RESUMO
Aim: The study is aimed at assessing the antioxidant potentials of Nymphaea lotusand Nymphaea pubescensseed oils. Study Design:It was designed to extract oil from the seeds and investigate their antioxidant properties to indicate their capabilities to improve oxidative stability. Place and Duration of Study:The research was conducted between August 2016 and January 2017 atDepartment of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano State-Nigeria. Methodology: 2, 2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging and reducing power assays were used to determine free radical scavenging activities and reducing power potential of Nymphaea lotusand Nymphaea pubescensseed oils. Results:From the DPPH radical scavenging assay, It shows that the IC50value of the ascorbic acid (reference standard) was found to be higher (13.23± 0.16?g/ml) compared to Nymphaea lotusseed oil (8.82 ± 0.82?g/ml). The IC50 value of Nymphaea pubescensseed oil was also compared with the standard and was found to be (41.76 ± 0.98?g/ml) higher than ascorbic acid (13.23± 0.16?g/ml). The lower the IC50 value the higher the antioxidant activity of the oil. The order of the effectiveness of reducing capabilities of the oils was found to be N. lotusseed oil > ascorbic acid> N. pubescensseedoil.Gas chromatographic coupled mass spectroscopy analysis of the N. lotusseed oil showed that Dihydrocarveol (0.87%), Eicosane (1.10%), Lupeol (14.75%), Tetracosane (2.14%) and Viridiflorol (13.72%) were the major antioxidants compounds identified whereas Palmitic acid (23.57), Eicosane (7.65%), Oleic acid (37.85%), Tetracosane (3.10%) and Squalene (1.58%) were the major antioxidants detected in N. pubescensseed oil. Compared with standard (ascorbic acid), the results show that N. lotusseed oil possessed highest antioxidant potential. The higher antioxidant potential displayed by Nymphaea lotusseed oil could be attributed to the presence of these compounds that could act synergistically.Conclusion:Therefore, Nymphaea lotus seed oil and Nymphaea pubescensseed oil contains natural antioxidants that can improveoxidative stability.
RESUMO
Diabetes prevalence is one of the life threatening diseases in India. In this work we address a specific suitable ligand for diabetes mellitus. A large focus has been on structure based drug designing. Guggultetrol isolated from Nymphaea pubescenswas taken as ligand for molecular docking. A theoretical docking study, the evaluation of guggultetrol as inhibitor of Glucokinase (PDB ID: 1V4S) a validated drug target enzyme of the Type-II diabetes, was taken up. Guggultetrol was found to bind at active site of glucokinase with lowest binding energy and RMSD values to be -9.45Kcal/Mol and 2.0 Å respectively. Docking analysis of 1V4S with ligand enabled us to identify specific residues viz. Thr-168, Glu-290, Glu-51, Ser-411, Gly-410, Asn-254, Thr-206, Arg-155 and Asp-205 within the 1V4S binding pocket to play an important role in ligand binding affinity. The docking studies of the Guggultetrol with target protein showed that this is a suitable molecule which docks well with target related to diabetes mellitus. This compound has shown promising biological activity in preliminary studies by targeting multiple signaling pathways. Thus on the basis of our in silico studies we hypothesize that this compound into guggultetrol can be inhibitory effect on against diabetes. We concluded that the natural products with interesting biological properties and structural diversity have often served as valuable lead drug candidates for the treatment of human diseases.
RESUMO
The present study was designed to investigate the possible antidiabetic, hypolipidaemic and antioxidant effects of ethanol extract of Nymphaea pubescens tuber. Diabetes was induced in Albino rats by administration of alloxan monohydrate (150 mg/kg, body weight i.p). The ethanol extract of Nymphaea pubescens tuber at a dose of 200mg/kg and 500mg/kg body weight were administered at single dose per day to diabetes induced rats for a period of 14 days. The effect of ethanol extract of Nymphaea pubescens tuber extract on blood glucose, plasma insulin, urea creatinine, glycosylated haemoglobin, serum lipid profile (total cholesterol (TC), triglyceride (TG), low density lipoprotein- cholesterol (LDL-C), very low density lipoprotein- cholesterol (VLDL-C) , high density lipoprotein- cholesterol (HDLC) and phospholipids (PL)), serum protein, albumin, globulin, serum enzymes (Serum glutamate pyruvate transaminases (SGPT), serum glutamate oxaloacetate transaminases (SGOT) and alkaline phosphate (ALP)), lipoprotein peroxidation (LPO), blood reduced glutathione (GSH), oxidative glutathione (GSSG), GSH/GSSG ratio, erythrocytes glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione S-transferase (GST) were measured in the diabetic rats. The ethanol extracts of Nymphaea pubescens tuber elicited significant (p<0.05) reductions of blood glucose, lipid parameters except HDL-C, serum enzymes and significantly increased HDL-C and antioxidant. The extract also caused significant increase in plasma insulin (p<0.05) in the diabetic rats. In conclusion, ethanol extract of Nymphaea pubescens tuber offers promising antidiabetic and hypolipidaemic effects that may be mainly attributed to its potent antioxidant potential. Further studies will be needed in future in order to determine which one or more of its active constituents have the main antidiabetic and hypolidaemic effects.