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Objective:To establish T lineage leukemia Jurkat cell mice model of over expression of C-terminal Src kinase binding protein( Cbp ) and Cbp palmitoylation and to research the effect of Cbp and Cbp palmitoylation to proliferation of Jurkat cell.Methods:Virus transfected cell of neg-EGFP,Cbp-EGFP and Cbp-m-EGFP were used in mice model.24 female BALB/c-nu mice were randomly divided into blank control group,empty virus control group,over expression of CBP group and Cbp palmitoylation group, 6 mice in each group.The nude mice were weighed in 0,1,2,3,4,5 weeks.The amount of white blood cell in peripheral blood were counted in 0, 1, 2, 3, 4, 5 weeks.The proliferation of Jurkat cell in peripheral blood of mice were observed by laser confocal microscope.The pathological changes of liver were observed using HE staining.The proliferation of Jurkat cell in the bone marrow and peripheral blood of mice were detected with flow cytometry.Results:The weight of mice in over expression of Cbp group was less than that in blank control group,but higher than that in empty virus control group and Cbp palmitoylation group.The weight of mice in Cbp palmitoylation group was less than that in blank control group,empty virus control group and over expression of Cbp group.The amount of white blood cell in peripheral blood and proliferation of Jurkat cell in liver, bone marrow and peripheral blood of mice in over expression of Cbp group was higher than that in blank control group, but less than that in empty virus control group and Cbp palmitoylation group.The amount of white blood cell in peripheral blood and proliferation of Jurkat cell in liver, bone marrow and peripheral blood of mice in Cbp palmitoylation group was higher than that in blank control group,empty virus control group and over ex-pression of Cbp group.Conclusion:Over expression of Cbp and Cbp palmitoylation in T lineage leukemia Jurkat cell mice model was established.Over expression of Cbp has inhibitory effect on the proliferation of Jurkat cell.Cbp palmitoylation has promotable effect on the proliferation of Jurkat cell.
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Objective To evaluate whether the functional MRI could be used to reflect the change of angiogenesis after drug treat-ment,and obtain the related semi quantitative and quantitative parameters by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI).Methods The subcutaneous transplantation colorectal cancer were constructed in 20 nude mice,which were treated with Xiaoaifeimi.The transplanted tumor microvascular density,VEGF and PCNA were monitored.The therapeutic effect and MRI monitoring results were evaluated by combining DCE-MRI with the pharmacokinetic model.The semi quantitative and quantitative parameters were obtained for evaluating the effection of medicine.Half of nude mice were sacrificed to obtain the immunohistochem-ical staining.Correlation between pathological findings and parameters were analyzed.Results Compared to the control group,hu-man colon HT-29 cell proliferation rate was significantly decreased (44.87%)(P <0.05),and the apoptosis rate of HT-29 cells was increased 2.45 times (P <0.05)after Uyghur medicine treatment.The correlation between pathological examination and DCE-MRI parameters showed that Ktrans value and Kep were positively correlated with VEGF,MVD and PCNA (P <0.05).Conclusion A good relationship is showed between immunohistochemistry and DCE-MRI quantitative parameters in Uygur drug treating human colon cancer mice.
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@#mPEG-SC20k-HM-3 is a novel anti-angiogenesis peptide with integrin affinity. To investigate the anti-tumor activities of mPEG-SC20k-HM-3 and oxaliplatin(OXA)combination, a transplanted tumor model of human hepatocellular carcinoma SMMC-7721 in nude mice was established. Jin′s formula to evaluate the combination effect was used. Data suggested that the anti-tumor activities of combined groups were better than those of single drug(P< 0. 05). Inhibition rate of group 8(OXA 7. 5 mg/kg and mPEG-SC20k-HM-3 73. 4 mg/kg)was 84. 6%, which showed remarkable superiority to group 3(OXA 7. 5 mg/kg)and group 4(mPEG-SC20k-HM-3 73. 4 mg/kg). The Q of group 8 was 1. 164(> 1. 15). This combination had synergistic effect. Combination of mPEG-SC20k-HM-3 and oxaliplatin is a method of inhibiting hepatocellular carcinoma.
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Objective:To explore the treatment effect of A-NK cells on the subcutaneously transplanted tumor of tongue squamous cell carcinoma in nude mice.Methods:Tca8113-Tb cells were subcutaneously transplanted into nude mice,14 tumor bearing mice were randomly assigned to 2 groups(n =7).The mice in control group were injected with normal saline,those in the experimental group were injected with A-NK cells.All animals were killed 33 days after tumor cell transplantation,The tumor volume and weight of the mice were measured and compared.Results:A-NK cells significantly inhibited tumor growth in nude mice in terms of the volume (P ＜0.05).After 15 -33 d of treatment the tumor weight(g)in the treated and control mice were 0.96 ±0.38 and 3.74 ±1.22 re-spectively(P ＜0.05).Conclusion:A-NK cells can inhibit the growth of Tca8113-Tb cell induced tumor in nude mice.
