RESUMO
Gastric cancer is a common malignant tumor in China. The treatment of gastric cancer has entered the era of immunotherapy. Multiplex immunofluorescence technology is a new detection technology in recent years, including multiplex immunofluorescence staining, multispectral imaging and tissue quantitative analysis, which can co-locate specific immune cell populations in tumor immune microenvironment. A large number of studies used multiplex immunofluorescence technology to detect different cell subsets and their molecular expression in the immune microenvironment of gastric cancer, which had shown important clinical value in the treatment and prognosis evaluation of gastric cancer. This technique has certain limitation, but it has much advantages, such as high repeatability, high efficiency and high cost-effectiveness compared with traditional methods. This article reviewed the clinical application of multiplex immunofluorescence technique in immunotherapy of gastric cancer.