Epidemiological Studies on Leaf Blights of Maize Caused by Exerohilum turcicum (Pass.) Leonard and Suggs and Bipolaris maydis (Nisik. and Miyake)

The Field experiments conducted at Maize Research Station,Vagarai, Tamil Nadu, India during kharif 2019, 2020 & 2021 to study the disease development in relation to weather parameters, viz., temperature, relative humidity, rainfall with the leaf blights of maize. Observations on the spore load and disease grade were taken from 33rd standard week to 44th standard weeks at weekly interval. Increased spore load of 13 to 48 Nos. / Microscopic observations for TLB and 3 to 5 Nos./microscopic observations with the temperature ranges between 22-35?C, Humidity 48-72% and 19-24° Dew will lead to the TLB & MLB disease incidence with the grade of 1to 4 was observed. Based on the observations, the first appearance of leaf blight diseases were observed between 35th and 45th days after sowing with the grade of 1 (34th meteorological standard week). The disease grade increased from 1 to 4 as the age of the crop increases. There was a sudden increase in disease intensity because of increased scattered showers and increased relative Humidity (44th meteorological standard week). Temperature has not much influenced the disease development, since it was almost uniform throughout the cropping season in these three years. From the dataset, we would like to highlight that 34th and 44th meteorological standard weeks are highly critical for leaf blight disease development. Hence, spraying with mancozeb or zineb @ 2-4 g/l or propiconazole 25% EC @ 1ml/l during 34th and 44th meteorological standard week is recommended to manage the disease during kharif seasons in Tamil Nadu.

Delineation of Purple Blotch Disease Hotspots Caused by Alternaria porri in Key Onion Cultivation Regions of Southern Karnataka, India

Onion (Allium cepa L.) stands as a crucial bulb crop and a primary commercial vegetable crop in India. Among the various maladies affecting bulb and seed development, purple blotch, caused by Alternaria porri, stands out as one of the most devastating. This ailment inflicts extensive damage on both bulb and seed crops. Through an extensive roving survey, we have unveiled the disease's prevalence, enabling the identification of disease hotspots in the major onion cultivation regions of southern Karnataka. A total of 28 hotspot regions were identified in the eight districts surveyed for purple blotch disease severity in onions. Notably, the highest mean of percent disease index (PDI) was recorded in Chikkamagaluru (51.12%), while Chamarajanagara showed the lowest mean PDI (23.35%). Among all surveyed villages, the most severe disease prevalence was observed in Koverahatti village within Hiriyur taluk of Chitradurga district, with a PDI of 64.47%. On the other hand, the lowest disease severity was noted in Uttamballi village (16.89%) in Kollegala taluk, Chamarajanagara district.

Survey and Surveillance on Major Rice Diseases Severity in Karnataka, India

Rice blast, sheath blight and Grain discoloration diseases of rice were becoming a serious threat to rice crop in Karnataka. The patterns of spatio-temporal distribution of disease incidence were examined in two seasons (Kharif 2012 and 2013) with main objective of statues of rice diseases in paddy growing areas of Karnataka and variability with climatic condition. Most of the fields in the paddy growing areas of Karnataka were diseased, but significant differences in disease incidence occurred among the some sites in paddy growing areas of Karnataka. The leaf blast severity showed that the highest mean per cent disease incidence 37.20 was observed in Mandya district.and sheath blight severity showed that the highest mean per cent disease incidence 41.76 was observed in Yadgir district. The spatio-temporal distribution of grain discoloration incidence in Kharif 2012 and 2013 varied with districts, the maximum disease was in Bellary (33.82% and 31.24%) followed by Koppal (31.78% and 27.16%), for sheath blight, maximum disease was in Yadgir (36.23% and 41.76%) followed by Raichur (27.16% and 30.59%) and maximum leaf blast disease was in Mandya (37.20% and 36.91%) followed by Ramanagar (36.67% and 36.54%) respectively. Many fungi have been isolated from discolored grain, Curvularia lunata (Wakker) Boed. were found as a dominant pathogen. Survey and surveillance were serve as a precursor for evolving management strategies against the disease effective for the zone in an integrated way for sustainable development of crop in the state.

