Analysis of enzymological characterization of N-V proteinase with fibrinolytic activity / 吉林大学学报(医学版)

Objective To detect the optimum reaction temperature,pH,Km of N-V proteinase and determine its enzymological classification.Methods The fibrin plate method was used to determine the optimum reaction temperature and pH of N-V proteinase.The Km of N-V proteinase was detected by using Leu-pNA as substrate.E64,PMSF,zymofren and 1,10-phenanthroline were used as inhibitors to determine proteinase classification of N-V proteinase.Results The optimum reaction temperature was 50℃,pH was 8-9,Km was 1.97?10-3mol?L-1.PSMF inhibited the fibrinolytic activity of N-V proteinase(P0.05).Conclusion N-V proteinase belongs to serine proteinase.