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Objective To explore the treatment effect and mechanism of A-NK cells on the subcutaneous trans?planted tumor of oral tongue squamous cell carcinoma in nude mice through observing the external growth and hyperplasia of A-NK cells and to provide theoretical evidence for squamous cell carcinoma treatment. Methods A-NK cells and NA-NK cells were both derived from healthy human peripheral blood and cultured in vitro. Cell growth was observed under micro?scope. The squamous cell carcinoma model in nude mice was established through subcutaneous implanting of Tca8113 cells. Then they were randomly assigned into three groups who were injected with either saline solution, or A-NK cells or NA-NK cells paraneoplastically. All animals were sacrificed after 33 days when tumor were isolated then weight and change in tumor size were assessed. Finally curve of tumor growth was drawn. Results Under the microscope, the proliferation of A-NK cells peak in 15 days and NA-NK cells peak in 12 days. After 3 weeks, the number of A-NK cells increased by 39.33 times while the number of NA-NK cells increased by 16.33 times. The Volume of tongue squamous cell carcinoma in saline solu?tion group was larger than that in A-NK cells and NA-NK cells groups, and volume in the NA-NK cells group was larger than that in A-NK cells group. The volume of tongue neoplasms in different groups, time, and interaction effects are statisti?cally significant (P<0.01). The tongue neoplasms weight in the saline solution group was greater than that in the A-NK cells and NA-NK cells group, and the weight in NA-NK cells group was greater than that in A-NK cells group, and the difference are statistically significant (P<0.05). Conclusion A-NK cells and NA-NK cells can significantly inhibit the subcutane?ous transplanted tumors in nude mice and the anti tumor effect of A-NK group is stronger than NA-NK.
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Purpose:To evaluate the effect of continuous intraperitoneal washing chemotherapy in treating the abdominal cavity tumor of nude mice with human ovarian epithelial cancer.Methods:The abdominal cavity tumor of nude mice models was established with human ovarian serous epithelial cancer cell line SKOV 3. 20 experimental nude mice were divided into 3 groups: the common treatment group(7 mice),the continuous washing intraperitoneal chemotherapy group(7 mice) and the control group(6 mice).All the mice were killed after two courses of treatment and the weight and volume of the cavity tumor were examined. The cell cycle expresstion rate of C erbB2 and Fas of abdominal neoplasms tissues in washing liquid were detected with FCM. Results:In the intraperitoneal washing group,the nude mice's FBW/IBW was 0.886. The volume of the cavity tumor is obviously smaller than the routine group. Significant difference in tumor growth inhibition rate was found between the routine group and the washing group.In the washing liquid, G 1 and S phase ratios were markedly lower ( P 0.05); C erbB2 rates were 66%、59% and 42% in control、common and washing group respectively, which was significant different between all groups ( P
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Objective To study the effects of angiogenesis inhibitor SU5416 on the growth and metastasis to the liver of gastric cancer and to investigate its effect on the apoptosis of gastric cancer cells. Methods Metastatic model simulating human gastric cancer was established by orthotopic implantation of histologically intact human tumor tissue into gastric wall of nude mice. Mice were randomly divided into 4 groups: control group (saline solution), 5 FU group (fluorouracil 30 mg?kg -1 ?d -1 i.p.), SU5416 group (SU5416 15 mg?kg -1 ?d -1 i.p.), and combined treatment of both 5 FU and SU5416 group. Eight weeks after implantation, the tumor weight, inhibition rates, intratumoral microvessel density (MVD), apoptotic index (AI), and the presence of metastasis were evaluated respectively after the mice were sacrificed. Results Compared with the control group, the growth of the orthotopically implanted tumor was significantly inhibited due to the reduced weight and the inhibition rate of tumor was 44.5%, 79.3%, and 84.4% respectively in mice treated with 5 FU, SU5416 and both. The incidences of liver metastases were also significantly decreased in the 5 FU group, SU5416 group, and combined group compared with those in control group (36.4%, 25.0%, and 0% vs 90.0%). The MVD was decreased significantly in the treated mice ( 14.6 ? 5.8 vs 13.1?4.7, 3.9? 1.8 , and 2.1?1.5). The AI was increased significantly in the treated mice [(3.76?2.25)% vs (6.81? 4.92 )%, (9.82?3.76)% and (17.65?9.85)%]. The growth and liver metastasis of human gastric cancer implanted in nude mice were more significantly inhibited in the SU5416 group and combined group than in control group and 5 FU group ( P