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The release of Guidelines to Strengthen the Governance over Ethics in Science and Technology marked that China's ethical governance system of science and technology has entered a new stage.The research and practice on ethics in science and technology in China are carried out along two approaches.One approach is to derive the rules of specific science and practice from the principles of general ethics.The other is to gradually form ethical governance principles and consensus for a specific type of phenomenon or problem in the process of science and technology,starting from scientific research problems and scientific and technological events and cases.Medical ethics research has also formed the tradition of medical ethics and modern bioethics in this way.In the practice of ethical governance,medical ethics is at the forefront of scientific and technological ethics,forming a"pilot area"for the research and development of scientific and technological ethics,an"explorer"for the institutionalization of ethical review,and a"demonstration area"for scientific and technological ethical governance.The release of ethical governance opinions is not the end of research on medical ethics governance,and medical ethics needs to have new achievements.Firstly,rethinking the theoretical basis and methodology of medical ethics and adhering to the guidance of Marxist theory.Secondly,a rethinking of the two research paths and how they can balance development and support each other.Thirdly,rethinking the relationship between ethics and the rule of law,and continuously improving the level of legalization of ethical governance.
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This research investigated psychological correlates with norm non-compliance during the National State of Emergency declared in Peru at the time of the COVID-19 pandemic. Specifically, it looks at the relationship between Social Dominance Orientation (SDO), perceptions of legitimacy, strength and efficacy of the normative system, and trust in the institutions. It also looks at individual justifications for norm non-compliance and the frequency of transgression. A correlational research design using questionnaires was developed with a sample of 126 participants. Results show that transgression of government-issued norms during the Peruvian National State of Emergency is linked to the ideological compo- nent of SDO and perceptions of a weak and illegitimate normative system. These results are consistent with previous studies on normative transgression in Latin American countries. Additionally, results suggest that the need for subsistence (i.e., generating resources to sustain the household) is fundamental to comprehensively understanding transgressive behavior in contexts of vulnerability such as a global pandemic. The discussion highlights how a precarious socio-political structure one incapable of meeting the basic needs of large sectors of the population emerges as a central cause of normative transgression in times of crisis
Esta investigación indaga los correlatos psicológicos con el incumplimiento de normas durante el Estado de Emergencia Nacional declarado en Perú con ocasión de la pandemia del COVID-19. Específicamente, se exa- mina la relación entre la Orientación a la Dominancia Social (ODS), las percepciones de legitimidad, fortaleza y eficacia del sistema normativo y la confianza en las instituciones. También examina las justificaciones individuales del incumplimiento de las normas, así como la frecuencia de la transgresión. Se desarrolló un diseño de investigación correlacional mediante cuestionarios con una muestra de 126 participantes. Los resultados muestran que la transgresión de las normas emitidas por el Gobierno durante el Estado de Emergencia Nacional peruano, están vinculadas al componente ideológico del ODS y a la percepción de un sistema normativo débil e ilegítimo. Estos resultados son consistentes con estudios previos sobre transgresión normativa en países latinoamericanos. Adicionalmente, los resultados sugieren que la necesidad de subsistencia (es decir, la generación de recursos para el sostenimiento del hogar), es fundamental para comprender de manera integral el comportamiento transgresor en contextos de vulnerabilidad como una pandemia global. La discusión destaca cómo una estructura sociopolítica precaria incapaz de satisfacer las necesidades básicas de amplios sectores de la población emerge como causa central de la transgresión normativa en tiempos de crisis.
Esta pesquisa investiga as correlações psicológicas com o descumprimento de normas durante o Estado Nacional de Emergência declarado no Peru na época da pandemia da COVID-19. Especificamente, ela analisa a relação entre a orientação de dominância social (ODS), as percepções de legitimidade, força e eficácia do sistema normativo e a confiança nas instituições. Também analisa as justificativas individuais para a não conformidade com a norma, bem como a frequência da transgressão. Um projeto de pesquisa correlacional usando questionários foi desenvolvido com uma amostra de 126 participantes. Os resultados mostram que a transgressão das normas emitidas pelo governo durante o Estado de Emergência Nacional do Peru está ligada ao componente ideológico da ODS e às percepções de um sistema normativo fraco e ilegítimo. Esses resultados são consistentes com estudos anteriores sobre transgressão normativa em países latino-americanos. Além disso, os resultados sugerem que a necessidade de subsistência (ou seja, a geração de recursos para sustentar a família) é fundamental para compreender de forma abrangente o comportamento transgressivo em contextos de vulnerabilidade, como uma pandemia global. A discussão destaca como uma estrutura socio- política precária incapaz de atender às necessidades básicas de grandes setores da população surge como uma causa central da transgressão normativa em tempos de crise.
