Methods of plant growth-promoting fungi application to enhance the growth and yield of wheat var. Ibaa99

Aims@#The main aim of the study was to evaluate some methods of application of Aspergillus niger AD1 and Trichoderma hamatum T-113 for enhancing the growth and yield of wheat var. Ibaa99 in pots and field conditions.@*Methodology and results@#Plant growth-promoting fungi (PGPF) loaded with peat moss were used at a rate of 100, 150 and 200 mL pot-1 or m-2 in filed soil; seed treatment (coating) with fungi suspension 19 × 107, soil treatment and combination of all the three methods was employed in the study. Wheat seeds were sown in pots and field plots during 2018-2019, and data regarding various growth and yield attributes were recorded. In both pot and field trials, the results revealed that the best treatments for the desired plant growth and yield attributes were peat moss 150 mL alone or in combination with soil and seed treatments. The soil physicochemical parameters were also improved after inoculation with selected fungal isolates in different application methods compared with un-inoculated control treatment in both pot and field conditions.@*Conclusion, significance and impact of study@#The PGPF play a vital role represented phytoremediation, phytostimulation and bio-fertilization. The isolates of PGPF, which were applied with peat moss at 150 mL to the pot and in the field alone or combined with seed treatment and soil application, were significantly the best effective method for improving wheat attributes.

Effect of plant growth promoting fungal inoculant on the growth of arachis hypogea (L.) and it’s role on the induction of systemic resistance against rhizoctonia solani.

Beneficial plant microbe interactions in the rhizosphere are primary determinants of plant health and soil fertility. Plant growth promoting fungi have great effect towards the growth of plant crops. Soil borne pathogenic fungi cause heavy crop losses all over the world. As the use of chemicals for disease control and fertilization causes environmental problems, there is a need for alternative control measures. The most important and economically cultivated plant pea-nut was selected to test the growth promotion by antagonistic microorganisms with or with our pathogen Rhizoctonia solani . This necessitates a study on Plant Growth Promoting Fungi (PGPF) as adequate work has gone on rhizobacteria. The present investigation was conducted to study the effect of PGPF on the growth of Arachis hypogea (L.) and its role of induction of systemic resistance against Rhizoctonia solani. Forty five rhizosphere fungal isolates were obtained from 12 different cultivated field crops and were screened for their potential to promote growth in Arachis hypogea (L.). The isolate (Cc2) obtained from Cucumis sativus (L.). Duch.ex. poir was identified as the potential plant growth promoting fungus. The effect of soil inoculation with the selected isolate Cc2 on the growth of healthy plants of Arachis hypogea (L.) and those challenged with Rhizoctonia solani was studied by pot culture experiment. The overall vegetative growth of plant (Root and Shoot development, Dry matter accumulation) and reproductive growth (Pod and Seed development) were studied. The fungal inoculants improved effectively the growth both in plants challenged and unchallenged with Rhizoctonia solani. The soil inoculation of Cc2 has improved the chlorophyll, carotenoid, anthocyanins ,total soluble sugar and protein content compare to the untreated plants (T0) and plants infected by Rhizoctonia solani (T1). The phenol and proline contents were found to be higher in plants challenged with Rhizoctonia solani. Growth hormone production ability of the selected isolate was determined. Results revealed that the selected isolate could produce Indole Acetic Acid and Gibberellic Acid. The in vitro study by dual culture method revealed that there was a negative interaction (Antibiosis) between the plant growth promoting fungal inoculant (Cc2) and the pathogen Rhizoctonia solani. It could be concluded that the selected isolate Cc2 proved to be a potential fungus in promoting plant growth and yield in Arachis hypogea (L.) and in inducing systemic resistance in Arachis hypogea (L.) against Rhizoctonia solani. Finally Cc2 was identified as Rhizopus sp., in generic level.