Effect of Angelica sinensis radix on colonic morphology and mucus secretion of experimental XuexuBianmi model mice / 中国应用生理学杂志

OBJECTIVE@#To study the effects of Angelicaesinensis radix (Danggui) decoction on the therapeutic action and the colonic morphology and mucus secretion in XuexuBianmi model mice.@*METHODS@#Sixty Kunming mice, maleandfemaleinhalf, were randomly divided into six groups according to gender and weight (=10):normal control group, XuexuBianmi model group, positive control group, and high-dose, middle-dose and low-dose Danggui groups. Except the normal control group, the mice in the rest groups were orally administrated with diphenoxylate (DPN) and subcutaneously injected with acetylphenyhydrazine (APH) and intraperitoneally injected with cyclophosphamide (CPA) to copy XuexuBianmi model. Fromthe 14th day, the mice in Danggui groups were orally administered with different doses of Danggui decoction (16.67、8.33、4.17 g/kg), the mice in positive control group were orally administered with Changtongshu granule (5 g/kg), the mice in XuexuBianmi model group were administered with normal saline (NS) at the same volume (10 ml/kg), once a day for consecutive 28 days. The general status were observed, the first black defecation time (FBDT), the water content in stool and in colon in mice of all groups were tested. And the colon tissue was stained with hematoxylin-eosin (HE) and AB-PAS to observe the changes of colonic morphology and the mucus secretion.@*RESULTS@#Compared with the normal control group, there appeared the XuexuBianmi syndromes in model group as follows, the defecation time (FBDT) was significantly prolonged, the water contentinstool and in colon were decreased (<0.01), the colonic mucosa and gland were atrophied, mucous membrane layer was thinned (<0.01), mucus secretion was decreased. Compared with the XuexuBianmimodel group, the Xuexu and Bianmi syndromes were improved, the FBDT was significantly shortened (<0.05, <0.01), the water content of colon was increased in the three groups of Danggui decoction (<0.05, <0.01). The water content of the stool was obviouslyincreased in groups of 16.67and 8.33kg.dDanggui decoction (<0.05). The atrophy of colon mucosa and gland was improved, the mucus secretion was increased, and the colon lubrication function was improved in the three groups in different doses of Danggui decoction.@*CONCLUSIONS@#Dangguican improve the mucosal atrophy, and increase the secretion of colonic mucus, then the stool is softened and promoted to discharge.

Histopathological lesions in gill of air-breathing cat fish mystus cavasius exposed to of electroplating industrial effluent nickel.

The gill which participate in many important functions in fish, such as respiration, osmoregulation and excretion. Electroplating industrial effluent nickel induced hyperplasia, multiple telangiectases (aneurysms), desquamation of the epithelial cells, complete fusion of secondary gill lamellae and congestion of blood sinuses were the significant histopathological lesions observed in the gill of Mystus cavasius.

EFFECT OF THE CHANGES IN MICROTUBULAR-MICROFILAMENTOUS SYSTEM ON THE MUCUS SECRETION OF CULTURED MGc8e-3 HUMAN STOMACH CANCER CELLS / 解剖学报

cells was not influenced if they were treated with 1(T~(-5)M taxol and 0.1?g/ml colcemid for 2 hrs. either simultaneously or in accession. The secretory activity of these cells was evidently inhibited after treatment with 4?g/ml cytochalasin B for 6 hrs. Our results indicate that the mucus secretion of MGc80-3 human stomach cancer cells closely depended on the presence of both microtubules and microfilaments. We also found that the mucus secretion of MGc80-3 cells was enhanced by treatment with l?g/ml pilocarpine for 6 hrs. The proliferation of MGc80-3 cell population was inhibited by thiazolidine-4 carboxylic acid. However, the secretory activity of these cells was evidently enhanced after treatment with 0.1?g/ml thiazolidine-4 carboxylic acid for 10 hrs. The stimulating effect of pilocarpine and thiazolidine-4 carboxylic acid disappeared after the microtubules and microfilaments were disassembled by treatment with colcemid and cytochalasin B. In addition, our experiments also show that the mucus secretion of these cells was increased after treatment with ImM db-cAMP. These findings convincingly support the assumption that motile events placed under the control of the microtubular-microfilamentous system are intimately involved in the mucus secretion of MGc80-3 human stomach cancer cells. cells was not influenced if they were treated with 1(T~(-5)M taxol and 0.1?g/ml colcemid for 2 hrs. either simultaneously or in accession. The secretory activity of these cells was evidently inhibited after treatment with 4?g/ml cytochalasin B for 6 hrs. Our results indicate that the mucus secretion of MGc80-3 human stomach cancer cells closely depended on the presence of both microtubules and microfilaments. We also found that the mucus secretion of MGc80-3 cells was enhanced by treatment with l?g/ml pilocarpine for 6 hrs. The proliferation of MGc80-3 cell population was inhibited by thiazolidine-4 carboxylic acid. However, the secretory activity of these cells was evidently enhanced after treatment with 0.1?g/ml thiazolidine-4 carboxylic acid for 10 hrs. The stimulating effect of pilocarpine and thiazolidine-4 carboxylic acid disappeared after the microtubules and microfilaments were disassembled by treatment with colcemid and cytochalasin B. In addition, our experiments also show that the mucus secretion of these cells was increased after treatment with ImM db-cAMP. These findings convincingly support the assumption that motile events placed under the control of the microtubular-microfilamentous system are intimately involved in the mucus secretion of MGc80-3 human stomach cancer cells.