Modified Dihuang Decoction improves ovarian reserve in mice by regulating Bcl-2-related mitochondrial apoptosis pathway / 中国中药杂志

The present study investigated the effect of Modified Dihuang Decoction in improving ovarian reserve in mice through the Bcl-2-related mitochondrial apoptosis pathway. Forty-eight adult female BALB/c mice were randomly divided into the following six groups with eight mice in each group: a blank group, a model group, a femoston group(three cycles of treatment with 0.13 mg·kg~(-1) estradiol tablets for 2 days and 1.43 mg·kg~(-1) estradiol and dydrogesterone tablets for 3 days), and high(64.74 g·kg~(-1))-, medium(43.16 g·kg~(-1))-, and low-dose(21.58 g·kg~(-1)) Modified Dihuang Decoction groups. Mice in other groups except the blank group received a single intraperitoneal injection of 12 mg·kg~(-1) cyclophosphamide and 1.2 mg·kg~(-1) busulfan to induce a model of diminished ovarian reserve(DOR), while those in the blank group received an equal volume of normal saline. Mice were treated with corresponding drugs for 15 d from the 36 th day, once per day, and the mice in the blank group and the model group were treated with an equal volume of normal saline. The general condition and oestrous cycle were observed. The serum hormone levels were detected with the enzyme-linked immunosorbent assay(ELISA). The morphological changes of ovaries were observed by HE staining. Western blot was used to detect the protein expression of cysteinyl aspartate specific proteinase-9(caspase-9), cleaved caspase-3, Bcl-2 associated X protein(Bax), Bcl-2, superoxide dismutase-2(SOD-2), and glutathione peroxidase-1(GPx-1). The mRNA expression of Bax and Bcl-2 was detected by real-time fluorescence-based quantitative polymerase chain reaction(real-time PCR). The results showed that compared with the blank group, the model group showed body weight loss, disordered oestrous cycle, elevated serum levels of follicle-stimulating hormone(FSH) and luteinizing hormone(LH), reduced serum levels of estradiol(E_2), anti-mullerian hormone(AMH), and inhibin B(INHB), the declining number of ovarian follicles and granulosa layers, increased number of atretic follicles, up-regulated protein expression of caspase-9, cleaved caspase-3, and Bax and Bax mRNA expression in ovaries, and down-regulated protein expression of Bcl-2, SOD-2 and GPx-1, and Bcl-2 mRNA expression. Compared with the model group, the Modified Dihuang Decoction groups displayed restored body weight and oestrous cycle, decreased serum levels of FSH and LH, elevated serum levels of E_2, AMH, and INHB, increased number of ovarian follicles, thickened granulosa layers, and declining number of atretic follicles. Additionally, the protein expression of caspase-9, cleaved caspase-3, and Bax, and Bax mRNA expression was down-regulated, and the protein expression of Bcl-2, SOD-2, and GPx-1, and Bcl-2 mRNA expression was up-regulated. The results suggest that Modified Dihuang Decoction can regulate endocrine hormone, promote follicle growth and improve ovarian reserve by enhancing ovarian anti-oxidant capacity, inhibiting the Bcl-2-related mitochondrial apoptosis pathway, and further inhibiting cell apoptosis.

Study on the Prevention of Relapse of Urinary Calculus with Modified Dihuang Decoction / 国际中医中药杂志

Objective To studythe preventive effects of modified Dihuang decoction on the relapse of urinary calculus after caiculus removed or destroyed.Methods 306 cases were randomly divided into a trealment group (with 149 cases)and a control group(with 157 cases).The trealment group was interfered with modified Dihuang decoction for 6 years,and the control group was not interfered with any medicine.Observe the relapse number in the two groups.Results There were 8 cases relapsed in the treatment group(5.4%)comparing with 46 cases relapsed in the control group(29.3%),showing a significant difference (P＜0.01).Conclusion Medified Dihuang decoction has apparent effects in preventing relapse of urinary calculus.

Clinical Efficacy of Modified Dihuang Decoction on the Treatment of Primary Nephritic Syndrome and Its Effect on Cytokines / 中国中医药信息杂志

Objective To observe the clinical efficacy of modified Dihuang decoction combined with conventional western medicine for the treatment of primary nephritic syndrome. Methods One hundred and eight cases of primary nephrotic syndrome were randomly divided into treatment group (54 cases) and control group (54 cases). Treatment group was given modified Dihuang decoction combined with conventional western medicine, the control group was treated with conventional western medicine only. IL-6, IL-8 and TNF-? content in blood of the two groups were observed before treatment and one month after treatment. Results The therapeutic effect of the treatment group was better than the control group (P

Effect of modified Dihuang decoction on expression of c-fos positive neuronin in hippocampal CA1 area in diabetic rats / 中华中医药杂志

Objective:To investigate the effect of modified Dihuang decoction on expression of c-fos positive neuron in hippocampal CA1 area in diabetic rats. Methods:The diabetic rat models were induced by intraperitoneal injection of streptozotocin(STZ) . 4 weeks later,the expression of c-fos was detected by immuno-histochemistry. Results:Compared with diabetes group(9.25?2.36,0.56 ?0.06) ,the positive cell's density and average light density(8.13?1.70,0.46?0.08) of c-fos positive neuron in hippocampal CA1 area in diabetic rats which were prevented and treated with modified Dihuang decoction both decreased(P