RESUMO
Objective: The genetic diversity of wild Bletilla striata from different sources was analyzed in order to provide the reference value for breeding, protection and development of B. striata germplasm in the future. Methods: Genetic diversity of 50 wild B. striata samples was identified by SRAP molecular marker technique, and the genetic relationship was analyzed by UPGMA method. Results: A total of 117 bands were amplified from 50 samples by using 15 SRAP primer combinations, with an average of 7.8 bands per primer. Among them, 106 bands were polymorphic, with a polymorphism rate of 90.60%. The clustering results showed that the genetic similarity coefficient of 50 B. striata samples was 0.59-0.87, which could be divided into six categories based on the genetic coefficient of 0.66. The genetic distance of two pieces of B.striata from No.2 Lijiang, Yunnan and No.2 Jingshan County, Hubei was not directly related to geographical distance. Conclusion: There are abundant genetic diversity among wild B. striata resources, and there is no direct relationship between geographical distance and genetic distance. SRAP technology can provide theoretical basis for the protection of B. striata resources and variety breeding, which provides reference for the subsequent development and utilization of B. striata.
RESUMO
Different subspecies or strains of the same species produce varied clinical manifestations.The clarifica-tion of parasite taxonomy is useful for the researches of their biology, epidemiology and control.DNA molecular markers have the advantages of high polymorphism, non-pleiotropy, and clear identifying alleles, etc.This paper summarizes the first generation(restriction fragment length polymorphism, random amplified polymorphic DNA), second generation(sim-ple sequence repeat-anchored PCR, inter-simple sequence repeat) and third generation(single nucleotide polymorphism) molecular marker techniques, and their application in taxonomic identification of parasites.