Actividad cicatrizante de seis extractos hidroalcohólicos de plantas en heridas incisas de Rattus norvegicus albinus / Healing activity of six hydroalcoholic extracts of plants in incised wounds of Rattus norvegicus albinus

Introducción: En la medicina militar, los agentes cicatrizantes naturales frente a heridas por incisión son relevantes en el tratamiento de los militares. Objetivo: Evaluar la actividad cicatrizante de los extractos hidroalcohólicos de Peperomia congona Sodiro (congona), Annona muricata L. (guanábana), Urtica urens L. (ortiga),Ormosia coccinea (Aubl) Jacks (huayruro), Opuntia ficus-indica L. Mill. (tuna) y Musa acuminata Colla (plátano bellaco) en heridas incisas de Rattus norvegicus albinus (rata albina). Métodos: Estudio analítico experimental de tipo prospectivo y longitudinal. Se realizó el análisis fitoquímico preliminar de los 6 extractos. Se emplearon 80 ratas albinas machos, aleatorizadas y distribuidas en 8 grupos (n = 10). Se realizó una aplicación dermal durante 10 días consecutivos posteriores a la generación de heridas incisas que se realizaron en el dorso. Grupo I (cloruro de sodio al 0,9 por ciento). Grupo II (gel cicatrizante comercial). Del Grupo III al VIII fueron tratados con extracto hidroalcohólico de congona, guanábana, ortiga, huairuro, tuna y plátano bellaco respectivamente. La evolución de la cicatrización fue seguida en los días 1, 5 y 11 y medida en milímetros. Resultados: Se detectó la presencia de alcaloides, compuestos fenólicos y flavonoides en los 6 extractos; además de cumarinas en congona, taninos en guanábana, ortiga, tuna y huayruro, y quinonas en guanábana y plátano bellaco. Se comprobó la actividad cicatrizante en los extractos de guanábana (69,77 por ciento), tuna (66,27 por ciento), plátano bellaco (64,38 por ciento), ortiga (56,73 por ciento), congona (55,74 por ciento) y huayruro (54,50 por ciento), comparados con un gel comercial (72,21 por ciento). Conclusiones: El extracto hidroalcohólico de las hojas de Annona muricata L. (guanábana) presentó la mayor actividad cicatrizante en heridas incisas de ratas albinas machos(AU)

Introduction: In military medicine, natural healing agents against incision wounds are relevant in the treatment of the military. Objective: To evaluate the healing activity of the hydroalcoholic extracts of Peperomia congona Sodiro (congona), Annona muricata L. (guanabana), Urtica urens L. (ortiga), Ormosia coccinea (Aubl) Jacks (huairuro), Opuntia ficus-indica L. Mill. (tuna) and Musa acuminata Colla (platano bellaco) in incised wounds of Rattus norvegicus albinus (albino rat). Methods: Experimental analytical study of prospective and longitudinal type. The preliminary phytochemical analysis of the 6 extracts was carried out. We used 80 male albino rats, randomized and distributed in 8 groups (n = 10). A dermal application was made during 10 consecutive days after the generation of incised wounds that were made on the back. Group I (0.9 percent sodium chloride). Group II (commercial healing gel). From Group III to VIII they were treated with hydroalcoholic extract of congona, guanábana, ortiga, huairuro, tuna and platano bellaco respectively. The evolution of healing was followed on days 1, 5 and 11 and measured in millimeters. Results: The presence of alkaloids, phenolic compounds and flavonoids was detected in the 6 extracts; in addition to coumarins in congona, tannins in guanábana, nettle, tuna and huayruro, quinonas in guanábana and plantain bellaco. The healing activity was verified in the extracts of guanabana (69.77 percent), tuna (66.27 percent), platano bellaco (64.38 percent), ortiga (56.73 percent), congona (55.74 percent) and huayruro (54.50 percent), compared with a commercial gel (72.21 percent). Conclusions: The hydroalcoholic extract of the leaves of Annona muricata L. (guanábana) showed the highest healing activity in incised wounds of male albino rats(AU)

Formulation and evaluation of semisolid jelly produced by Musa acuminata Colla (AAA Group) peels

Objective: To study the jelly formulation produced by Musa acuminata Colla (AAA Group) peels and evaluate its antioxidant properties which are related to the product quality. Methods: The formulations of peel jelly were established under two-level full factorial designs within two blocks and one center point. Regarding response optimizer, the amount of sugar and citric acid was obtained; hence, the peel jellies were produced. The evaluation of antioxidant properties was conducted by using total phenolic content (TPC) assay and 1,1 diphenyl-2-picrylhydrazyl (DPPH) free radical assay. Results: The TPC of peel powder varied from 91.8 to 602.26 mg gallic acid equivalents/100 g dry weight, and 5%–7% peel jellies had phenolic content ranging from 29.38 to 48.31 mg gallic acid equivalents/100 g dry weight. The results of DPPH test indicated that at 10 mg/mL, the peel powder showed 89% DPPH inhibition, while 7% peel jelly prominently exhibited 84% DPPH inhibition. The correlation between DPPH IC50 value and TPC of peel powder as well as peel jelly was quite reasonably high with correlation coefficient ranging from 0.843 7 to 0.995. Conclusions: TPC can be used as an indicator in assessing the antioxidant activity of fruits and vegetables. The present investigation reveals that TPC is mainly responsible for DPPH free radical scavenging capacity.

Formulation and evaluation of semisolid jelly produced by Musa acuminata Colla (AAA Group) peels

Objective: To study the jelly formulation produced by Musa acuminata Colla (AAA Group) peels and evaluate its antioxidant properties which are related to the product quality. Methods: The formulations of peel jelly were established under two-level full factorial designs within two blocks and one center point. Regarding response optimizer, the amount of sugar and citric acid was obtained; hence, the peel jellies were produced. The evaluation of antioxidant properties was conducted by using total phenolic content (TPC) assay and 1,1 diphenyl-2-picrylhydrazyl (DPPH) free radical assay. Results: The TPC of peel powder varied from 91.8 to 602.26 mg gallic acid equivalents/100 g dry weight, and 5%-7% peel jellies had phenolic content ranging from 29.38 to 48.31 mg gallic acid equivalents/100 g dry weight. The results of DPPH test indicated that at 10 mg/mL, the peel powder showed 89% DPPH inhibition, while 7% peel jelly prominently exhibited 84% DPPH inhibition. The correlation between DPPH IC