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HumanosRESUMO
Abstract BACKGROUND Stigmatization, which emerges depending on the sexual behavior of young individuals, leads to negative health and social outcomes, such as shame, social marginalization, violence, and mental health morbidity. Objective: This study aimed to examine the correlation between the level of sexual and reproductive health stigma and gender perception in female university students. DESIGN AND SETTING: This descriptive cross-sectional study was conducted at the Faculty of Health Sciences of a university in Turkey. METHODS: The data of this study were collected from digital media between July and October 2020 from a study population of 385 students. The data were collected using the Personal Information Form, including the socio-demographic characteristics of students, the Sexual and Reproductive Health Stigmatization Scale in Young Women and the Perception of Gender Scale. Descriptive statistics, independent samples t-test, analysis of variance, and Pearson's correlation test were used to assess the data. RESULTS: It was determined that there was a negative correlation between the Sexual and Reproductive Health Stigmatization Scale in Young Women and the Perception of Gender Scale (r = -0.173, P = 0.001). CONCLUSION: It was determined that as the gender perception in the young women who participated in the study increased, the sexual and reproductive health stigmatization level decreased. The sexual and reproductive health stigmatization levels of the participants were at an above average level, and gender perception was at a medium level.
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Objective To establish the norm of Sub-Health Measurement Scale for nurses in Shandong Province,so as to provide a basis for understanding the current situation of sub-health and provide a reference for promoting the health of nurses.Methods From December 2020 to February 2023,14,308 nurses from 23 public tertiary hospitals enrolled in the Nurse Health Cohort Study in Shandong Province were selected as participants by convenience sampling.The sub-health status was assessed by the Sub-Health Measurement Scale,and the sub-health norm of nurses was constructed by establishing the mean,percentile and demarcation norm.Results A total of 10669 nurses were included in the analysis.The total score of Sub-Health Measurement Scale of nurses in Shandong Province was(61.13±12.40),and the scores of physiological sub-health sub-scale,psychological sub-health sub-scale and social sub-health sub-scale were(58.35±11.86),(62.56±15.85)and(63.56±16.73),respectively.The mean norm of sub-health assessment scale was established by gender and age.Based on the results of univariate analysis,we selected the characteristics with differences,namely gender,age,marital status,working department,years of working,professional title and employment type to establish a classification norm.P5,P25,P50,P75 and P95 were select to establish percentile norm.Taking(x-s),(x-0.5s),(x+0.5s),(x+s)as the cut-off points,the demarcation norm of the Sub-Health Measurement Scale and sub-scales was established,which was divided into 5 categories,including disease,severe sub-health,moderate sub-health,mild sub-health and healthy states.Conclusion This study established the Sub-Health Measurement Scale norm of nurses,which provided a basis for effectively evaluating and identifying the sub-health status of nurses.
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Objective:To investigate the self-measured health status of college students in Tibet, and to construct the self-measured health scale (SRHMS V1.0) norm of College students in Tibet.Methods:This was a cross-sectional study. A multistage stratified sampling method was used. From April to June 2022, a total of 7 990 college students were selected from all colleges and universities in the Tibet Autonomous Region (7 in total). The self-rated health of Tibetan college students was investigated and evaluated by combining demographic information and SRHMS V1.0. Descriptive statistics, t-test, analysis of variance ( Brown-Forsythe test for unequal variance, LSD test for multiple comparisons), and Spearman correlation analysis were used to construct mean norm, percentile norm, and delimitation norm of physiological scale health (PSH), mental scale health (MSH), social scale health (SSH) score and total scale health (TSH) scores. Results:The total score of self-assessed health assessment among college students in Tibetan universities was (72.18±12.35). For different genders, the PSH, MSH, SSH and TSH scores were (73.85±13.78), (65.80±14.73), (69.85±16.00) and (73.44±12.77) for boys and (71.18±13.36), (62.81±14.03), (68.57±14.90) and (70.92±11.94) for girls, respectively. Scores on each subscale and total scale were statistically significant different between the different sexes ( t=2.531, 2.672, 1.867, 2.623, all P<0.05). For different grades, the PSH, MSH, SSH and TSH scores of the freshman were (73.36±13.23), (65.77±14.58), (70.98±15.60) and (73.51±11.91); the sophomore were (70.74±13.73), (62.40±13.60), (66.92±14.62) and (70.16±12.28), the junior were (75.48±13.09), (64.08±15.12), (71.90±15.12) and (74.10±12.36); and the senior were (67.21±14.41), (59.19±17.67), (64.91±18.59) and (66.94±14.59) respectively, with the differences in scores of each subscale and the total scale in different grades being statistically significant ( F=3.952, 3.611, 4.841, 5.583, all P<0.05). The mean norm, percentile norm and demarcation norm of the total score and each subscale of self-measured health of college students in Tibetan universities were constructed with gender and grade as the cut-off values. Conclusion:The model of self-measured health assessment scale for students in colleges and universities in Tibet is established, which can provide evaluation criteria for evaluating the health status of college students in Tibet and plateau areas.
