RESUMO
Extract of Nauclea latifolia (NL) root bark collected from the Nigerian flora was examined for anti-RSV activity. Preliminary data showed anti-RSV activities with IC50 =75.62 µg/ml when tested against the recombinant strain rgRSV expressing the green fluorescent protein. Corresponding assays for the cytotoxic effect of the extract against utilized cell lines gave TC50 = 333.82 µg/ml. Further screening of against the circulating RSV A2 strain established their promising anti-RSV utility. Time of additional studies for the elucidation of the possible mechanism of action gave 74.38, 69.42, and 71.90% reduction of RSV plaque forming units at the respective 0, 2, and 4 hours post-infection addition times.
RESUMO
Objective:To assess the effects of hot water leaf extract of Nauclea latifolia (N. latifolia) on antioxidant status, lipid peroxidation values and parasite levels in hepatic and brain tissue of experimental mice (BALB/c) infected with Plasmodium berghei (P. berghei) malaria. Methods:Forty nine mice were divided into seven groups (n=7) and used for the study. Group A (control) were given 0.2 mL/kg phosphate buffer saline;Group B mice were infected with P. berghei and treated with phosphate buffer saline. Groups C and D mice were also infected but treated with 200 and 300 mg/kg body weight of leaf extract respectively. Groups E and F mice were not infected, but received 200 and 300 mg/kg of leaf extract respectively. Group G mice were infected and treated with chloroquine (5 mg/kg). Liver and brain tissues of mice were prepared for both biochemical assay and microscopic examination. Results:Results showed that P. berghei malaria infection induced oxidative stress in both liver and brain tissues as evidenced by the significant (P Conclusions:The bioactive phytochemical(s) in N. latifolia should be structured and the mechanism(s) of its antimalarial tendency should be further investigated.
RESUMO
Objective: To assess the effects of hot water leaf extract of Nauclea latifolia (N. latifolia) on antioxidant status, lipid peroxidation values and parasite levels in hepatic and brain tissue of experimental mice (BALB/c) infected with Plasmodium berghei (P. berghei) malaria. Methods: Forty nine mice were divided into seven groups (n = 7) and used for the study. Group A (control) were given 0.2 mL/kg phosphate buffer saline; Group B mice were infected with P. berghei and treated with phosphate buffer saline. Groups C and D mice were also infected but treated with 200 and 300 mg/kg body weight of leaf extract respectively. Groups E and F mice were not infected, but received 200 and 300 mg/kg of leaf extract respectively. Group G mice were infected and treated with chloroquine (5 mg/ kg). Liver and brain tissues of mice were prepared for both biochemical assay and microscopic examination. Results: Results showed that P. berghei malaria infection induced oxidative stress in both liver and brain tissues as evidenced by the significant (P < 0.05) decrease in antioxidants: superoxide dismutase, reduced glutathione and catalase. These reductions perhaps caused compromise in membrane integrity as indicated by the significant increase in lipid peroxidation product malondialdhyde. Malaria parasites were also identified in these tissues. However, N. latifolia treatment eliminated the parasites in tissues and protected them from oxidative damage even better than chloroquine treatment did, whose anti-malarial potency also cleared tissue parasites. The measurement of protection by N. latifolia against damage was strengthened by the insignificant micro structural alterations. Conclusions: The bioactive phytochemical(s) in N. latifolia should be structured and the mechanism(s) of its antimalarial tendency should be further investigated.