RESUMO
Background: Preterm labour occurs due to various causative factors. Genital infections contribute significantly to the preterm labour. Ours is a prospective, observational study looking at the cervical microbiota involved in the preterm labour. The cervical flora contributing to the occurrence of preterm labour was studied. The results were analyzed based on the microbiological study of cervical swabs. The primary objectives of this study were to determine the association of cervical microbiota of pregnant women with spontaneous preterm delivery. To characterize the effect of cervical microbiota on birth gestation. The secondary objective of this study was to analyse the role of cervical microbiota and its relationship between social and behavioural factors.Methods: It is a prospective, observational study, which includes 193 patients, studied over a period of one and a half year. It was conducted at our tertiary care centre after the approval by ethics committee, and the results were analysed. As this is a purely observational study, management decisions of treating obstetrician were not interfered with. No additional cost, intervention or injury was caused to the study subject.Results: In our study, cervical swab culture shows growth in 2.07% which is statistically insignificant. Though, it was found that preterm labour is more common with leucocytosis, i.e. WBC > 11000 cmm, which is found to be statistically significant (p value = 0.023).Conclusions: In our study, we found that there was no significant association of cervical flora with preterm labour, though we found an association with leucocytosis.
RESUMO
Background: Infection is a health hazard of great expense and significance affecting the final outcome of treatment. Hospital indoor air contains a diverse range of microbial population. Objective: To study microbiological organism and their antibiotic sensitivity isolated from patients’ blood culture and to correlate this micro-organism with pattern of microbiological flora in tertiary care hospital. Materials and methods: All the patients who fulfilled study criteria were subjected for blood culture analysis and prospectively observed for clinical conditions and there outcome. Antimicrobial susceptibility testing was performed for all blood culture isolates. Case fatality study was a comparison between deaths in blood culture positive patients with reference to resistance to first line antibiotics. A comparison was made between the type of organism found in cultures of samples taken from patients and the type of organism obtained from sites near the patients during the same time interval. Results: out of 3144 sample sent for blood culture, 888 (28.2%) samples came out to be positive. most isolates of klebseilla, Acinobacter, and E. coli were resistant to ampicillin (97 to 98%), gentamycin (70 to 80%) and cephalosporins (65% to 100%). Most of the gram negative isolates were sensitive to levofloxacin and Piperacillin-tazobactam. Staphylococcus aureus were resistant to Parikh YN, Kharadi J, Nain N, Kalathiya M, Shah Z. Bacterial isolates from the Pediatric patients and its correlation with microbiological flora in tertiary care hospital. IAIM, 2017; 4(1): 78-84. Page 79 ampicillin (90.4%). Staphylococcus aureus were sensitive with vancomycin by 98.6%. Case fatality due to gram –ve organisms were 19.45%. Gram –ve bacteria were dominating pathogens in both prefumigation samples from various sites of ICU and wards and blood culture sample from patients admitted in ICU and wards i.e. 32 (72.7%) and 815 (91.8%) respectively. It sets up a correlation between source of infection and sepsis in patients admitted to ICU and wards. Conclusion: Most organism (gram positive and gram negative) were resistant to first line antibiotics such as ampicillin, gentamycin, cephalosporins. Antibiotic combination with piperacillintazobactam or levofloxacin had better sensitivity among all gram negative organisms. Gram positive organisms were susceptible to vancomycin. Most deaths occur in patients infected with first line antibiotic resistant strains. Strong association found between source of bacterial infection and culture sample positivity.