Study on the Lipid-lowering Effect and Potential Mechanism of the Couplet Medicines of Flastem Milkvetch Seed and Tribulus Terrestris on Hyperlipidemia Rats / 世界科学技术-中医药现代化

Objective: To explore the lipid-lowering effect and potential mechanism of the couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris on hyperlipidemia rats. Methods: Forty-eight Sprague-Dawley rats were randomly divided into 6 groups, normal control group, high-fat model group, simvastatin group, Tribulus terrestris group, Flastem Milkvetch Seed group, and couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris group, with 8 rats in each group. The normal control group was fed with basal diet, and the other groups were fed with high-fat diet to establish a hyperlipidemia rat model. At the same time, different group rats were treated with different drug respectively. 6 weeks later, the body weight and visceral index of the rats were measured. Levels of serum cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) were detected by automatic biochemical analyzer. The mRNA and protein expression of HMG coenzyme A reductase (HMGCR), cholesterol 7-alpha hydroxy-lase (CYP7A1) and low density lipoprotein receptor (LDL-R) were detected by RT-PCR and western blot. Results: Compared with model group, the serum levels of TC, TG, and LDL-C were significantly decreased in all Chinese medicine groups, and the serum level of HDL-C was significantly increased in Flastem Milkvetch Seed group and couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris group. Moreover, the mRNA and protein expression of HMGCR, CYP7A1, and LDL-R in rat liver also significantly increased in all Chinese medicine treatment groups. And couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris group shows more significantly effect. Conclusion: Couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris significantly regulate the serum lipid levels on hyperlipidemia rat model, and its mechanism may be related to the regulating expressions of HMGCR, CYP7A1, LDL-R gene.

Effects of Compound Preparation of Milkvetch Root and Breviscapine on Memory Ability and SOD, MDA and LDH in Blood and Brain of Alzheimer's Disease Rats / 中国药师

Objective: To observe the effect of compound preparation of milkvetch root and breviscapine (HDs) on the activity of superoxide dismutase (SOD) and lactate dehydrogenase(LDH), the concentration of malondialdehyde (MDA) in blood and brain and memory impairment in Alzheimer's disease rats.Methods: The rats were randomly divided into 5 groups (10 rats /group): the normal control group, Alzheimer's disease (AD) model group induced by AlCl3(5 mg·kg -1·d-1,ig) and D-gal (40 mg·kg-1·d-1,ip), and 3 AD groups respectively treated with different drugs including piracetam (0.15 g·kg-1·d-1,ig), HDs1(1.5 ml·kg-1·d-1,ip) and HDs2 (3 ml·kg-1·d-1,ip).After 90-day treatment, the step-down test was used to detect the learning and memory ability, and SOD and LDH activity and MDA concentration in blood and brain were examined as well.Results: Compared with that in AD model group, the ability of learning and memory was improved, the activity of SOD and LDH increased significantly, the concentration of MDA decreased significantly in blood and brain in HDs treated groups.The differences were statistically significant (P<0.05 or P<0.01), but they were not restored to normal levels.Some indexes of HDs2 group were better than those of piracetam positive control group and HDs1 group (P<0.05 or P<0.01).Conclusion: HDs can effectively reduce MDA concentration, increase SOD and LDH activity in blood and brain, and improve the ability of learning and memory of AD rats.

Clinical Study on Treatment of Infantile Post-infection Cough with Milkvetch Root and Radix Glycyrrhizae / 国际中医中药杂志

Objective To analyze the relationship between clinical features of infantile cough after infection and microelements in peripheral blood, and to observe the therapeutic of treating this cough with Milkvetch Root and Radix Glycyrrhizae. Methods 75 infantile patients were randomly recruited into a therapeutic group and a reference group. 39 patients in the therapeutic group were treated with Huangqi Zhusheye and Tablete Glycyrrhiza, while 36 patients in the reference group were treated with Azithromycin. The therapeutic effects were evaluated after the treatment. Meanwhile,microelements in peripheral blood of the 75 patients were assayed and made a contrast to 40 healthy children. Results The contents of Fe, Zn, and Mg in peripheral blood of the 75 patients dropped on different levels, comparing to the healthy children.The therapeutic effects of the therapeutic group was much better than the reference group with significant difference (P＜0.05).Liver and renal functional lesion was unseen in both groups after the treatment. Conclusion Infantile past-infection cough may related with the decrease of Mg, Fe, and Zn. Combined usage of Milkvetch Root and Radix Glycyrrhizae clinically has good therapeutic effects to this disease.

