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Objective To investigate the expression and clinical significance of DJ-1 protein in the endometrial carcinoma and delineate its functional roles in endometrial carcinoma progression.Methods Expression of DJ-1 was examined with immunohistochemical staining of 69 paraffin-embedded endometrial carcinoma samples who underwent surgery at Hunan province Tumor Hospital.Kaplan-Meier analysis was performed to describe the survival curve.Univariate and multivariate analyses were performed with COX proportional hazard model.Results (1) The expression of DJ-1 protein in endometrial carcinoma was significantly higher than that in normal endometrial tissues (P ＜ 0.05).(2) There was positive correlation between the high expression of DJ-1 protein in endometrial carcinoma samples and the pathological grade,the International Federation of Gynecology and Obstetrics (FIGO) stage,depth of myometrial invasion,or lymph node metastasis (P ＜ 0.05).(3) Kaplan-Meier analysis indicated that the cumulative 5-year survival rate in the low-DJ-1 expression group was 80.8％,whereas it was only 51.2％ in the high-DJ-1 expression group (P ＜ O.05).COX analysis indicated that the expression of DJ-1 was not the endometrial carcinoma independent prognostic factors for overall survival time (P ＞ 0.05).Conclusions The high expression of DJ-1 protein in endometrial carcinoma samples demonstrated a significantly positive correlation with the pathological grade,FIGO stage,depth of myometrial invasion,and lymph node metastasis.However,DJ-1 is not enough to be independent prognostic factor for overall survival time.
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ObjectiveTo study the expression and potential significance of NET-1 in endometrial carcinoma.Methods106 specimens after operation were selected.All specimens were stained with H.E.and immunohistochemistry.ResultsThe expression of NET-1 immunoreactivity were different in normal endometrium(43.8％ ),endometrial hyperplasia ( 79.2％ ) and endometrial carcinoma ( 100％ ) ( x2 =11.88,P ＜ 0.01 ).The expression of NET-1 in endometrial carcinoma was significantly higher than that in normal endometrium and simple endometrial hyperplasia ( x2 =36.14,10.86,P ＜ 0.01 ).The expression of NET-1 in endometrial hyperplasia was significantly higher than that in normal endometrium ( x2 =5.29,P＜ 0.05 ).There was a correlation between the expression of NET-1 and degree of endometrial lesions ( r =0.355,P ＜0.01 ).The expression of NET-1 was relative with differentiation (x2 =11.04,P ＜0.01 ; r =0.558,P ＜0.01),stages(x2 =11.04,P ＜0.01; r =0.558,P ＜0.01),depth of myometrial invasion (x2 =15.90,P ＜0.01 ; r =0.479,P ＜0.01),with or without metastasis in lymph node ( Z =-3.74,P ＜0.01 ),menopause condition ( Z.=-2.60,P ＜0.01).ConclusionsNET-1 may play an important role in carcinogenesis and progression of endometrial carcinoma.It might be a marker of biological behavior and reference for endometrial carcinoma gene therapy.
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ObjectiveTo discuss the expression of tumor shift suppressor gene KAI1,matrix metal proteinase 9 (MMP-9) in endometriosis sickness in dystopia internal membrane and its significance.MethodsIn 53 patients and 30 healthy controls,the expression of KAI1,MMP-9 in the endometriosis was detected with immunohistochemistry SP method,and the relationship was analyzed.ResultsKAI1 had low expression in EMS patients,while MMP-9 was highly expressed.In the control group,KAI1 was highly expressed while MMP-9 lowly expressed,and the differences between two groups were remarkably significant (P ＜0.05).KAI1 and the MMP-9 expression showed negative correlation between two groups.Conclusions KAI1 and MMP-9 was negatively expressed in EMS,which may play a role in the EMS prognosis.KAI1 and MMP-9 may be served as the prognosis and monitor markers for EMS.
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Objective To explore the effects of curcumin on the expression of C-erbB-2, Survivin and Bax in prost cancer cell. Methods The effects of curcumin on the expression of C-erbB-2, Suevivin and Bax were observed by SP immnohistocherminstry and cell cultivate methods. Results The expression of Survivin and C-erbB-2 in contrast group were strong, but the expression of Bax was very weak. After the curcumin were incubated with culture medium for 6h, 12h, 24h, 48h, the expression of C-erbB-2 and Survivin were gradually decreased in DU145 cells, while the expression of Bax was increased and reached highest at the time point of 12 ～24h. Conclusion The curcumin can reduce the expression of C-erbB-2 and surviving, and increase expression of Bax. The curcumin may exert antitumor activity by interacting with DU145 cells.