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{L-End}Objective To explore the suitable methods for individual occupational stress examination and evaluation for workers in China based on the electronics industry. {L-End}Methods A total of 1 164 workers from four electronics enterprises in Beijing City were selected as the research subjects using a convenient sampling method. The Occupational Stress Measurement Scale, which was developed based on the Japanese Brief Job Stress Questionnaire, was used to assess the occupational stress of the research subjects, and test the reliability and validity of the scale. Percentile norms and T-score norms were established, and the T-scores of the three dimensions of stress reaction (psychological reaction and physical symptoms), stress factors, and social support were divided into five stages using the normal distribution method with x¯±0.5 s and x¯±1.5 s, which was used to explore a proposed standard for assessing individual stress levels. {L-End}Results The revised scale consisted of 57 items, which could explain 64.0% of the total variation of occupational stress based on the result of item analysis and exploratory factor analysis. The results of confirmatory factor analysis showed that the model fitted well, with root mean square error of approximation of 0.052, standardized root mean square residual of 0.070, comparative fit index of 0.960, and Tucker-Lewis index of 0.958. The Cronbach's α coefficient of the scale was 0.951, and the Spearman-Brown coefficient was 0.837. Cronbach's α coefficients of the three dimensions ranged from 0.794 to 0.952, and the Spearman-Brown coefficients ranged from 0.737 to 0.850. The scores of the three dimensions of stress reaction, stress factors, and social support were (49.1±13.0), (44.9±7.7), and (18.1±3.6), respectively. Workers in the electronics industry met one of the following items were identified as the high level of occupational stress individuals: i) a score of stress reaction dimension ≥72.0 points; ii) a sum of stress factor and social support dimension scores ≥81.0 points, and a stress reaction dimension score ≥58.0 points. {L-End}Conclusion The Occupational Stress Measurement Scale and the criteria for determining high level of occupational stress can be used to assess the individual occupational stress levels of workers in the electronics industry in China. This method can provide a theoretical basis for the formulation of occupational stress examination and assessment for Chinese workers.
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{L-End}Objective To establish the norm of Core Occupational Stress Scale (COSS) for electronic manufacturing industry workers in China. {L-End}Methods A total of 3 049 workers from five electronic manufacturing enterprises in four prefecture-level cities concentrated distribution of the electronics manufacturing industry in China were selected as research subjects using a stratified sampling method. COSS was used to investigate occupational stress levels, and the mean norm, percentile norm and threshold norms were established. {L-End}Results The average score of COSS for the electronic manufacturing industry workers in China was (43.5±7.4) points, and the average scores of social support, organization and reward, demand and effort, and autonomy dimensions were (9.5±3.1), (15.1±3.9), (13.1±3.0), and (5.7±2.0) points, respectively. A total score of 0.0-<47.0 points was determined as no occupational stress, 47.0-<51.0 points as mild occupational stress, 51.0-≤54.0 points as moderate occupational stress, and >54.0 points as severe occupational stress. {L-End}Conclusion The norm of COSS for workers in China's electronics manufacturing industry has been established, which can provide a reference for the evaluation and intervention of their occupational stress levels.
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Virtue ethics and normative ethics are corresponding ethical concepts, and their collision and integration promote the development of ethical system. On the issue of doctor-patient relationship, from virtue to norm is the objective requirement of social transformation and development, and is also the inevitable choice to resolve doctor-patient conflicts and reshape doctor-patient relationships. In the new era, the construction of doctor-patient relationship faces ethical dilemma such as one-sided emphasis on doctors’ virtue cultivation, unclear ethical responsibilities between doctors and patients, and lack of trust and tolerance between doctors and patients. Therefore, it is necessary to strengthen the institutionalization of doctor ethics, clarify the ethical responsibilities of both doctors and patients, optimize the social ethical ecological environment, so as to realize the ethical reconstruction of doctor-patient relationship from virtue to norm.