Study on Moulding Technics of Anxinkang Drop Pills / 浙江中医药大学学报

[Objective]To study the optimum techniques making Anxinkang drop pills. [ Methods ] Using parallel experimentation for the choice of Matrix and refrigerant,dropping distance and rate,the temperranture of refrigerant.Using orthogonal experimentation for the choice of proportion of extract and Matrix,proportion of extract,water and glycerin, the temperature of drug solution.with quality of outward appearance as the evalution quota to decide the best dropping condition.[ Results ]The optimum technique was that PEG6000∶PEG4000(3∶2) as matrix,Methyl-siliconoil:liquid-paraffin(3∶1) is refigerant.Internal and external diameter of burette are equal to 2.5mm and 3mm .Drug is dropping into the refrigerant of about15~20 drop per minute [ conclusion ] The oplimum techniques are feasible and accord with the quality standards of drop pills.

The Study on the Effects of the Components of Mongolian Milkvetch Root and Red Sage Root Compound on the Expression of Matricial Metal Protease-9 (MMP-9) mRNA on Placental Nourish Cells and the Blood Plasma Level of IL-10 in Model Pregnant Rats / 中国中医药信息杂志

Objective To explore the molecular mechanism of elevating placental blood supply by the component from Mongolian Milkurtch and red sage Root. Method Abstract the effective components from Mongolian Milkurtch and red sage Root, apply L-Arginine methy1 ester (L-NAME) to make NO production blocking model, use ELISA to check IL-10 in plasma of 18 d pragant rats, and FISH to check the expression of matricial metal protease-9 (MMP-9) mRNA. Results The plasma level of IL-10 of model group was lower than that of blank group (P 0.05). Conclusion By interving the relationship between IL-10 and MMP-9, the components of Mongolian milkvetch root and red sage root compound may affect the shift and invadation of deciduas, so as to prevent fleet embedding, help building the circulation between mother and embryo, and keep the placental blood supply.

Effect of menbranous milkvetch root parenteral solution on insulin resistance of gerontism cerebral infarction in stage of recovery / 中国康复理论与实践

@#ObjectiveTo study the effect of menbranous milkvetch root parenteral solution on insulin resistance of gerontism cerebral infarction in recovery stage.Methods66 patients with gerontism cerebral infarction were randomly divided into therapy group(33 cases) which received membranous milkvetch root parenteral solution and control group(33 cases).Both groups adopted routine treatment at the same time, the period was 20 days. Insulin(Ins), free blood sugar(FBS), total cholesterol(CH), triglyceride(TG), hemorheology and insulin resistance(indicating by index of insulin sensitive) of blood on empty stomach were evalutated before and after treatment. ResultsAfter treatment there was decrease in CH, FBS, FINS in therapy group than in control group(P<0.01 or P<0.05); the clinical effect in therapy group was better than in control group(P<0.01).ConclusionMembranous milkvetch root parenteral solution can significantly decrease insulin resistance, blood lipin, blood viscosity in recovery stage of gerontism cerebral infarction, and improve clinical efficiency.

Effects of treatment with milkvetch root and red sage root injections on changes in blood viscosity and renal function in patients with early diabetic nephropathy / 中国实用内科杂志

Objective To investigate the influence of treatment with milkvetch root and red sage root injections on changes in blood viscosity and renal function in patients with early diabetic nephropathy(EDN).Methods 160 patients with EDN were randomly divided into two groups.The control group (n=80) was treated with conventional therapy for diabetes,and the treatment group (n=80) was treated with milkvetch root injection combined with red sage root injection based on conventional therapy for 4 weeks.Results After the above-mentioned therapy the parameters of blood viscosity were significantly decreased(P

Neuroprotection effect of Milkvetch injection in neonates with hypoxic-ischemic-encephalopathy / 中国临床药理学与治疗学

(0.05)).There had significant difference((25.47)?(8.27) vs(32.40)?(5.86)) between them at the seventh day(P