Content determination of alkaloids in different parts of Papaver nudicaule from different populations / 中草药

Objective To determine the qualitities Papaver nudicaule from different populations, a total alkaloid and three main kinds of alkaloids (reframidine, nudicauline, and amurensinine) from different parts, in order to provide a scientific basis for rational utilization and quality evaluation of P. nudicaule. Methods The qualitative and quantitative analysis of P. nudicaule has been conducted based on the method of LCMS-IT-TOF, Bromocresol green acid dye colorimetry and UPLC-MS, respectively. Results From the quantitative analysis, 13 kinds of alkaloids have been analyzed, in which eight kinds of chemical structures have been identified, and the results of quantitative analysis showed that there were greater variabilities in total alkaloid and three main kinds of alkaloids. Comprehensively, the plants grown in WLBT, HGL, and DL were better than other populations, in which the plants grown in WLBT were the best. In addition, the contents of total alkaloid and three main kinds of alkaloids were highly existed in the entire plants and flowers. Conclusion Based on the evaluation of the quality characteristics of P. nudicaule, it is suggested that total alkaloid and nudicauline can be seen as main reference indexes. Additionally, the result suggests that WLBT, HGL, and DL can be chosen to select and breed excellent quality of P. nudicaule, develop the quality standard and make layout of source area. The best harvest time should be chosen in late July to early August, and harvest site should be chosen flowers and whole plants.

Studies on harvest period for Papaver nudicaule / 中草药

Object To study the content of nudicauline in Papaver nudicaule L.from different harvest period, so as to provide the basis for development of the plant.Methods The nudicauline content in P.nudicaule was determined by HPLC with Shim-Pack CLC-ODS (4.6 mm?250 mm), mobile phase was methanol-water-diethylamine (550∶450∶0.45), detection wavelength was at 261 nm.Results The contents of nudicauline in P.nudicaule were greatly different from different harvest period, the content of nudicauline is higher in both July and Augest.Conclusion The whole plant is best harvested during July and Augest.