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ABSTRACT Oil and gas, mining, among others, are examples of facilities where naturally occurring radioactive materials can be found. This study aims to evaluate the presence of natural radioactive series, especially those of 238U and 232Th, in the water treatment plants of Poços de Caldas City, Minas Gerais. The presence of these series was investigated in samples of raw water, treated water, sludge from decanters, and scale from Parshall gutters. The sludge, input, and scale samples were submitted to the gamma spectrometry technique to determine the 226Ra, 228Ra, and 210Pb radionuclides. For U and Th, ultraviolet visible spectrophotometry was performed, and for the alpha and beta total values, radiochemical separation and subsequent alpha and beta total counts were performed. The results indicate that water samples are within the Ministry of Health Ordinance n° 5 (2017). Due to the different concentrations of radionuclide activity in the sludge, it was not possible to affirm the same order of magnitude with the sediment from the catchments. However, the values are in accordance with those established by the European Union Council for Naturally-Occurring Radioactive Materials. In the scale, the contents of 1192, 1704, and 301 Bq kg−1 were identified for 226Ra, 228Ra, and 210Pb, respectively. In the inputs of aluminum sulfate and calcium hydroxide, no relevant activities were identified. The results obtained in the study can serve as an indicative regarding the need for a more detailed evaluation of the radiological issue in question concerning public water supplies.
RESUMO Petróleo e gás, mineração, estações de tratamento de água, entre outros, são exemplos de instalações que podem apresentar Materiais Radioativos de Ocorrência Natural. Neste estudo, objetivou-se avaliar a presença de séries radioativas naturais, especialmente as de 238U e 232Th nas estações de tratamento de água da cidade de Poços de Caldas/MG. Foram investigadas as presenças dessas séries em amostras de água bruta, de água tratada, no lodo dos decantadores e nas incrustações das calhas Parshall, além dos principais insumos utilizados. As amostras de lodo, insumos e incrustações foram submetidas à técnica de espectrometria gama para a determinação dos radionuclídeos 226Ra, 228Ra e 210Pb. Para U e Th, realizou-se espectrofotometria ultravioleta-visível, e para os valores de Alfa e Beta totais foram realizadas separação radioquímica e posterior contagem Alfa e Beta total. Os resultados indicaram que as amostras de águas estão em conformidade com a Portaria de Consolidação n° 5 de 2017 do Ministério da Saúde. Dadas as diferentes concentrações de atividade dos radionuclídeos no lodo, não foi possível afirmar a mesma ordem de magnitude com o sedimento das captações. Entretanto, os valores estão consonantes com o estabelecido pelo conselho da União Europeia para Materiais Radioativos de Ocorrência Natural. Nas incrustações foram identificados teores de 1.192 Bq.kg-1, 1.704 Bq.kg-1 e 301 Bq.kg-1 para 226Ra, 228Ra e 210Pb, respetivamente. Já para os insumos Sulfato de Alumínio (Al4(SO4)3) e Hidróxido de Cálcio Ca(OH)2 não foram identificadas atividades relevantes. Os resultados obtidos no estudo podem servir como indicativos da necessidade de uma avaliação mais detalhada sobre a questão radiológica em foco, em relação ao abastecimento público de águas.
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Objective:To construct a geriatric comprehensive health evaluation system, and establish the norm of corresponding score.Methods:The methods of Delphi survey was implemented to develop the geriatric comprehensive health evaluation system.The weight coefficients of the items were calculated and assigned according to the expert-graded importance score and Saaty analytic hierarchy process.Reliability and validity of the system were examined.Goodness of fit was evaluated by confirmatory factor analysis.The demarcation norm of geriatric comprehensive health score was established based on an empirical study of 2, 118 non-hospitalized elderly population.Results:During the process of Delphi expert consultation and item analysis, nine items were deleted and two items were added.In the end, the 29-item comprehensive geriatric health evaluation system was developed, which included three dimensions of physiology, psychology, as well as social support and environment.The Cronbach's α coefficient of the evaluation system was 0.709.The exploratory factor analysis-extracted ten common factors meet ten second-level items.Confirmatory factor analysis indicated that the goodness of fit of the system was good.Geriatric health status was divided into five grades of very poor, poor, general, good and excellent by 0.5 to 1 standard deviation.The demarcation norm of geriatric comprehensive health score for different age and gender was established accordingly.Conclusions:The reliability and validity of the geriatric comprehensive health evaluation system are satisfactory.The construction of comprehensive health evaluation system and the establishment of the norm can facilitate the quantitative assessment of geriatric health status, and provide a reference for the screening and grading of individual health status in the elderly.