Psychological response and perception to lockdown among keralites during the COVID-19 pandemic: a cross-sectional study

Background: Kerala, which has a population density of 860 people per square kilometre, has been able to significantly curb the spread of COVID-19. This was a result of people in the State, politics, and social settings. Previous outbreaks showed that psychological symptoms could be severe and last longer than physical illness. This study examines the sources of information as well as the peritraumatic distress, attitude towards lockdown. Methods: 2335 individuals under lockdown were given a 32-item questionnaire over the internet from March to August 2020. This covers demographic data, days spent in quarantine, attitude, and awareness, adherence to lockdown procedures, and PDI for measuring psychological distress. Overall score was calculated and associations found using the Chi-square test in SPSS 20. Results: The average quarantine period lasted 10.12 days. 77.9% of individuals reported high stress, with a mean PDI score of 47.73. The psychological distress was more severe for those who were female and were placed in quarantine. The PDI score has associations with gender, education, health care access, loneliness, and the propensity to not adhere to lockdown measures. 89% of the responders expressed concern for their safety. We discovered that being in quarantine had a significant association with loneliness, concern about getting medical care, and resource accessibility. Conclusions: The population had high levels of perceived stress during the pandemic. The detrimental effects of COVID-19 do not just fit into morbidity and mortality but have wider implications for mental health issues that require proper attention.

Progression of Powdery Mildew of Indian Mustard (Brassica juncea L.) in Relation to Weather Parameters

The work entitled “Progression of powdery mildew of Indian mustard (Brassica juncea L.) in relation to weather parameters” was carried out in Agriculture Research Farm, Banda University of Agriculture & Technology, Banda, Uttar Pradesh, India during Rabi 2020-21 season. As infection and development of a disease depends on the favourable environmental conditions hence, relationship between powdery mildew of Indian mustard and weather factors was evaluated in Ganga, Varuna, Giriraj and RH-0749 varieties of Indian mustard in which maximum temperature between 25-30°C, minimum temperature >10°C with minimum relative humidity (RH) <50% favoured the development of powdery mildew in all the four varieties Indian mustard varieties. Individual and combined effects of different weather parameters on development of powdery mildew was evaluated using correlation and regression analysis. Percent disease index (PDI) of powdery mildew was positively correlated with the maximum and minimum temperature whereas, it was negatively correlated with maximum RH, minimum RH and rainfall in Indian mustard varieties Ganga, Varuna, Giriraj and RH-0749. Coefficient of determination (R2) explained that 96, 96, 95 and 96 % powdery mildew development was influenced by the maximum temperature, minimum temperature, maximum relative humidity, minimum relative humidity and rainfall in Indian mustard varieties Ganga, Varuna, Giriraj and RH-0749, respectively. Multiple linear regression equation was developed to find out the expected PDI of the disease based on the predictor weather variables. Correlation analysis explained that warmer temperature and lesser humidity favoured the development of powdery mildew in Indian mustard. Multiple linear regression model developed in current investigation may be utilized for development of powdery mildew prediction model in Indian mustard.

Application of the artificial neural network to optimize the formulation of self-nanoemulsifying drug delivery system containing rosuvastatin

The objectives of this study were to optimize the formula of the self-nanoemulsifying drug delivery system (SNEDDS)containing rosuvastatin and to evaluate its physicochemical characteristics. The solubility and compatibility ofrosuvastatin in surfactants, cosurfactants, and oil excipients were evaluated. The D-optimal experimental design,created by JMP 15 software, was used for analyzing the effects of excipients on the physicochemical characteristicsof SNEDDS to optimize the rosuvastatin SNEDDS formula. The generated nanoemulsions from Ros SNEDDS werecharacterized for droplet size, polydispersity index, and entrapment efficiency. As a result, Cremophor RH40, Capryol90, and PEG 400 were selected to develop the pseudoternary phase diagram to identify the area capable of selfforming nanoemulsion. As the percentage of rosuvastatin calcium increased from 8% to 12%, the area for optimizingthe formula of Ros SNEDDS decreased. The Ros SNEDDS prepared according to predicted formulas possessed selfemulsification to form nanoemulsion with average droplet size less than 100 nm, polydispersity index less than 0.3,and rosuvastatin entrapment higher than 90%.

