The study on gastrointestinal transit and changes of myenteric nerve plexus in acute necrotic pancreatitis rats complicated with hyperlipemia / 中华消化杂志

Objective To explore association between the changes of myenteric nerve plexus and the delayed gastrointestinal transit in acute necrotic pancreatitis (ANP) rat complicated with hyperlipemia. Methods Atotal of 40 male Sprague-Dawley (SD) rats were randomly divided into 4 groups (normal control group, ANP group, hyperlipemia (HL) control group and HL with ANP group). HL rat model was established by fed with high-fat diet for 4 weeks, and ANP rat model was induced by retrograde injection of 3.5% sodium taurocholate into pancreatic duct. Gastrointestina1 transit distance was measured by ink gavage method. The histological changes of cholinergic and nitriergic nerves in myenteric plexus were observed by Karnovsky-Root method and NADPH histochemistry method. Results Pathological injuries were more severe in HL with ANP group than in ANP group (12.8±0.63 vs. 10.8±1.93,P<0.01), gastrointestinal transit was obviously delayed (transmission rate was 27%±5% vs. 38%±6%,P<0.01), the density of cholinergic neurons decreased (4.80±1.23 vs. 5.80±0.79, P<0.05), and the density of nitriergic neurons significantly increased (8.70±0.75 vs. 6.80±1.48, P<0.01). There was a linear regression between changes of cholinergic and nitrievgic nerves in myenteric nerve plexus and gastrointestinal transit (R2=0.531, P<0.01). Conclusion There was significant gastrointestinal motility disorder in the ANP rat complicated with hyperlipemia, which may be closely related with the changes of myenteric nerve plexus.

Changes in nitriergic nerves and interstitial cell of Cajal in intestinal myenteric plexus of scalded rat during early post-burn stage / 解放军医学杂志

Objective To study the changes in nitriergic nerves and interstitial cell of Cajal (ICC) in intestinal myenteric plexus of scalded rats during early post-burn stage. Methods 20 rats were randomly divided into scald group (n=10) and control group (n=10). A rat model of 30% TBSA with II degree scald was reproduced in the scald group. The rate of enteric transit was measured, and the histological changes in nitriergic nerves in intestinal myenteric plexus of scalded rat were observed as well. The densities of neurons and ganglions with positive nitric oxide synthetase (NOS) as well as ICC in duodenal myenteric plexus were assessed for both groups. Results In the rats in scalded group, the rate of enteric transit was prolonged, and the number of nitriergic nerves and ICC in myenteric plexus of rat duodenum were significantly decreased (P