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Objective:To establish the norm of clinical nursing teachers' competency in tertiary first-class hospitals in northwest China.Methods:From August 2019 to February 2020, 1 077 clinical nursing teachers were selected from 12 tertiary first-class hospitals in northwest China by stratified, random and proportional sampling, and were investigated with the Clinical Nursing Teacher Competency Evaluation Scale. Descriptive statistics were carried out on the sample data, and univariate variance analysis and pairwise comparison were carried out on the multi-classification variables of age, nursing age, professional title, teaching years and the number of teachers per year. The verified data were exported to SPSS 26.0 for statistical analysis, variance analysis was used for multi-group comparison, and LSD test was used for pairwise comparison between groups.Results:①The corresponding mean norm was established by grouping the total score and the first-class index; the percentile norm was made with 5% spacing; P10, P30, P70 and P90 were selected as the grading demarcation points, and the demarcation norm was constructed. ②Factor analysis showed that there were statistical differences in personality characteristics among different ages, nursing ages and professional titles ( P<0.05), and also in professional attitudes among different nursing ages and professional titles ( P<0.05). Conclusion:The establishment of norm provides references for individuals or units to evaluate teachers' competence.
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@#China has a huge cumulative storage of NORM solid waste, which urgently needs to be treated and disposed of in a scientific and standard way. Through analyzing the current status and technologies of treatment and disposal of NORM solid waste and relevant environmental radiation protection regulations and standards, we put forward suggestions on the improvement of regulations and standards and the applicability of treatment and disposal technologies, to provide a reference for the green and sustainable development of mineral resources in China.
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This study aimed to adapt and raise evidences of validity based on the internal structure, on the relationship with other variables, and on the content of the Fear of COVID-19 Scale (FCV-19S) in Brazilian Portuguese. We performed the Confirmatory Factor Analysis of the scale, its invariance analysis by gender, and established norms for interpreting the instrument's scores. Participants were 1,000 adults of both genders. The findings showed all fit indices as satisfactory, confirming the scale's one-dimensionality and its invariance. Results also demonstrated convergent validity between the FCV-19S and the stress of the subjects. Moreover, the stratification of the intensity of fear (mild, moderate and severe) was determined based on the standardization of scores. We concluded that FCV-19S presents sufficient evidence to support its use to assess the fear of Covid-19S in Brazil.
Este estudo objetivou adaptar e levantar evidências de validade com base na estrutura interna, na relação com outras variáveis e de conteúdo da Escala de Medo da COVID-19 (EMC-19, Fear of COVID-19 Scale) para português brasileiro. Para tanto, realizou-se a Análise Fatorial Confirmatória da medida e sua análise de invariância por sexo; ademais, estabeleceu--se normas para interpretação dos escores do instrumento. Fizeram parte da pesquisa 1.000 adultos, de ambos os sexos. Os resultados evidenciaram que todos os índices de ajuste foram satisfatórios, confirmando a unidimensionalidade da escala, bem como sua invariância. Constatou-se, ainda, que houve validade convergente entre a EMC-19 e o estresse dos participantes. Além disso, foram propostos estratos de classificação da intensidade do medo (leve, moderado e severo) com base na normatização dos escores. Por fim, concluiu-se que a EMC-19 apresenta evidências suficientes que recomendam a sua utilização para medida do medo da COVID-19 no Brasil.
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Análise Fatorial , Coronavirus , MedoRESUMO
Abstract Background: Buffalo breeding has significantly increased in Brazil over recent years. However, few genetic evaluations have been conducted. Objective: To assess Genotype x Environment Interactions in the Mediterranean Water Buffalo in Brazil, for weight at 205 days of age, using reaction norm models via random regression. Methods: Data for buffaloes born between 1990 and 2014 were collected from five farms ascribed to the Brazilian Buffaloe Improvement Program, located in the North (1), Northeast (1), South (2) and Southeast (1) regions of Brazil. The initial database consisted of 5,280 observations at 205 days of age (W205). We assessed fit using two hierarchical reaction norm models: a two-step (HRNM2s) and a one-step (HRNM1s). Model fit was estimated using the Deviance Information Criterion, Deviance Based on Bayes Factors and Deviance based on Conditional Predictive Ordinate. The environmental descriptors were created to group individuals into common production environments based on year, season, herd and sex. Results: The best fit was obtained for the hierarchical reaction norm model with one-step (HRNM1s). Direct heritability estimates for this model ranged from 0.17 to 0.67 and the maternal heritability from 0.02 to 0.11 with increasing environmental gradient. Lower correlations among the sire classifications were obtained in comparison with HRNM1s in environments with low and high management, confirming the presence of genotype x environment interactions. Conclusion: We recommend a wider application of genetic evaluation in buffalo aimed at identifying optimal genotypes within specific environments.