Formulation, Optimization And Characterization Of Nanostructured Lipid Carrier Of Nitrofurantoin

Nitrofurantoin is effective against many urinary tract pathogens. It acts as bacteriostatic and/or bactericidal by inhibiting DNA-RNA protein& cell wall synthesis. Nanostructured Lipid Carriers (NLCs) of NFT was prepared by Hot Homogenization Process. Glyceryl Monostearate and Miglyol 812 were heated at 80ºC temperature on hot plate. In the melted lipid, drug was added with continuous stirring at high speed homogenization. Formulation NLC12B has % Entrapment efficiency 89.1 ± 0.5, PDI 0.11 ± 0.01 and mean particle size 237 ± 7nm represents narrow particle size distribution. Spherical feature of NLCs with better uniformity without aggregation of Nitrofurantoin loaded NLC was confirmed by TEM. Moreover, efficient miscibility of drug in lipids was confirmed by the absence of intense and characteristic peak of NFT in XRPD. After 6 month storage at 2-8°C there was no significant changes in the PDI as well as mean particle size.

Formulation optimization of rutaecarpine lipid liquid crystalline nanoparticles by Box-Behnken design-response surface methodology / 中草药

Objective To optimize the formulation of rutaecarpine lipid liquid crystalline nanoparticles (Rut-LLCN) by Box-Behnken design-response surface methodology. Methods Rut-LLCN were prepared by precursor injection-high pressure homogenization method. A three factor and three-level Box-Behnken design was employed with the glyceryl monoolein quality, percentage of poloxamer in glyceryl monoolein and the rutaecarpine quality as independent variables, the entrapment efficiency, drug loading, mean particle size and polydispersity index as the dependent variables to sereen the optimal formaula. Results Optimized prescription was GMO 450 mg, F127-GMO 12%, and Rut 20 mg. All items of optimized prescription were similar to target values. According to the optimized prescription, the entrapment efficiency, drug loading, average particle size, and PDI of Rut-LLCN were (84.02 ± 7.99)%, (3.24 ± 0.30)%, (186.90 ± 13.50) nm, and 0.313 ± 0.020, respectively. Conclusion The prescription optimization model of Rut-LLCN was optimized by Box-Behnken designs-response surface methodology, and entrapment efficiency, drug loading, mean particle size, and PDI of Rut-LLCN are measured to investigate the model.

Technology research and quality assessment on prescription of imperatorin lipid microspheres by central composite design-response surface method / 中草药

Objective To optimize prescription and evaluate the quality of imperatorin lipid microspheres (ILM) by central composite design-response surface methodology (CCD-RSM), therefore to use the mean particle size, particle size distribution (polydispersity index, PDI) and Zeta potential as the primary indicator. Methods Mean particle size, particle size distribution and Zeta potential of lipid microspheres were measured to investigate the effect of the concentration of egg yolk lecithin, poloxamer and percentage of soybean oil in oil phase on the properties of ILM preparation. Mathematic relation between indicator and factor was constructed by binominal fitting. Then the response surface method according to the best mathematical model of evaluation indicators was used to make predictive analysis of the best prescription. Optimized prescription was used to prepare ILM and evaluate its quality. Results Optimized prescription was egg yolk lecithin 1.50 g, F68 0.35 g, and MCT 10 g. All items of optimized prescription were similar to target values. According to the optimized prescription, mean particle size of ILM was (165.00 ± 0.22) nm, particle size distribution was 0.046 ± 0.070, Zeta potential was (−30.30 ± 0.13) mV, encapsulation efficiency was about 90.09%, drug-loading rate was 1.0 mg/mL. Conclusion Due to the better predictability of constructed mathematical model, CCD-RSM can be applied to optimize prescription of lipid microspheres and the optimized ILM meet pharmacy requirements.

O Palco Regulatório das Patentes Farmacêuticas: Licença Compulsória como Ferramenta Regulatória apoiada em PD&I / The Regulatory Stage of pharmaceutical patents: Compulsory Licensing as a RD&I-based Regulatory Tool / El escenario regulatorio de las patentes farmacéuticas: la licencia obligatoria como herramienta regulatoria apoyada en I + D + i

O direito à saúde garantido por intermédio do acesso a medicamentos estratégicos passou por um teste decisivo no início da década de 2000, quando os Estados Unidos e a União Europeia se posicionaram em frontal colisão com países em desenvolvimento, em especial África do Sul e Brasil, sobre a extensão do instituto jurídico da licença compulsória prevista no Acordo TRIPS. O presente artigo insere a licença compulsória e o investimento público em PD&I farmacêutico como instrumentos de estratégias regulatórias que contracenam no palco sociológico do direito ao configurarem mais do que reações pontuais a deficiências de mercado de fármacos ou de crises de saúde pública. Tais medidas de licença compulsória e investimento em PD&I farmacêutico representam atores de um palco regulatório que se descortina como um todo coerente voltado à administração do direito à saúde mediante uso da ameaça de licença compulsória para bloqueio de aumento arbitrário de preços de medicamentos e uso do investimento em PD&I para reforço da medida regulatória de quebra de patentes de medicamentos. A proposta defendida neste artigo é a de que o quadro regulatório da defesa da saúde pública foi melhor esboçado quando a licença compulsória deixou de representar a solução definitiva para se apresentar como a ultima ratio de modelagem regulatória após o reforço da condição de produção local de medicamentos via investimentos públicos em PD&I.

