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【Objective】To observe the dynamic changes of flavonoids contents of Citrus grandis(L.)Osbeck var.tomentosa Hort..【Methods】The total flavonoid content was determined by spectral photometric analysis and naringin content by HPLC,and the fingerprints of Citrus grandis(L.)Osbeck var.tomentosa Hort.were studied by HPLC.【Results】With the increase of the fruit age,total flavonoid contents in the peel and the leaves of Citrus grandis(L.)Osbeck var.tomentosa Hort.decreased obviously.Fingerprints results showed that at the early fruit age the rhoifolin content in the peel increased with the fruit age and began to decrease 62 days later,while the rhoifolin content in the leaves showed no changes.【Conclusion】In terms of active components contents and economic value,the optimal collecting time for Citrus grandis(L.)Osbeck var.tomentosa Hort.is:young fruit,34 days,and immature fruit,55 days.
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[Objective] To assess the quality of Citrus grandis Osbeck. Var. tomentosa Hort. (CGO Var. TH) and Citrus grandis (L. ) Osbeck (CGO). [Methods] The macroscopic feature and microscopic structure of CGO Var. TH and CGO were compared. Their content of total flavones (TF) was determined by spectrometry, naringin content and fingerprint by high performance liquid chromatography (HPLC) and the content of rhoifolin by thin-layer chromatography (TLC). Their expectorart, anti-inflammatory and antitussive effects were observed by phenol red test, auricular swelling test and ammonia-water-induced cough method. [Results] Obvious differences such as villi in the exocarp and non-grandular hair in the stem existed in the macroscopic feature and microscopic structure of CGO Var. TH and CGO. The contents o{ total flavones and naringin in CGO Var. TH were markedly higher than that in CGO and the rhoifolin content in CGO Var. TH was ten times as much as that in CGO. Markedly difference was also found in their fingerprint. The expectorant, anti-inflammatory and antitussive effects in CGO Var. TH were better than those in CGO. [Conclusion] The quality of CGO Var. TH is much better than that of CGO.
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[Objective] To establish a determination method for naringin in Xingsu Ke Oral Liquid by high performance liquid chromatography (HPLC) . [ Methods ] At room temperature, HPLC was performed on ODS column; the mobile phase consisted of acetonitrile and 8% acetic acid (15: 85) and the detection wavelength was at 283nm. [Results] The linearity of naringin was good in the range of 1.6 - 9.6 mg/L ( r = 0.999 9), the average recovery was 98.62 % and RSD = 0.754 ( n = 5). [ Conclusion ] This method is simple and effective and can be used for the quality control of Xingsu Ke Oral Liquid.
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Objective To explore the genuineness of Exocarpium Citri Grandis(ECG) from Huazhou city of Guangdong province.Methods We used the method of high performance liquid chromatography to detect the naringin content in ECG from different producing areas of Huazhou city.Random amplified polymorphic DNA analysis was used for the examination of genetic distance,and plasma-atomic emission spectrometry for the detection of soil elemental abundance of 8 elements such as aluminium(Al),kalium(K),calcium(Ca),ferrum(Fe),titanium(Ti),boron(B),magnesium(Mg),and manganese(Ma).The correlation of the above three parameters was analyzed by statistical software SPSS 11.5.Results Ca abundance in the surface soil layer had an obvious effect on the content of naringin,and the difference of Al and K abundance in subsoil layer was correlated with the genetic distance of ECG.Conclusion The genuineness of ECG is probably related with the abundance of phlopopitum in the soil of producing areas of Huazhou city.
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Objective To establish a method for the determination of naringin in Gongning Granule, Methods The sample was extracted with methyl and the chromatographic conditions were as follows: Kromasil C18 chromatographic column(250 mm?4.6 mm ,5 ?m)with a mobile phase of methyl and 1 %acetic acid (35.5 ∶64.5),the detection wavelength at 283 nm and the flow rate being 1.0mL?min-1. Results A linearity of naringin in Gongning Granule was obtained in the range of 0.0980 ?g to 0.980 ?g,r=0.9997 (n=6).The average recovery was 98.7 %and RSD=1.96 %(n=6). Conclusion This method is easy,sensitive and accurate for the determination of naringin in Gongning Granule.