Resumen Antecedentes: La cría de búfalos ha aumentado significativamente en Brasil en los últimos años. Sin embargo, se han realizado escasas evaluaciones genéticas. Objetivo: Evaluar las interacciones genotipo x ambiente en búfalos de agua Mediterráneos criados en Brasil, para peso a los 205 días de edad, utilizando modelos de reacción mediante regresión aleatoria. Métodos: Los datos de búfalos nacidos entre 1990 y 2014 se obtuvieron de cinco granjas situadas en el Norte (1), Nordeste (1), Sur (2) y del Sureste (1) de Brasil. Todas estas haciendas participan en el Programa Brasileño de Mejoramiento de Búfalos. Nuestra base de datos inicial consistió de 5.280 observaciones a los 205 días de edad (P205). Evaluamos el ajuste utilizando dos modelos de norma de reacción jerárquica: de dos pasos (HRNM2s) y un paso (HRNM1s). El ajuste del modelo se estimó usando el Criterio de información de la desviación, desviación basado en los factores de bayes y desviación basado en la ordenación predictiva condicional. Los descriptores ambientales fueron creados para agrupar individuos en ambientes de producción comunes basados en año, estación, rebaño y sexo. Resultados: El mejor ajuste se obtuvo para el modelo de norma de reacción jerárquica con un paso (HRNM1s). Las estimaciones de heredabilidad directa para este modelo variaron de 0,17 a 0,67 y la heredabilidad materna de 0,02 a 0,11 con gradiente ambiental creciente. Las correlaciones más bajas entre las clasificaciones de los reproductores se obtuvieron en comparación con las HRNM1s, en ambientes con bajo y alto manejo, confirmando la presencia de interacciones genotipo x ambiente. Conclusiones: Recomendamos la aplicación amplia de la evaluación genética en búfalos para identificar genotipos óptimos en ambientes específicos.
Resumo Antecedentes: A criação de búfalos aumentou significativamente no Brasil nos últimos anos. No entanto, eles raramente foram objeto de avaliações genéticas. Objetivo: Avaliar as interações genótipo x ambiente em búfalo Mediterrâneo criados no Brasil, para peso aos 205 dias de idade, utilizando modelos de reação por meio de regressão aleatória. Métodos: Os dados para búfalos de água do Mediterrâneo nascidos entre 1990 e 2014 foram coletados de cinco fazendas localizadas nas regiões Norte (1), Nordeste (1), Sul (2) e Sudeste (1) do Brasil. Todas essas fazendas participam do Programa Brasileiro de Melhoramento dos Búfalos. Nosso banco de dados inicial consistiu de 5.280 observações aos 205 dias de idade (P205). Nós avaliamos o ajuste usando dois modelos de norma de reação hierárquica: um de dois passos (HRNM2s) e um passo (HRNM1s). O ajuste do modelo foi estimado usando o Critério de informações do desvio, desvio baseado nos fatores de bayes e desvio baseado na ordenação preditiva condicional. Os descritores ambientais foram criados para agrupar indivíduos em ambientes de produção comuns baseados em ano, estação, rebanho e sexo. Resultados: O melhor ajuste foi obtido para o modelo de norma de reação hierárquica com um passo (HRNM1s). As estimativas de herdabilidade direta para este modelo variaram de 0,17 a 0,67 e a herdabilidade materna de 0,02 a 0,11 com gradiente ambiental crescente. As correlações mais baixas entre as classificações dos reprodutores foram obtidas em comparação com as HRNM1s, em ambientes com baixo e alto manejo, confirmando a presença de interações genótipo x ambiente. Conclusões: Recomendamos a aplicação mais ampla da avaliação genética em búfalos visando identificar genótipos ótimos em ambientes específicos.
