Comparison of E. coli Infiltration between New Synthetic Absorbable Sutures

PURPOSE: The proper selection of suture is very important to minimize infection after gastrointestinal anastomosis and closure, which is one of the causes of postoperative complications such as leakage and stricture, etc, in the surgical field. Thus this study focuses on which suture can reduce bacterial infection after surgical operation by comparing in vitro microbial infiltration rates of three synthetic absorbable sutures and that of silk - a relatively absorbable material, using E. coli. METHODS: Four different, sterilized kinds of absorbable sutures were used for two experiments. In experiment 1, the cut-off suture was directly applied to the standard method agar plate and cultured for observation. In experiment 2, the cut-off suture was diluted with 1 ml of tryptic soy broth to be smeared and cultured in the standard method agar plate and counted using a spectrophotometer. RESULTS: The first experiment revealed that bacterial growth was not observed in the monofilament and antibiotic-coated multifilament sutures, while the other sutures of multifilament structure were invaded by bacteria. In the second experiment, counting and averaging the colony from five plates of each test showed that the number of E. coli of monofilament suture, antibiotics-coated polyglactin, polyglactin and silk were 0+/-0, 39.3+/-14.4, 208.6+/-76.6, 59.4+/-26.7, respectively. CONCLUSION: Sutures of monofilament structure are believed to be a relatively safe material that can be used for gastrointestinal anastomosis and closure since it has lower bacterial infiltration rates than sutures of multifilament structure.

Infiltração microbiana em dentes portadores de restaurações provisórias / Microbial leakage in teeth with temporary restorative materials

O objetivo deste estudo foi avaliar a infiltração microbiana em dentes restaurados provisoriamente com omaterial restaurador intermediário (IRM, Intermediate Restorative Material), Cavit ou Vitremer. Para tanto,foram utilizados 50 dentes humanos extraídos unirradiculares, distribuídos aleatoriamente em três gruposexperimentais e dois grupos controle (positivo e negativo). Para o modelo de estudo, empregou-se uma plataforma,dividida em duas partes: câmara superior - onde foi introduzida a suspensão microbiana contendoos indicadores biológicos (E. faecalis + S. aureus + P. aeruginosa + B. subtilis + C. Albicans); e, câmarainferior, com o meio de cultura (Brain Heart Infusion), onde os dentes permaneceram imersos com 5 mm doremanescente apical radicular (correspondente ao terço cervical) durante o período de 60 dias. Observou-sea partir dos resultados, que nos dentes dos grupos 1 (IRM) e 2 (Cavit) ocorreu infiltração microbiana a partirde 7 dias. No grupo 3 (Vitremer) não foi verificada infiltração microbiana no período de 7 a 60 dias.

This study aimed to determine the microbial coronal leakage of temporary restorative materials when employingIRM, Cavit or Vitremer, by means of differents microbial indicators. Thus, 50 single-rooted humanteeth were used, which were shaped until the file size 50 and assigned to 3 experimental groups. Two groupswas used as control. In the study model, a platform was employed, which was split in two halves: an upperchamber - where the microbial suspension containing the biological indicators was introduced (E. faecalis +S. aureus + P. aeruginosa + B. subtilis + C. albicans); and a lower chamber containing the culture mediumBrain Heart Infusion, in which 5 mm of the apical region of teeth (coronal third) were kept immersed. Interpretationsof the time to occur microbial leakage were made daily for 60 days, using the turbidity of the culturemedium which is indicative of microbial contamination, as a reference. The results showed that in the teethfrom groups 1 (IRM) and 2 (Cavit) occurred microbial leakage after 7 days. In the group 3 (Vitremer) it wasnot observed microbial leakage at intervals of 7 to 60 days.