Research progress of natural non-alkaloids with analgesic activity / 中国中药杂志

Pain is a complex, unpleasant feeling and emotional experience associated with actual or potential tissue damage, and manifests itself in certain autonomous psychological and behavioral responses. The commonly used opioid and non-steroidal anti-inflammatory analgesics(NSAIDs) may cause adverse reactions to the kidney, liver, cardiovascular or gastrointestinal system and cause problems of drug abuse. Therefore, it is necessary to study new analgesic drugs with less side effects and significant analgesic effects. A variety of natural products derived from terrestrial plants, microorganisms, marine organisms and fungi have been an important source of clinical medicines and provide an inexhaustible resource for the development and innovation of modern medicines. Therefore, this paper mainly reviews the natural non-alkaloids with analgesic activity in order to provide reference for the research and development of analgesic drugs derived from natural products.

Chemical constituents of non-alkaloids from Huperzia serrata / 中草药

Objective: To investigate the non-alkaloids in Huperzia serrata. Methods: The constituents were mainly isolated and purified by means of silica gel, RP18, and Sephadex LH-20 column chromatographies. The structures were identified based on analyses of the spectral data of NMR and MS. Results: Totally 11 constituents were obtained from dichloromethane fraction of the plant and they were 3β-hydroxyserrat-14-en-21β-yl-p-dihydrocoumarate (1), seratenediol-3,21-diacetate (2), seratenediol-3-acetate (3), 21-epi-serratenediol-3-acetate (4), 3α,21β,24-trihydroxyserrat-14-en (5), 21-epi-serratenediol (6), serratenediol (7), 3α,21β-dihydroxy- serrat-14-en-16-one (8), 3β,21β,24-trihydroxyserrat-14-en (9), 3α,21β,24-trihydroxyserrat-14-en-16-one (10), and 1-dibenzofuranol (11). Conclusion: Compounds 1-10 are serratene-type triterpenoids and compound 11 is a dibenzofuranol. Compounds 1 is a new compound named as serratcoumarate and compounds 2 and 11 are isolated from the plant for the first time.

Anti-inflammatory and antinociceptive activities of non-alkaloids fractions from Aconitum flavum in vivo

Aconitum flavum Hand.-Mazz., Ranunculaceae, has been used for the treatment of rheumatism, traumatic injury in folk and clinical medicine, but the alkaloids has high toxicity. This study was designed to investigate the acute toxicity, anti-inflammatory and antinociceptive activities of non-alkaloids fractions from A. flavum in rodents. The anti-inflammatory activity was evaluated by inflammatory models of dimethylbenzene-induced ear vasodilatation and acetic acid-induced capillary permeability enhancement test in mice and carrageenan-induced paw edema in rats whereas the antinociceptive activity was evaluated using acetic acid-induced writhes, hot plate test and formalin test in mice. The result showed that the LD50 value of BtOH and EtOAc fractions could not be determined as no lethality was observed up to 40 g/kg (p.o.) in mice. BtOH fraction significantly decreased the dimethylbenzene-induced ear vasodilatation, carrageenan-induced paw edema and acetic acid-induced capillary permeability. EtOAc fraction only significantly attenuated paw edema and capillary permeability at the dose of 500 mg/kg. In antinociceptive test, BtOH and EtOAc fractions significantly reduced the writhing number evoked by acetic acid injection and the licking time in both phases of the formalin test. Meanwhile BtOH and EtOAc fractions had significant effect on hot plate test after 90 min. Our data indicate that the BtOH and EtOAc fractions of NAF are no toxicity. BtOH and EtOAc fractions not only inhibit inflammatory and peripheral inflammatory pain but also have central antinociceptive effect.