Separation and Purification of Polysaccharides Sulfate from Ulva lactuca and Its Structure Analysis / 中国药房

OBJECTIVE:To study the separation and purification of polysaccharides sulfate from Ulva lactuca,and to analyze its structure.METHODS:The separation and purification method of polysaccharides sulfate from U.lactuca was optimized.The content of polysaccharides in polysaccharides sulfate was determined by phenyl-sulfuric acid method.The molecular amount of polysaccharides was determined by HPGC.The structure of polysaccharides was analyzed by IR and 13C-NMR.RESULTS:The optimal extraction condition of polysaccharides sulfate from U.lactuca were crashed materials 20 mesh sieve,20-fold water,decocting 3 times at 85 ℃,2 hours each time.The content of polysaccharides was 22.64% and molecular amount was 1 156 112.Soluble polysaccharides mainly contained ?-glucuronic acid,rhamnose and plenty of sulfate radical.CONCLUSION:The method is simple and stable,which provides theoretic basis for production of polysaccharides sulfate from U.lactuca.

Studies on structural features of two neutral heteropolysaccharides from Cuscuta chinensis / 中草药

Object Polysaccharide constituents in the seed of Cuscuta chinensis Lam were investigated Methods Individual constituent was isolated and purified by DEAE cellulose and gel filtration chromatography, and its structural features were further studied by physicochemical constants, and spectral analysis Results Two polysaccharides, named H6 and H8, were obtained from alkalescent or boiling water extract fractions, respectively The molecular mass of H6 was 3 14?10 5 and that of H8 was more than 1 0?10 6 Both structures were found to be complex neutral heteropolysaccharides composed of Ara, Rha, Gal and Xyl Conclusion These polysaccharides were obtained from this plant for the first time

A new quinolone alkaloid with antibacterial activity from Lappula echinata / 中草药

Objective To investigate the chemical constituents of Lappula echinata and determine the antibacterial activity.Methods A new quinolone alkaloid was isolated from the BuOH extract of L.echinata by silica gel column chromatography and gel column chromatography.Its structure was identified by 1H-NMR, 13C-NMR, 2D-NMR, HR-MS, UV, and IR spectral data analysis.Its antibacterial activity was determined by KB method.Results A new quinoloe alkaloid named 8-methoxy-4-quinolone-2-caboxylic acid was isolated from L.echinata and was found to have antibacterial activity on Pseudomonas pyocyanea ATCC 27853, EPEC O111, pneumobacillus and Staphylococcus epidermidis.Conclusion This is a new compound with antibacterial activity.