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Background: Harbouring of potential pathogens in operationtheatres (OTs) and intensive care units (ICUs) of hospital is amajor cause of patient’s morbidity and mortality. Environmentalmonitoring by the microbiological testing of surfaces andequipments is useful to detect changing trends of types andcounts of microbial flora. High level of microbial contaminationindicates the needs for periodic surveillance aimed at earlydetection of bacterial contamination levels and prevention ofhospital acquired infections.Aim: The aims of the study were to count CFU (colony formingunit) rate of indoor air, to identify bacterial colonization ofsurface and equipments isolated from Operation theatres, ICUsand Labour room of a teaching hospital in district Kangra,Himachal Pradesh.Methods: This retrospective study, analyzing themicrobiological surveillance data from OTs over a period of 2years from January2017 to December2018 was conducted at atertiary care hospital. Air sampling of 8 OT’s, 4 ICU’s and 1 LRwere done by settle plate method. Swabs were taken fromdifferent sites, equipments and bacterial species were isolatedand identified from them as per standard guidelines.Result: A total of 105 air samples were collected for 2 yearfrom 8 OT’s, 4 ICU’s and 1 LR. The bacterial CFU/m3 /mincounts of air from all OTs ranged from Superspeciality OTSshowed less bacterial CFU rate of air (0-5 CFU/m3) followed byOpthalmology OT (5-8 CFU/m3) and highest in Gynae (30-46CFU/m3). CCU showed less bacterial CFU rate (10-15CFU/m3) followed by Surgery ICU (28-35 CFU/m3) and highestin PICU (38-42 CFU/m3), Labour room showed 42-51 CFU/m3.Bacterial species were isolated from 43.85 % out of total 157swab samples taken from all OTs and ICUs. The mostcommon isolate was Bacillus species 46% followed by CONS(22%). Pathogenic organisms isolated were 10% Gramnegative bacilli which included 3% Non-Fermenters, thecommon isolate was Klebsiella spp. amongst gram negatives.
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Background: Nitrous oxide irreversibly oxidizes the cobalt atom of vitamin B12, inactivating it which is a co-factor for methionine synthase. Methionine is an essential aminoacid that serves as a methyl donor (via its activated from s-adenosyl methionine) in hundreds of biological reactions. Aim: The aim of the study was to evaluate Methionine Synthase (MTR) gene polymorphism in persons who are exposed to trace quantities of anaesthetic gases in operation theatres. Materials and methods: 87 cases of physical status American society of anaesthesiologists (ASA) I and II exposed to anaesthetic gases in operation theaters at Gandhi Hospital and Osmania General Hospital, Hyderabad were selected for study. Also 150 controls who were not exposed to anesthetic gases were selected at random to compare with the data generated on the subjects exposed to these gases. Results: This study entitled study of methionine “synthase (MTR) gene polymorphisms in personnel exposed to trace quantities of anaesthetic gases in operation theatres” was conducted on 87 exposed and 150 non exposed subjects. The objective was to evaluate the effect of Nitrous Oxide on Methionine Synthase (MTR) gene polymorphisms. No statistically significant difference was observed in the distribution of genotypes between the two groups. Conclusion: The result of the study suggest that large studies would be required to provide statistical power and recommends that examination should be done on large populations to assure at better conclusions.