) as negative control.Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the miR-218 expression in these transfected cells.The effect of miR-218 overexpression on cell proliferation was evaluated with direct cell counting and MTS [3-(4,5-dimethylthiazol-2-yl)-5 (3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium inner salt] assay.Moreover,the effect of miR-218 overexpression on cell cycle was examined by fluorescence activated cell sorting (FACS).Results miR-218 expression was remarkably induced in miR-218-transfected cells,miR-218 overexpression led to a significant decrease in cell proliferation rate compared to control cells (P ＜ 0.05).miR-218 overexpression induced GC G1 arrest.Conclusions miR-218 can suppress GC cell proliferation and block cell cycle progression.It maybe play an important role in tumorigenesis and development of GC.