RESUMO
Objective: The co-processd excipient containing microcrystalline cellulose (MCC) and glycerol monostearate (GMS) was prepared. The humidity resistance of MCC used in Chinese materia medica (CMM) was improved and the possibility of this co-processd excipient used in the preparation of CMM tablet was explored. Methods: The co-processd excipient containing MCC and GMS was prepared by applying spray drying method. The moisture absorption, flow ability, compressibility, and disintegration were as the indexes to optimize the best preparation of co-processd excipient through uniform design methodology. The powder characteristic of co-processd excipient was investigated and the microstructures were studied by scanning electron microscope (SEM), differential scanning calorimeter (DSC), X-ray diffraction (XRD), and Flourier transform infra-red (FTIR) spectroscopy. The effect of co-processd excipient on the moisture absorption of spray drying power of Xinyueshu, epimedium total flavonoids, and wolfberry extracts was investigated. The in vitro dissolution of hyperside and ferulic acid in Xinyueshu Tablet was studied. Results: The results showed that the optimal conditions of the preparation consisted of 1 portion of GMS, 12 portions of MCC, and 200 portions of water, emulsionzing temperature of 66°C, and mixing time of 1 h. It was confirmed that the chemical constituents in co-processd excipient were not changed after spray drying with smaller particle size and better fluidity. The co-processd excipient can improve the moisture absorption of spray drying power of Xinyueshu, epimedium total flavonoids, and wolfberry extracts without affecting the disintegration of MCC. Conclusion: The co-processd excipient prepared has good humidity resistance and it is confirmed to have application prospect.
RESUMO
Having collected the skull of a fallen adult victim,and made artificial fractures on an autopsled adult skull as well as on two living dog skulls,we observed the edges of skull fractures under the scanning electron microscope in order to find out the submicroscopic morphologic features of skull fractures aiming at elucidating the mechanism of fracture,and differentiating ante-from post-mortem fractures.Many microfractures were found along the main fracture lines,they extended into the interior of the skull. The width of the microfractures varied from 5 to 100?m.Some of them were located in the external compact bone and some between the outer compact bone and diploe. The rest were found in the diploe in the form of transverse,longitudinal or spiral fractures of bony trabeculae.Collagenous fibre bunches embeded in the bony matrix were broken and separated. The blood vessels inside the skull were crosscut or longitudinary torn by the fracture lines. Sometimes the torn blood vessels were pulled out of the Harversian canals.In antemortem fractures as a results of hemorrhage, there were not only blood clot on the edges of the broken skull, but also hemorrhage into the microfractures. The fibrin networks and red blood cells were easily found in the depths of these cracks.A deductive mechanism of formation of the microfractures and the possibility of differentiation between ante-and post-mortem fractures are discussed.