Immunoaffinity Chromatography Method for Purification of Human Protein C / 中国输血杂志

A special monoclonal antibody(McAb)to protein C(PC)was prepared,itrevealed the different conformation in the absence and presence of Ca~(++).This McAbwas coupled to Sepharose-4B and the gel was loaded into the column for thepurification of PC.The immobilized antibody was capable of binding PC fromthe plasma in the absence of Ca~(++).This immunopurification resulted in a 12 000-fold after one step proceduce.The specific activity of the PC product ranged be-tween 180-230 U/mg and behaved as two bands(?-PC and?-PC)on nonreducedSDS-PAGE and five bands(?-S-PC,?-S-PC,?-heavy chain,?-heavy chainand light chain)on reduced SDS-PAGE.