Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion / Características Histoquímicas de la Matriz Extracelular en el Ganglio Ótico de Rattus rattus norvegicus

The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 µm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide + PAS. The presence of neutral glycoproteins was demonstrated by PAS reactivity. PAS inhibition following Alcian Blue staining in pH 2.5 and 1.0 showed the presence of a small quantity of acid glycoprotein. The extracellular matrix analysis showed the presence of neutral and acid glycoconjugates. These findings suggests a mutual interaction and a complex role in ganglionic physiology.

El ganglio ótico es un componente craneal de la división parasimpática del sistema nervioso autónomo (SNA). Similar al otro ganglio parasimpático, el ganglio ótico presenta neuronas multipolares que están rodeadas totalmente por las células satélites y la sustancia intercelular, lo cual permite la utilización de este ganglio como un buen modelo experimental para estudiar las relaciones matriz extracelular/neurona. Examinamos, con microscopio de luz, el ganglio ótico 10 ratones. Con los métodos histológicos rutinarios fueron obtenidas 5 secciones y coloreadas con tricrómico de Gomori, PAS, Azul de Alcián pH 2.5 y pH1.0, acetilación + PAS, acetilación + desacetilación + PAS, hidrólisis de ácido + PAS, fenilhidrazina + PAS y tiosemicarbacida + PAS. La presencia de glicoproteínas neutras fue demostrada por la reactividad de PAS. La inhibición de PAS y la posterior tinción con Azul de Alcian en pH 2.5 y 1.0, demostró la presencia de una cantidad pequeña de glicoproteínas ácidas. El análisis extracelular de la matriz demostró la presencia de glicoconjugados neutros y ácidos. Estos resultados sugieren una interacción mutua y un papel complejo en la fisiología ganglionar.

DISTRIBUTION AND ORIGINS OF THE NITRERGIC PERIVASCULAR NERVES IN RAT CEREBRAL ARTERIES / 解剖学报

Objective To observe the distribution and origins of the nitrergic perivascular nerves in rat cerebral arteries with a histochemical method for reduced nictinamide adenine dinucleotid phosphate(NADPH) diphorase activity, after resecting the membraneous structure at the ethmoidal formen. Methods Health adult SD rats were randomly distributed to three groups:sham operation group(group A), unilateral (right) operation group(group B), and bilateral operation group (group C). Both group B and C were further divided into two groups: group of resection of membraneous structure and nasociliary nerve (group BⅠ,CⅠ), and group of only removing membraneous structure (group BⅡ, CⅡ). On the seventh day after operation, the pterygopalatine ganglia and otic ganglia of group A were taken, and striped the cerebral arteries of three groups. With the method of NADPH d we investigated the positive cells in pterygopalatine ganglia, otic ganglia, and the changes of positive perivascular nerve fibers. Results A great deal of NADPH d positive cells were found in both pterygopalatine ganglia and otic ganglia. After unilateral operation, the NADPH d positive nerves in the ipsilateral middle cerebral artery(MCA) completely disappeared, but it did not change on the contralateral MCA. The positive nerves on basal artery(BA) and bilateral posterior cerebral artery(PCA), internal carotid artery(ICA), and anterior cerebral artery (ACA), obviously decreased. After bilateral operation, the positive nerve fibers in all arteries except ACA disappeared. There were no significant difference between group BⅠ and BⅡ; CⅠ and CⅡ.Conclusion There were nitrergic nerve fibers in all major cerebral arteries, and the density of positive nerve fibers in the anterior circulation of the cerebral artery was higher than that of posterior one. Different cerebral arteries have different origins of nitrergic nerve. The nitrergic nerve in BA, PCA and ICA completely originated from ipsilateral pterygopalatine ganglia; the nitrergic nerves of MCA were completely originated from ipsilateral pterygopalatine ganglion; ACA have more sources of nitrergic nerves other than bilateral pterygopalatine ganglia. Nasociliary nerve from trigeminal ganglion did not involve in the origins of nitrergic perivascular nerves in rat cerebral arteries. [