RESUMO
Objective: To study the inhibitory effect of ozone water on bad breath pathogens in vitro. Methods: In vitro cultured bad breath pathogens Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn) were identified by Gram stain and a PCR test. Ozonated water by was prepared by ozone generator and the concentration of ozone in water was measured using iodine titration. Artificial saliva was used to observe its interference on ozone function. Results: Gram stain and PCR results were consisted with strain characteristics. The ozone concentration of ozonated water reached to the maximum of 0. 2 mg/L. Ozone water with the concentration was 0. 05, 0. 1 and 0. 2 mg/L inhibited the proliferation of Pg, Pi and Fn. But the inhibitory effect was weakened when the concentration decreased. The artificial saliva reduced the effect of ozone water. Conclusion: The Pg, Pi and Fn can be inhibited by ozone water. Artificial saliva may reduce the effects of ozone water on the bacteria.
RESUMO
Objective To explore the clinical effects and application value of VSD combined with continuous hypoperfusion of ozone water in treating deep bedsore.Methods 40 patients with deep bedsore admitted to our hospital from June 2014 to December 2016 were randomly divided into the observation group (n=20) and the control group (n=20).The control group was treated with VSD while the observation group was treated with VSD combined with continuous hypoperfusion of ozone water.Results Compared with the control group, the wound healing time in the observation group was shortened significantly (P<0.001).The positive rate of bacteriology inspection about the wounds was decreased significantly (P<0.05).The density of capillary blood under microscope was increased significantly (P<0.05).Conclusion The clinical effects of VSD combined with continuous hypoperfusion of ozone water in treating deep bedsore is better than VSD alone apparently.It is worth clinically popularizing.