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Screening the reference genes that were stably expressed under different light intensities for Viola yedoensis could provide reference for the related molecular research. In this study, 11 candidate reference genes were detected by RT-qPCR for tissues of V. yedoensis treated with different light intensities. Ge Norm, Norm Finder, Best Keeper, and Ref Finder website were used to comprehensively evaluate the reference genes, and verify the stability of the reference gene based on CAT1. Finally, the ideal reference gene was determined. The results showed that CYP, Actin, and SAMDC had small Ct value ranges and stable expression. Ge Norm demonstrated that CYP, SAMDC, and Actin were suitable reference genes. Norm Finder showed that the expression of α-TUB was the most stable. Best Keeper recommended CYP, Actin, and SAMDC as reference genes. Ref Finder assessed that SAMDC, CYP, α-TUB, and Actin had better stability, while GAPDH had the worst stability. The expression trend of CAT1 gene was consistent when calibrated with SAMDC, CYP, and Actin, while it was quite different from that calibrated with GAPDH. In summary, SAMDC, CYP, and Actin can be used as ideal reference genes for the gene expression profiling of V. yedoensis under different light intensities.
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Perfilação da Expressão Gênica , Reação em Cadeia da Polimerase em Tempo Real , Padrões de Referência , ViolaRESUMO
Abstract Background: Heat stress derived from global warming is causing major economic losses in the livestock industry. Objective: To develop a novel methodological approach for determining the influence of climatic factors on the estimation of genetic parameters for growth traits in Retinta cattle breed by using reaction-norm models. Methods: Live weight records (n=7,753) from 3,162 Retinta calves born from 1,249 dams and 85 sires and raised in the Andalusian region (Spain) were analyzed. The effect of heat stress was measured using the temperature-humidity index, calculated with climatological data obtained from four weather stations. A bivariate-random-regression reaction-norm model was used to estimate the (co)variance components of weight until weaning in two different climatic seasons corresponding to warm and cold months. Results: The heritability pattern of individuals reared under diverse environments during the first 90 days of age was different. However, differences were not significant at the end of the growing period. Weaned calves reared during the cold season showed greater growth from 70 to 160 days in comparison with those reared during the warm season. Conclusions: Overall, this assessment did not show significant effects of the genotype-environment interaction. However, highly significant evidence of genotype-climatic condition interaction was found during the calf´s first three months of age.
Resumen Antecedentes: El estrés térmico derivado del calentamiento global está causando pérdidas económicas en la industria ganadera. Objetivo: Desarrollar un enfoque metodológico para estimar la influencia de algunos factores climáticos sobre la estimación de parámetros genéticos en las variables de crecimiento de la raza bovina Retinta. Métodos: Se analizaron registros de peso vivo (n=7.753) de 3.162 terneros hijos de 1.249 vacas y 85 toros, criados en la región de Andalucía (España). El efecto del estrés térmico se midió mediante un índice de temperatura-humedad obtenido a partir de los datos de cuatro estaciones meteorológicas. Se usó un modelo bivariado de regresión aleatoria de "norma-reacción" para estimar la (co)varianza del peso hasta el destete en dos diferentes épocas climáticas correspondientes a los meses cálidos y fríos. Resultados: Se encontraron diferencias en el patrón de heredabilidad de individuos criados en diferentes ambientes durante los primeros 90 días de vida. Sin embargo, tales diferencias no fueron significativas al final del periodo de crecimiento. Los terneros destetados en la época fría mostraron un mayor crecimiento entre 70 y 160 días en comparación con los criados en la temporada cálida. Conclusiones: Se encontró una evidencia altamente significativa de la interacción genotipo-condición climática durante los primeros tres meses de crecimiento del ternero.
Resumo Antecedentes: O estresse térmico devido ao aumento da temperatura média está produzindo grandes perdas econômicas na indústria pecuária. Objetivo: Realizar uma nova abordagem metodológica para estimar a importância dos fatores climáticos em parâmetros genéticos em variáveis de crescimento da população da raça Retinta. Métodos: Foram analisados registros de peso vivo (n=7.753) de 3.162 Retinta bezerros, nascidos de 1.249 vacas e 85 touros criados na região da Andaluzia (Espanha). O efeito do estresse térmico foi medido utilizando o índice de temperatura-umidade obtido com dados climatológicos de quatro estações meteorológicas. Um modelo de regressão aleatória bivariado de norma-reação foi usado para estimar os componentes da (co)variância do peso à desmama em duas estações climáticas com correspondência aos meses quentes e fríos. Resultados: Diferenças foram encontradas no padrão de herdabilidade de indivíduos criados em diferentes ambientes nos primeiros 90 dias de idade. No entanto, essas diferenças não foram significativas no final do período de crescimento. Os bezerros desmamados globalmente na estação fria apresentaram um crescimento maior dos 70 aos 160 dias em comparação com os indivíduos criados na estação quente. Conclusões: Uma evidência altamente significativa da interação genótipo-ambiente foi encontrada durante os primeiros três meses de crescimento do bezerro.