Access to strategic medicines as a guarantee of the right to health was submitted to a decisive test in the first half of 2000s, when the US and Europe positioned themselves in frontal collision with several developing nations, particularly South Africa and Brazil, on the topic of extension of the legal construct of compulsory license inaugurated by the TRIPS Agreement. This paper puts side-by-side the legal construct of compulsory license and RD&I investment in the pharmaceutical sector as regulatory tools prone to interact in the law sociological stage, as they present themselves as acts of an encompassing regulatory scheme. The regulatory stage makes use of off-stage characteristics of the socioeconomic environment to set up those tools in different ways. Compulsory license has been used as a threat that blocks arbitrary price increase of drugs, while RD&I investment has been applied to counteract the big pharma expectations of state inability to produce drugs following specific threats to break patented pharmaceutical inventions. This paper addresses the role of RD&I investments towards drug development to modulate the efficacy of compulsory licenses.

El derecho a la salud garantizado a través del acceso a la medicación estratégica se sometió a una prueba de fuego en la década de 2000, cuando Estados Unidos y Europa se han posicionado en colisión frontal con ciertos países en desarrollo, particularmente África del Sur y Brasil, alrededor de la extensión del instituto jurídico de las licencias obligatorias previsto en el Acuerdo TRIPS. Este artículo inserta la licencia obligatoria y la inversión en I + D + i como instrumentos farmacéuticos estratégicos de regulación que contraseñan en el escenario sociológico jurídico configurando más do que una respuesta puntual a las deficiencias del mercado de las drogas o las crisis de salud pública. Tales medidas de licencias obligatorias y de inversión en I + D + i farmacéutica representan actores de una etapa de regulación que se desarrollan como un todo coherente centrado en la administración del derecho a la salud a través del uso de amenazas de licencias obligatorias para bloquear el aumento arbitrario de precios de los medicamentos y el uso de la inversión en I + D + i para fortalecer la regulación de las patentes de medicamentos. La propuesta presentada en este artículo es que el marco regulador de la protección de la salud pública se ha definido mejor cuando la licencia obligatoria ya no representa la solución definitiva sino la última ratio en la estrategia de regulación basada en inversiones en I + D + i

mRNA and Protein levels of rat pancreas specific protein disulphide isomerase are downregulated during Hyperglycemia.

Diabetes (Type I and Type II) which affects nearly every organ in the body is a multi-factorial non-communicable disorder. Hyperglycemia is the most characteristic feature of this disease. Loss of beta cells is common in both types of diabetes whose detailed cellular and molecular mechanisms are yet to be elucidated. As this disease is complex, identification of specific biomarkers for its early detection, management and devising new therapies is challenging. Based on the fact that functionally defective proteins provide the biochemical basis for many diseases, in this study, we tried to identify differentially expressed proteins during hyperglycemia. For that, hyperglycemia was induced in overnight fasted rats by intra-peritoneal injection of streptozotocin (STZ). The pancreas was isolated from control and treated rats for subsequent analyses. The 2D-gel electrophoresis followed by MALDI-TOF-MS-MS analyses revealed several up- and down- regulated proteins in hyperglycemic rat pancreas including the downregulation of a pancreas specific isoform of protein disulphide isomerase a2 (Pdia2).This observation was validated by western blot. Quantitative PCR experiments showed that the level of Pdia2 mRNA is also proportionally reduced in hyperglycemic pancreas.

Functional and Evolutionary Genomics of Protein Disulphide Isomerase (PDI) Geneb Family in Wheat and Its Wild relatives: A Review.

Mature wheat grain contain 8-20% proteins. The storage proteins of wheat determine the characteristics and unique properties of its flour in the process of pasta and bread-making and eventually the quality of the finished products obtained. The storage proteins play an integral role in determining the visco-elastic properties of wheat dough, a feature of high quality wheat. The genes, encoding storage proteins as well as factors that may affect their deposition, such as molecular chaperones and foldase enzymes, are of particular interest to wheat industry. Genomic, cDNA and promoter sequences of the three homoeologous gene encoding the “typical” PDI has been already cloned and characterized. Recently, eight new non-homoeologous wheat genes were cloned and characterized in bread wheat. Study characterizing the variability in a 700 bp region comprising 600 bp of 5’ upstream putative promoter region and 100 bp of the first exon of the typical PDI gene in 15 accessions of Triticum urartu (AA), Aegilops speltoides (BB) and Aegilops taushcii (DD), from diverse origin and Triticum aestivum cv Chinese Spring (AABBDD) has also been completed recently. This review explains how functional and evolutionary genomics tools aid in the study of a gene family and its promoter.

A Case of Transfusion Transmitted Hepatitis A Virus Infection Identified Through a Lookback Study / 대한수혈학회지

Hepatitis A virus (HAV) is mostly transmitted by the oral-fecal route. However, rare cases of transmission via blood transfusion still exist. A retrospective study was designed to trace a case of transfusion transmitted HAV (TTHAV) infection through the Post Donation Infection (PDI) system in Korea in 2006. We report here on a case of TTHAV that was identified through a lookback study. A 35-year old woman received 6 units of packed red cell transfusion when undergoing Caesarian section in a hospital. Ten days later, she developed a high fever and sore throat. Anorexia and abdominal pain developed in 45 days after transfusion. She was diagnosed with acute tonsillitis at the hospital, and she was not aware of her HAV infection at that time. Then, a donor informed the blood center of his HAV infection 10 days after donation to request his blood be discarded. The recipients of the blood were traced and epidemiological studies lead to the patient, which prompted her serum to be collected and tested. Finally, TTHAV was identified for this patient with positive anti-HAV IgG/IgM results. This is the first case of TTHAV to be identified through the HAV lookback study in Korea.

Research progress in the relationship of autocrine motility factor,autocrine motility factor receptor and the nervous system / 中国药理学通报

Autocrine motility factor (AMF) plays an important role in the stimulation of the migration and motility of cells, especially the generation, migration and angiogenesis of tumor. Recently, it has been found that AMF has three isoforms, ATX-t, ATX-m and PD-I alpha. The PD-I alpha isoform is specifically expressed in the brain, which plays extensive functions in nervous system, such as regulating neural development and differentiation, promoting neurotrauma repair, inducing neuropathic pain, even contributing neurodegeneration under some circumstances. This indicates the close relationship of AMF/AMFR and the pathophysiology of the nervous system. This paper mainly reviews the function of AMF and AMFR and its possible mechanism in the nervous system.

The Negative Effect of Cigarette Smoking on Periodontal Therapy / 中国医师杂志

Objective To investigate the negative effect of cigaratte smoking on periodontal theraphy. Mothods 84 patients with periodontopathy were selected and divided into two groups. The smoking group (group A) included 33 patients and the non-smoking group (group B) included 51 patients. All the patients received a systematic treatment. The effects of the treatment between different groups were compared through the debris index(DI),calculus index(CI),periodotal disease index(PDI) which were respectively recorded before and after treatment.Results After the treatment the DI,CI,PDI of two groups were all decreased; wheras compared with group A, every index of group B was significantly lower. Conclusions Somking seriously influences the effect of periodontal therapy. Therefore, to give up smoking is very important for preventing and treating periodontopathy.

Protective Effect of Shengmai Injection on Adriamycin-induced Cytotoxicity of Diaphragm / 中药新药与临床药理

To study the protective effect of Shengmai Injection on adriamycin-induced cytotoxity of diaphragm in rabbits. The rabbits were randomly allocated to three groups. The control group were treated with NS 2 ml?kg -1 for five days, adriamycin+NS group with NS 2ml?kg -1 for five days and then administered adriamycin 10 mg?kg -l and adriamycin + Shengmai Injection group with Shengmi Injection 2 ml?kg -l for five days and then administered adriamycin 10 mg?kg -1 . Twenty-four hours later, animals in the three groups were anaesthetized. Then the transdiaphragmatic pressure (Pdi) and diaphragm evoked potential(DEP) were measured, SOD activity and MDA content were tested and the ultrastructure of diaphragm cells was observed under electron microscope. Adriamycin could lower the Pdi and amplitude of DEP (P