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Background: The scope of this study is to evaluate information, behavior and attitude of antibiotics use of students in Golhisar Health Services Vocational School, Burdur Mehmet Akif Ersoy University.Methods: A questionnaire of 19 questions is applied to 701 students. In order to determine students’ attitude at antibiotics use; to evaluate attitude, subjective norm and intention, Likert scale consisting of multiple-choice questions were used.Results: Unattached sample test t showed that there is a significant difference in male and female students in subjective norm effecting antibiotic use and mean points of male students are higher than those of female students. It is found that antibiotic use due to attitude in 20 years and younger students than is significantly higher than 21 years old or older students. No significant difference determined among attitude, subjective norm and intention which effect use of antibiotics in terms of alcohol use, chronic disease and health insurance possession. As the programs that students are attending are evaluated, difference is observed only in attitude factor in this study. It is determined that attitude to antibiotic use of students of first and emergency aid program is lower than pharmacy services and child development programs.Conclusions: This study has shown that students are conscious about antibiotics use and their level of antibiotics use is low. Based on the results of the study, having individuals in the society educated about antibiotics use will substantially contribute to conscious use of antibiotics.
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Objective@#To evaluate annual skin equivalent dose and annual effective dose to consumers arising from three types of cloths samples containing naturally occurring radioactive material (NORM).@*Methods@#Firstly, measurement of radioactivity in three types of cloths were made with γ-ray spectrometer. And then, theoretical exposure scenarios were simulated with Monte Carlo software to calculate organ dose and effective dose by using ICRP reference voxel male phantom. Finally, annual skin equivalent dose and effective dose to consumers from these cloths samples were evaluated.@*Results@#In the wrapped and covered estimation models used in this study, sample mass ranged from 135 to 7 197 g, annual skin equivalent dose to consumers from these cloths samples from 155.41 to 9 028.61 μSv, and annual effective dose from 11.91 to 1 234.44 μSv.@*Conclusions@#NORM-added consumer products would cause some radiation hazard to human body.
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Objective To evaluate annual skin equivalent dose and annual effective dose to consumers arising from three types of cloths samples containing naturally occurring radioactive material (NORM).Methods Firstly,measurement of radioactivity in three types of cloths were made with γ-ray spectrometer.And then,theoretical exposure scenarios were simulated with Monte Carlo software to calculate organ dose and effective dose by using ICRP reference voxel male phantom.Finally,annual skin equivalent dose and effective dose to consumers from these cloths samples were evaluated.Results In the wrapped and covered estimation models used in this study,sample mass ranged from 135 to 7 197 g,annual skin equivalent dose to consumers from these cloths samples from 155.41 to 9 028.61 μ Sv,and annual effective dose from 11.91 to 1 234.44 μSv.Conclusions NORM-added consumer products would cause some radiation hazard to human body.
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O objetivo deste estudo foi analisar, de forma comparativa, os impactos da norma de responsabilidade social empresarial e a discriminação das mulheres negras, na indústria de transformação sergipana. Para tanto, foram utilizados os microdados da Relação Anual de Informações Sociais (RAIS) do Ministério do Trabalho e Emprego (MET) de 2007/2008 e 2013/2014. A partir da análise dos dados, nota-se, em relação à discriminação de gênero, uma tendência à predominância masculina no setor da indústria de transformação sergipana. Os dados indicam também que não apenas o sexo feminino é aquele com menor presença no setor industrial, mas é a mulher de cor preta que possui os menores percentuais de inclusão no setor industrial sergipano. Esse cenário comparativo de discriminação de gênero e raça pouco se alterou com a introdução da norma de responsabilidade social empresarial, a partir da década de 1990. Os resultados são discutidos de acordo com as teorias do sexismo e do racismo.
The aim of this study was to analyses comparatively the impact of social responsibility norm on black women discrimination in Sergipe's manufacturing industry, between 2007 and 2008 vs. 2013 and 2014. The data was collected from the Annual Report of Social Information (RAIS) of Ministry of Labor and Employment (MET). The results show a strong pattern of gender discrimination, indicating a male predominance in the Sergipe's manufacturing industry. The data also shows race discrimination, once the blacks were sub represented. Most important, was the intersection between gender and race, which demonstrates that black women had the lowest indexes of inclusion and salary in the industrial sector. This scenario of gender x race discrimination has changed little from 2007/08 to 2013/14, with the introduction of the social responsibility norm since the 1990s. The results were discussed in accordance to theories of sexism and racism.